In this short review, a reliability of electrothermal atomic absorption spectrometry (ETAAS) for the quantification of trace selenium in blood serum is described and discussed. The main attention is given to the measurement conditions, especially those of pyrolysis temperature, in order to avoid possible pre-atomization loss of selenium; and spectral interferences that can be expected to be encountered when analyzing blood serum. Optimization of both the measurement conditions and the operating instrumental conditions facilitates the achievement of reliable results.