Direct determination of methamphetamine enantiomers in urine by liquid chromatography with a strong cation-exchange precolumn and phenyl-β-cyclodextrin-bonded semi-microcolumn

Simultaneous determination of methamphetamine and its metabolite, amphetamine, in urine using a high performance liquid chromatography column-switching method

“…Most of the methods involve sample pretreatment by extraction as well as through a concentration process. A few studies reported a column-switching HPLC-UV method to screen out many drugs including AM [12], and a columnswitching HPLC-UV method for the determination of MA enantiomers in urine [10]. The column-switching HPLC-UV method used in the present study is the first approach that simultaneously determines both MA and AM in urine.…”

“…Automated forensic analysis methods using HPLC to determine drugs in a biological matrix have also been recently reported [10][11][12][13]. These methods involve the capacity to extract, separate, and detect drugs in urine or serum via an on-line connection with or without mixing with a buffered solution.…”

Analysis of amphetamine-type stimulants and their metabolites in plasma, urine and bile by liquid chromatography with a strong cation-exchange column-tandem mass spectrometry

“…In addition, compounds with aliphatic amine moieties can be effectively separated and identified by means of an LC with a strong cation-exchange column-tandem MS (LC/SCX-MS/MS). An SCX column has the advantage of good retention of compounds with aliphatic amine moieties such as ATSs [11,17] and has a low affinity for compounds without aliphatic amine moieties. This enables the elimination of unnecessary matrices and compounds without aliphatic amine moieties, which are eluted at early retention times by changing the valve from an MS line to a waste line.…”

On-Line Coupling of Solid Phase Extraction Sample Processing with High-Performance Liquid Chromatography

“…The elution conditions and switching time are optimized to transfer a heart-cut fraction containing all analytes. [22][23][24][25] In this system it is possible to use a bigger size of precolumn. When the dimensions of both columns are comparable the method is called "column-coupling" 26 or "twodimensional chromatography".…”

“…Immunosorbents and molecular imprinted materials, which are based on molecular recognition, also allow achieving isolation and preconcentration in a single step. On-line SPE sample processing is very well suited for analysis of biological fluids such as urine [7][8][9][10]15,[20][21][22][23][25][26][27]30,31,37,39,[44][45][46]59,[94][95][96][97][98][99]102 and plasma. 7,8,15,16,18,20,22,33,36,37,56,57,69,[70][71][72][73]94,96,100 The determination of various pharmaceutics and drugs of this kind of matrices is required for pharmacokinetic and bioavailability studies 7,8,15,16, 30,38, 45,56, …”