Direct determination of glucose, lactate and triglycerides in blood serum by a tunable quantum cascade laser-based mid-IR sensor

Brandstetter, Markus; Volgger, Lukas; Genner, Andreas; Jungbauer, Christof; Lendl, Bernhard

doi:10.1007/s00340-012-5080-z

Cited by 78 publications

(67 citation statements)

References 27 publications

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“…The kidney tissue example presented in this paper demonstrates the importance of higher spatial resolutions in order to extract biochemical information from glomerular structures (Figure 3 and Figure 5). In the future, new advances in Quantum Cascade Lasers as very bright IR light sources [54][55][56][57] , 3D spectral imaging 58 , and breakthroughs in the field of nanoscale IR technologies 52,53,59,60 hold exciting new avenues of research that may have huge implications in the future of tissue imaging.…”

Section: Representative Resultsmentioning

confidence: 99%

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Sreedhar

Varma

Nguyen

et al. 2015

JoVE

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High-definition Fourier Transform Infrared (FT-IR) spectroscopic imaging is an emerging approach to obtain detailed images that have associated biochemical information. FT-IR imaging of tissue is based on the principle that different regions of the mid-infrared are absorbed by different chemical bonds (e.g., C=O, C-H, N-H) within cells or tissue that can then be related to the presence and composition of biomolecules (e.g., lipids, DNA, glycogen, protein, collagen). In an FT-IR image, every pixel within the image comprises an entire Infrared (IR) spectrum that can give information on the biochemical status of the cells that can then be exploited for cell-type or disease-type classification. In this paper, we show: how to obtain IR images from human tissues using an FT-IR system, how to modify existing instrumentation to allow for high-definition imaging capabilities, and how to visualize FT-IR images. We then present some applications of FT-IR for pathology using the liver and kidney as examples. FT-IR imaging holds exciting applications in providing a novel route to obtain biochemical information from cells and tissue in an entirely label-free non-perturbing route towards giving new insight into biomolecular changes as part of disease processes. Additionally, this biochemical information can potentially allow for objective and automated analysis of certain aspects of disease diagnosis.

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Section: Representative Resultsmentioning

confidence: 99%

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Sreedhar

Varma

Nguyen

et al. 2015

JoVE

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“…A tunable laser was employed as the light source to improve the measurement resolution of the designed sensor because of the laser's high peak power and high spectral resolution (Brandstetter and Lendl, 2012;Brandstetter et al, 2013). However, the absorption spectrum of urea, urea acid, creatinine, phosphate and other materials in blood or interstitial fluid overlapped with the glucose absorption spectrum (Jax et al, 2011;Martin et al, 2005), which is the biggest challenge for glucose monitoring using spectral method, especially for single or two wavelengths spectrometer.…”

Section: Specific Determination Of Glucose Using Tunable Lasermentioning

confidence: 97%

U-shaped fiber-optic ATR sensor enhanced by silver nanoparticles for continuous glucose monitoring

Shan-xin

Sun

et al. 2015

Biosensors and Bioelectronics

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“…This type of QCL was applied for analysis of complex mixtures of 2 analytes in aqueous solution in online process monitoring, 13 and for medical applications. 2,[14][15][16] High emission intensities made possible to employ larger path length for liquid transmission measurements and by that enhancing the ruggedness of the resulting analysis system compared to FTIR spectroscopy. Infrared spectroscopy is a well-established experimental technique for the secondary structure analysis of proteins.…”

mentioning

confidence: 98%

“…8 µm. 2,3 These low path lengths may considerably impair the robustness of the method due to clogging of the cell. Two decades ago, a new type of mid-IR light source, quantum cascade lasers (QCLs) were introduced.…”

mentioning

confidence: 99%

External-Cavity Quantum Cascade Laser Spectroscopy for Mid-IR Transmission Measurements of Proteins in Aqueous Solution

et al. 2015

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In this work, we report mid-IR transmission measurements of the protein amide I band in aqueous solution at large optical paths. A tunable external-cavity quantum cascade laser (EC-QCL) operated in pulsed mode at room temperature allowed one to apply a path length of up to 38 μm, which is four times larger than that applicable with conventional FT-IR spectrometers. To minimize temperature-induced variations caused by background absorption of the ν2-vibration of water (HOH-bending) overlapping with the amide I region, a highly stable temperature control unit with relative temperature stability within 0.005 °C was developed. An advanced data processing protocol was established to overcome fluctuations in the fine structure of the emission curve that are inherent to the employed EC-QCL due to its mechanical instabilities. To allow for wavenumber accuracy, a spectral calibration method has been elaborated to reference the acquired IR spectra to the absolute positions of the water vapor absorption bands. Employing this setup, characteristic spectral features of five well-studied proteins exhibiting different secondary structures could be measured at concentrations as low as 2.5 mg mL(-1). This concentration range could previously only be accessed by IR measurements in D2O. Mathematical evaluation of the spectral overlap and comparison of second derivative spectra confirm excellent agreement of the QCL transmission measurements with protein spectra acquired by FT-IR spectroscopy. This proves the potential of the applied setup to monitor secondary structure changes of proteins in aqueous solution at extended optical path lengths, which allow experiments in flow through configuration.

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Direct determination of glucose, lactate and triglycerides in blood serum by a tunable quantum cascade laser-based mid-IR sensor

Cited by 78 publications

References 27 publications

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

U-shaped fiber-optic ATR sensor enhanced by silver nanoparticles for continuous glucose monitoring

External-Cavity Quantum Cascade Laser Spectroscopy for Mid-IR Transmission Measurements of Proteins in Aqueous Solution

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