Direct determination of amino acids and carbohydrates by high-performance capillary electrophoresis with refractometric detection

Ivanov, A.R; Nazimov, Igor V.; Lobazov, A.P; Popkovich, G.B

doi:10.1016/s0021-9673(00)00705-6

Cited by 24 publications

(6 citation statements)

References 31 publications

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“…Apart from these, more frequently used detection methods, a very few papers report the use of other, less common detection methods for the CE analysis of organic acids. Thereby mostly laboratory made detection units such as detectors based on chemiluminescence [18], electrochemical principles [19], or refractometry [20] are employed.…”

Section: Choice Of Detection Modementioning

confidence: 99%

Determination of organic acids by CE and CEC methods

Klampfl

2007

Electrophoresis

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A comprehensive overview of the analysis of low-molecular-mass organic acids employing electromigration methods in the capillary format is given. This review includes papers published since 2003 and can be seen as an update of the review paper published by Galli et al. in 2003. Tables included in this review contain application papers describing the determination of organic acids from a variety of fields like the analysis of food and beverages, environmental samples, samples from clinical origin, and from natural products.

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Section: Choice Of Detection Modementioning

confidence: 99%

Determination of organic acids by CE and CEC methods

Klampfl

2007

Electrophoresis

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“…Various direct detection methods have been reported for carbohydrate combined with separation techniques, including flame ionization detection, 4 mass spectroscopy detection, 5 refractive index, 6 indirect UV (or fluorescence) 7,8 and pulsed amperometric detection 9 for gas chromatography, high performance liquid chromatography (HPLC), ion chromatography and capillary electrophoresis (CE). Because carbohydrates lack chromophores (or fluorophores) to facilitate the sensitive detection with UV (or fluorescence) detection, derivatization has most widely been used for carbohydrate analysis.…”

Section: 3mentioning

confidence: 99%

Analysis of Monosaccharides by Capillary Electrophoresis with Electrochemiluminescence Detection

Lee

2007

ANAL. SCI.

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Carbohydrates play a very important role in the human life cycle and form the base of many foodstuffs. 1 As integral components of glycoproteins and glycolipids, carbohydrates are involved in many biological processes, such as cell recognition and the immune response. 2,3 Therefore, carbohydrate analysis is still of great interest.Various direct detection methods have been reported for carbohydrate combined with separation techniques, including flame ionization detection, 4 mass spectroscopy detection, 5 refractive index, 6 indirect UV (or fluorescence) 7,8 and pulsed amperometric detection 9 for gas chromatography, high performance liquid chromatography (HPLC), ion chromatography and capillary electrophoresis (CE). Because carbohydrates lack chromophores (or fluorophores) to facilitate the sensitive detection with UV (or fluorescence) detection, derivatization has most widely been used for carbohydrate analysis. A number of methods were reported based on reductive amination with various chromophores (fluorophores): aminobenzoic acid (AA), 10 8-aminonaphthalene-1,3,6-trisulfonate (ANTS), 11 dansylhydrazine (DHZ), 12 and 9-aminoacridone (AMAC). 13 A direct chemiluminophore derivatization method was also reported using 3-aminophthalhydrazide (APH) for the analysis of monosaccharides. 14 Recently, tris(2,2′-bipyridyl)ruthenium(II) [Ru(bpy)3 3+ ]-based electrogenerated chemiluminescence (ECL) detection has been widely studied because it provides an extremely sensitive means of detection. Many works were reported for the assays of amino acids, proteins, amines, drugs with the combination of HPLC and CE separation techniques. [15][16][17][18][19][20][21] In this study, we investigate the CE-ECL method for the analysis of four monosaccharides, which usually closely positioned in CE electropherograms, using 2-diethylaminoethanethiol (DEAET) as a derivatization reagent. Experimental Reagents and chemicalsTris(2,2′-bipyridyl)dichlororuthenium (II) ) were all HPLC grade. All buffer solutions were prepared with water purified in a Milli-Q System (Millipore, Beford, MA, USA). The standard solutions of monosaccharides were prepared with methanol and stored in a refrigerator at 4˚C. ApparatusA CE system was assembled in the laboratory. A Spellman CZE 1000R (Plainview, NY, USA) was used for electrophoresis and electrokinetic injection. A 25-μm i.d. 90-cm long uncoated fused-silica capillary (Polymicro Technologies, Phoenix, AZ, USA) was used for the CE column. The detection cell used in this study was similar to that described elsewhere. 22 A Pt working electrode (1.0 mm in diameter), a Pt wire counter electrode (1 mm in diameter) and a Ag/AgCl reference electrode were used in a conventional three-electrode system. The distance from the capillary end to working electrode surface was set at 100 μm by a microcontroller. Controlled potential electrolysis for Ru(bpy)3 2+ oxidation was carried out with a BAS-Epsilon (West Lafayette, IN, USA) electrochemical analyzer. The ECL light was captured by a Hamamatsu Photonics R238 photomult...

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“…Since AAs are highly polar, nonvolatile compounds and have little chromophore, AA analysis represents one of the most challenging forms of analytical research. AAs have been commonly analyzed by chromatography and electrophoresis with pre‐ or postcolumn derivatization for UV or fluorescence detection , and underivatized AAs have been analyzed using specific detectors . Although a large number of AA analysis methodologies have been developed, most possess some drawbacks such as complex design or time‐consuming sample preparation, or inapplicability to biological samples due to lack of selectivity and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis mass spectrometry with sheathless electrospray ionization for high sensitivity analysis of underivatized amino acids

Jeong¹,

Kim²,

Park

2012

Electrophoresis

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A high durability sheathless electrospray ionization interface of CE-MS is applied for the sensitive analysis of underivatized amino acids. The sheathless interface was realized using an ionophore membrane-packed electro-conduction channel. The interface functioned well with a volatile alkaline background electrolyte (BGE) and uncoated fused-silica capillaries for CE-MS analysis of underivatized amino acids. High electroosmotic flow with alkaline BGE facilitated high separation efficiency (>100,000 theoretical plates) and short analysis time (<15 min). Both the short-term stability and long-term durability are particularly suited for routine applications. Using electrokinetic injection and the multiple reaction monitoring (MRM) mode with a triple-quadrupole analyzer, high sensitivity was achieved, which yielded detection limits of 0.05-0.81 μM. For the quantitation of underivatized amino acids, quantification precisions (RSDs) for intra- and inter-day analyses were less than 3%. Recoveries from serum were 96.3-101.8% for isotope dilution mass spectrometry (IDMS). When compared with HPLC-IDMS for human serum samples, highly agreeable (96.9-102.0%) results were obtained with the proposed CE-IDMS method.

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Direct determination of amino acids and carbohydrates by high-performance capillary electrophoresis with refractometric detection

Cited by 24 publications

References 31 publications

Determination of organic acids by CE and CEC methods

Determination of organic acids by CE and CEC methods

Analysis of Monosaccharides by Capillary Electrophoresis with Electrochemiluminescence Detection

Capillary electrophoresis mass spectrometry with sheathless electrospray ionization for high sensitivity analysis of underivatized amino acids

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