Direct detection of Δ9-tetrahydrocannabinol in saliva using a novel homogeneous competitive immunoassay with fluorescence quenching

Tan, Chongxiao; Gajovic‐Eichelmann, Nenad; Stöcklein, Walter; Polzius, R.; Bier, Frank F.

doi:10.1016/j.aca.2009.11.012

Cited by 25 publications

(15 citation statements)

References 26 publications

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“…[140] Despite its non-invasive collection, OF is a complex matrix. [144] The majority of the methods for the determination in OF of THC are based on GC-MS [126,145] and LC-MS [31,143,146,147] ; immunoassay with fluorimetric and electroanalytical detection [148,149] have been also proposed. Various collection devices have been introduced to account for this problem.…”

Section: Urinementioning

confidence: 99%

Analytical approaches for the determination of phytocannabinoids and endocannabinoids in human matrices

Battista

Sergi

Montesano

et al. 2013

Drug Testing and Analysis

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Over the last two decades, the role played by phytocannabinoids and endocannabinoids in medicine has gained increasing interest in the scientific community. Upon identification of the plant compound Δ(9)-tetrahydrocannabinol (THC) and of the endogenous substance anandamide (AEA), different methodological approaches and innovative techniques have been developed, in order to evaluate the content of these molecules in various human matrices. In this review, we discuss the analytical methods that are currently used for the identification of phytocannabinoids and endocannabinoids, and we summarize the benefits and limitations of these procedures. Moreover, we provide an overview of the main biological matrices that have been analyzed to date for qualitative detection and quantitative determination of these compounds.

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Section: Urinementioning

confidence: 99%

Analytical approaches for the determination of phytocannabinoids and endocannabinoids in human matrices

Battista

Sergi

Montesano

et al. 2013

Drug Testing and Analysis

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“…An anti-tetrahydrocannabinol antibody-based competitive immunoassay with fluorescence quenching was employed for the determination of tetrahydrocannabinol in saliva. [22] The last is theophylline, which is a methylxanthine drug used in therapy for respiratory diseases such as chronic obstructive pulmonary disease or asthma. The thermodynamic parameters of mouse anti-theophylline monoclonal antibody binding were studied using isothermal titration microcalorimetry.…”

Section: Introductionmentioning

confidence: 99%

Determination of Bovine Serum Albumin Conjugated Drugs by Immobilization as Microarrays and Oblique-Incidence Reflectivity Difference Microscopy

Sun

Lam

Zhu

2014

Instrumentation Science & Technology

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& Human serum is a mixture of various proteins which may interact with drugs. Therefore, it is of interest to investigate the binding kinetics of pharmaceuticals with their corresponding antibodies in serum. In this article, microarrays and a label-free biosensor were used to study these interactions. Microarrays provide a high-throughput platform for characterizing biomolecular interactions, and the label-free oblique-incidence reflectivity difference biosensor avoids the drawbacks of fluorescencebased methods. The experimental results show that the binding affinities between most of the drugs and their antibodies were reduced in human serum because the bulky proteins block the access to or reduce the stability of the reaction complexes. Therefore, one should be mindful when in vitro or in vivo testing the efficiency of potential drugs and their antibodies.

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“…As one of the most sensitive analytical tools, fluorescence spectroscopy has been widely used for bioanalysis. [1][2][3][4][5][6][7][8][9][10][11][12] So far, different detection approaches based on fluorescence spectroscopy have been developed for the detection of DNA, 2,4-11 one of which is the method based on fluorescence resonance energy transfer (FRET). 2,5,[7][8][9][10][11][16][17][18][19][20][21][22] Quantum dots (QDs), owing to their unique optical properties such as resistance to photobleaching, high fluorescence intensity, narrow and tunable emission and broad adsorption, [13][14][15] have attracted widespread interest, especially when used as the energy donor of the FRET system.…”

Section: Introductionmentioning

confidence: 99%

Detection of DNA based on fluorescence resonance energy transfer of polyelectrolyte-protected CdTe quantum dots as energy donors

Qiu

Zhang

et al. 2012

Analyst

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The approach for DNA detection was established by using a fluorescence resonance energy transfer (FRET) system, in which the energy donor was poly-diallyldimethylammonium chloride-protected quantum dots and the energy receptor was ethidium bromide (EB) inserting into the double stranded DNA. The concentration of the probe DNA, EB and NaCl was optimized. Under the optimized conditions, the FRET system has a stable signal and good reproducibility. The linear range is 7.7-61.6 nM with the correlation coefficient of 0.998 and the limit of detection is 7.7 nM. This method is simple and sensitive, and makes the label-free DNA detection come true.

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Direct detection of Δ9-tetrahydrocannabinol in saliva using a novel homogeneous competitive immunoassay with fluorescence quenching

Cited by 25 publications

References 26 publications

Analytical approaches for the determination of phytocannabinoids and endocannabinoids in human matrices

Analytical approaches for the determination of phytocannabinoids and endocannabinoids in human matrices

Determination of Bovine Serum Albumin Conjugated Drugs by Immobilization as Microarrays and Oblique-Incidence Reflectivity Difference Microscopy

Detection of DNA based on fluorescence resonance energy transfer of polyelectrolyte-protected CdTe quantum dots as energy donors

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