Direct detection of Strongyloides infection via molecular and antigen detection methods

Balachandra, Dinesh; Ahmad, Hussain; Arifin, Norsyahida; Noordin, Rahmah

doi:10.1007/s10096-020-03949-x

Cited by 17 publications

(10 citation statements)

References 82 publications

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“…The copyright holder for this preprint this version posted January 18, 2022. ; https://doi.org/10.1101/2022.01.18.22268971 doi: medRxiv preprint current and recent infections (11)(12)(13). In fact, antibody serology against the NIE antigen from S. stercoralis has been used to monitor MDA programs thanks to the rapid decay of antibodies after clearance of infection (14,15).…”

Section: (Which Was Not Certified By Peer Review)mentioning

confidence: 99%

“…Antibody serology could be a useful tool for detection of current infection but also exposure, which is not captured by any of the diagnostic methods above. For helminths, antibody serology has a particularly important role in the diagnosis of S. stercoralis infection, since conventional methods have a very low sensitivity and specific antibodies generally decrease 6 months after treatment, which helps detect current and recent infections (11–13). In fact, antibody serology against the NIE antigen from S. stercoralis has been used to monitor MDA programs thanks to the rapid decay of antibodies after clearance of infection (14, 15).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of antibody serology to determine current helminth andPlasmodium falciparuminfections in a co-endemic area in Southern Mozambique

Santano

Rubio

Grau-Pujol

et al. 2022

Preprint

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Background: Soil-transmitted helminths (STH), Schistosoma spp. and Plasmodium falciparum are parasites of major public health importance and co-endemic in many sub-Saharan African countries. Management of these infections requires detection and treatment of infected people and evaluation of large-scale measures implemented. Diagnostic tools are available but their low sensitivity, especially for low intensity helminth infections, leaves room for improvement. Antibody serology could be a useful approach thanks to its potential to detect both current infection and past exposure. Methodology: We evaluated total IgE responses and specific-IgG levels to 9 antigens from STH, 2 from Schistosoma spp., and 16 from P. falciparum, as potential markers of current infection in a population of children and adults from Southern Mozambique (N = 715). Antibody responses were measured by quantitative suspension array Luminex technology and their performance was evaluated by ROC curve analysis using microscopic and molecular detection of infections as reference. Principal findings: IgG against the combination of EXP1, AMA1 and MSP2 (P. falciparum) in children and NIE (Strongyloides stercoralis) in adults and children had the highest accuracies (AUC = 0.942 and AUC = 0.872, respectively) as markers of current infection. IgG against the combination of MEA and Sm25 (Schistosoma spp.) were also reliable markers of current infection (AUC = 0.779). In addition, IgG seropositivity against 20 out of the 27 antigens in the panel differentiated the seropositive endemic population from the non-endemic population, suggesting a possible role as markers of exposure. Conclusions: We provided evidence for the utility of antibody serology to detect current infection with parasites causing tropical diseases in endemic populations. In addition, most of the markers could be used as markers of exposure. We also showed the feasibility of measuring antibody serology with a platform that allows the integration of control and elimination programs for different pathogens.

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Section: (Which Was Not Certified By Peer Review)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of antibody serology to determine current helminth andPlasmodium falciparuminfections in a co-endemic area in Southern Mozambique

Santano

Rubio

Grau-Pujol

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…For helminths, antibody serology has a particularly important role in the diagnosis of S . stercoralis infection, since conventional methods have a very low sensitivity and specific antibodies generally decrease 6 months after treatment, which helps detect current and recent infections [ 11 – 13 ]. In fact, antibody serology against the NIE antigen from S .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of antibody serology to determine current helminth and Plasmodium falciparum infections in a co-endemic area in Southern Mozambique

et al. 2022

View full text Add to dashboard Cite

Background Soil-transmitted helminths (STH), Schistosoma spp. and Plasmodium falciparum are parasites of major public health importance and co-endemic in many sub-Saharan African countries. Management of these infections requires detection and treatment of infected people and evaluation of large-scale measures implemented. Diagnostic tools are available but their low sensitivity, especially for low intensity helminth infections, leaves room for improvement. Antibody serology could be a useful approach thanks to its potential to detect both current infection and past exposure. Methodology We evaluated total IgE responses and specific-IgG levels to 9 antigens from STH, 2 from Schistosoma spp., and 16 from P. falciparum, as potential markers of current infection in a population of children and adults from Southern Mozambique (N = 715). Antibody responses were measured by quantitative suspension array Luminex technology and their performance was evaluated by ROC curve analysis using microscopic and molecular detection of infections as reference. Principal findings IgG against the combination of EXP1, AMA1 and MSP2 (P. falciparum) in children and NIE (Strongyloides stercoralis) in adults and children had the highest accuracies (AUC = 0.942 and AUC = 0.872, respectively) as markers of current infection. IgG against the combination of MEA and Sm25 (Schistosoma spp.) were also reliable markers of current infection (AUC = 0.779). In addition, IgG seropositivity against 20 out of the 27 antigens in the panel differentiated the seropositive endemic population from the non-endemic population, suggesting a possible role as markers of exposure although sensitivity could not be assessed. Conclusions We provided evidence for the utility of antibody serology to detect current infection with parasites causing tropical diseases in endemic populations. In addition, most of the markers have potential good specificity as markers of exposure. We also showed the feasibility of measuring antibody serology with a platform that allows the integration of control and elimination programs for different pathogens.

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“…The S. stercoralis life cycle is complex and includes direct, indirect, and auto-infective ones 4 . Regarding the property of the auto-infective cycle, the parasite remains undiagnosed for years and causes a chronic disease that resists treatment 5 . Nearly, half of the patients with strongyloidiasis are asymptomatic, which results in the development of a chronic disease due to the unique life cycle of the parasite 2 .…”

Section: Introductionmentioning

confidence: 99%

“…Diagnostic methods for strongyloidiasis can fall into two main categories of serological and non-serological approaches, and often a mixture of both methods is required for accurate and proper diagnosis. Non-serological systems are based on intestinal parasite recognition, molecular methods, and antigen detection, while the serological approaches are based on the finding of anti- S. stercoralis antibodies 5 , 7 .…”

Section: Introductionmentioning

confidence: 99%

Designing a Multi-Epitope Antigen for Serodiagnosis of Strongyloides stercoralis Based on L3Nie.01 and IgG Immunoreactive Epitopes

Movahedpour¹,

Mostafavi‐Pour²,

Sarkari³

et al. 2022

AJMB

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Background: Serological diagnosis of Strongyloides stercoralis (S. stercoralis) is frequently challenging because of cross-reactivity with other parasitic nematodes. Therefore, it is necessary to introduce novel serological tests with high performance to properly diagnose this neglected parasitic infection. The purpose of the current study was to design a multi-epitope construct for the diagnosis of S. stercoralis. Methods: For the purpose of this study, first, highly antigenic segments and potential immunodominant epitopes of S. stercoralis were identified from two antigenic proteins, and then all of the selected parts were linked by an appropriate linker. Next, the physico-chemical features of the designed construct were analyzed. Then, tertiary structures of the construct were built and evaluated to find out the best one. Lastly, the amino acid sequence was reverse-translated and optimized for over-expression in Escherchia coli (E. coli). Results: The bioinformatic evaluation indicated that the designed protein construct could be hydrophilic, thermostable, and acidic and the estimated half-life was more than 10 hr in E. coli. Conclusion: According to the results of the study, the designed construct could be used as an efficient antigen in the ELISA system for serological diagnosis of human strongyloidiasis.

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Direct detection of Strongyloides infection via molecular and antigen detection methods

Cited by 17 publications

References 82 publications

Evaluation of antibody serology to determine current helminth andPlasmodium falciparuminfections in a co-endemic area in Southern Mozambique

Evaluation of antibody serology to determine current helminth andPlasmodium falciparuminfections in a co-endemic area in Southern Mozambique

Evaluation of antibody serology to determine current helminth and Plasmodium falciparum infections in a co-endemic area in Southern Mozambique

Designing a Multi-Epitope Antigen for Serodiagnosis of Strongyloides stercoralis Based on L3Nie.01 and IgG Immunoreactive Epitopes

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