Aims: The aim of this study was to compare the real‐time iQ‐CheckTMSalmonella kit (Bio‐Rad) with the immunocapture assay RapidCheck®Salmonella method, and a conventional culture method (FSIS, USDA) in detecting Salmonella in naturally contaminated turkey meat products. This study was also designed to determine if a selective enrichment step might improve the real‐time detection of Salmonella.
Methods and Results: Using the culture method, Salmonella was recovered from 49 out of 99 retail turkey meat samples collected. RapidCheck® failed to detect 11 Salmonella samples that were positive by the culture method. The iQ‐CheckTM real‐time PCR also failed to detect three samples that were positive by the culture method. However, when carried out after a selective enrichment step, the iQ‐CheckTM real‐time PCR detected all 49 Salmonella samples recovered by the culture method. The iQ‐CheckTM real‐time PCR detected the presence of Salmonella in some samples that were not recovered by the culture method.
Conclusions: Adding a selective enrichment step to the iQ‐CheckTM real‐time PCR improves the detection of Salmonella in naturally contaminated turkey meat samples.
Significance and Impact of the Study: The iQ‐CheckTMSalmonella real‐time PCR can be used as a rapid method to monitor Salmonella in turkey meat, together with conventional culture methodology.