Direct Detection of Red Blood Cell Fragments

Linneweber, Joerg; Chow, Tom; Takano, T.; Maeda, Takeshi; Nonaka, Kenji; Schulte-Eistrup, S.; Kawahito, S.; Moake, JL; Nosé, Y.

doi:10.1097/00002480-200003000-00126

Cited by 1 publication

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“…By contrast, linear peristaltic pumps have been found to increase the free hemoglobin concentration in other studies 11 . Linneweber et al 5 reported an increase in the quantity of RBC fragments when a roller pump is used in comparison with a centrifugal pump. As many of these studies only assessed in vitro parameters, or only involved humans undergoing cardiopulmonary bypass, the clinical significance in dogs is unknown.…”

Section: Introductionmentioning

confidence: 87%

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Influence of transfusion technique on survival of autologous red blood cells in the dog

McDevitt

Ruaux

Baltzer

2011

J Vet Emergen Crit Care

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Objective To determine the effect of 3 differing transfusion techniques on survival of autologous canine red blood cells (RBCs). Design Prospective, blinded study. Setting University Teaching Hospital. Animals Nine healthy dogs. Interventions Three distinct preparations of RBCs, each representing ∼1% of red cell mass, were generated for each dog by biotinylation of RBCs at varying biotin densities. Labeled cells were transfused using 3 techniques (gravity, volumetric pump, syringe pump). Serial determinations of red cell survival were carried out by flow-cytometric analysis of RBCs collected at 7-day intervals for 49 days. In vitro analysis of the effect of transfusion methods on RBC integrity and osmotic fragility were carried out in 7/9 dogs. Measurements and Main Results RBCs administered via volumetric and syringe pumps exhibited a marked decrease in short-term probability of survival compared to RBCs delivered by gravity flow. At 24 hours, only 4/8 and 1/7 dogs had surviving cell populations delivered by volumetric and syringe pump respectively, compared with 8/8 dogs which had surviving cell populations delivered by gravity flow. Circulating half-life of cells surviving at 24 hours after delivery by volumetric pump was not significantly different to that delivered by gravity flow . No significant effect on in vitro RBC integrity or osmotic fragility was detected in relation to transfusion technique. Conclusions Delivery of autologous canine RBCs via mechanical delivery systems was associated with a high risk for early loss of transfused cells.

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Section: Introductionmentioning

confidence: 87%

“…A number of studies are available in the human literature that compare peristaltic pumps to centrifugal pumps or other methods of administration 5–9 . Most of these studies assess damage to red cells in vitro, and rarely examine the effect that transfusion administration has on in vivo red cell survival.…”

Section: Introductionmentioning

confidence: 99%