Direct detection of Pseudomonas aeruginosa from patients with healthcare associated pneumonia by real time PCR

Feizabadi, Mohammad Mehdi; Majnooni, Araz; Nomanpour, Bizhan; Fatolahzadeh, Bahram; Raji, Nafiseh; Delfani, Somayeh; Habibi, Minoo; Asadi, Sorour; Parvin, Mahmood

doi:10.1016/j.meegid.2010.08.008

Cited by 36 publications

(31 citation statements)

References 18 publications

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“…Treatment of these infections is difficult, as they show high levels of intrinsic or acquired resistance to different antimicrobial agents, markedly reducing the antibiotic options available for treatment 4,5 . Bacterial species isolated from cases with respiratory tract infections usually show strong resistance to antimicrobial agents; this could be related to the potential for biofi lm formation in respiratory tract.…”

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confidence: 99%

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Synergistic interactions in mixed-species biofilms of pathogenic bacteria from the respiratory tract

Varposhti

Entezari

Feizabadi

2014

Rev. Soc. Bras. Med. Trop.

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Introduction: Mixed-species biofi lms are involved in a wide variety of infections. We studied the synergistic interactions during dual-species biofi lm formation among isolates of Pseudomonas aeruginosa, Acinetobacter baumannii, and Stenotrophomonas maltophilia. Methods: Isolates were cultured as single-species and all possible combinations of dual-species biofi lms. Results: The 61 A. baumannii biofi lms increased by 26-fold when cultured with S. maltophilia isolates; 62 A. baumannii biofi lms increased by 20-fold when cultured with S. maltophilia isolates; and 31 P. aeruginosa biofi lms increased by 102-fold when cultured with S. maltophilia 106. Conclusions: Synergy was observed between two isolates, including those that inherently lacked biofi lm formation ability.

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confidence: 99%

“…Polymicrobial infections have been observed in such patients 4 . The aim of this study was to evaluate the occurrence of synergistic interactions during dual-species biofi lm formation by the bacteria isolated from respiratory tract of these patients.…”

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confidence: 99%

Synergistic interactions in mixed-species biofilms of pathogenic bacteria from the respiratory tract

Varposhti

Entezari

Feizabadi

2014

Rev. Soc. Bras. Med. Trop.

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“…Feizebaldi [131] have shown previously that a TaqMan assay designed to amplify a 117 base pair region of this gene is specific to P. aeruginosa.…”

Section: P Aeruginosa Specific Pcrmentioning

confidence: 99%

“…The fluorescent reporter used in this project was FAM. TaqMan primers [131] directed at the oprL gene were used in this study to detect and quantify P. aeruginosa in sputum.…”

Section: Taqman Optimisationmentioning

confidence: 99%

“…P. aeruginosa levels were determined using a TaqMan assay, in which a 117 bp region between positions 330 to 447 of the P. aeruginosa oprL gene was amplified, as described previously [131]. H. influenzae density was determined using a TaqMan assay, in which a 90-bp region between positions 518 to 608 of the H. influenzae hel gene was amplified [10].…”

Section: Quantitative Pcr For the Determination Of Bacterial Loadmentioning

confidence: 99%

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An investigation into the effect of erythromycin on Pseudomonas aeruginosa quorum sensing in non-cystic fibrosis bronchiectasis

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Antibiotic Resistance Detection in Pseudomonas aeruginosa: Recent Strategies, Advances, and Challenges

Clarindo Lopes,

Koushanpour,

Wiebe

et al. 2024

Analysis & Sensing

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Antibiotic resistance presents a worldwide public health emergency, impeding the effective management of infectious diseases. Pseudomonas aeruginosa plays a substantial role as a bacterial pathogen, particularly in infections among hospitalized individuals, and those with weakened immune systems. Timely and accurate detection of antimicrobial resistance in P. aeruginosa is crucial for initiating tailored antibiotic therapy promptly, thus improving patient outcomes. Nevertheless, this endeavor encounters challenges due to the intricate and varied nature of antibiotic‐resistant strains. Extensive efforts have been invested in developing sensors and instrumentations for assessing antibiotic resistance or susceptibility (AST), aiming to enable personalized patient treatment with appropriate medications. This paper focuses on recent advancements in these methodologies, utilized for evaluating the degree of antibiotic resistance or susceptibility inpathogenic bacteria. Flow cytometry, dual molecular recognition and mass spectrometry are presented as newer phenotypic AST techniques. Genomic methods and the pyocyanin detection are listed as methodologies for the detection of resistance indicators.

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Direct detection of Pseudomonas aeruginosa from patients with healthcare associated pneumonia by real time PCR

Cited by 36 publications

References 18 publications

Synergistic interactions in mixed-species biofilms of pathogenic bacteria from the respiratory tract

Synergistic interactions in mixed-species biofilms of pathogenic bacteria from the respiratory tract

An investigation into the effect of erythromycin on Pseudomonas aeruginosa quorum sensing in non-cystic fibrosis bronchiectasis

Antibiotic Resistance Detection in Pseudomonas aeruginosa: Recent Strategies, Advances, and Challenges

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