Direct detection of Helicobacter pylori from biopsies of patients in Lagos, Nigeria using real-time PCR—a pilot study

Ajayi, Abraham; Jolaiya, Tolu; Smith, Stella Ifeanyi

doi:10.1186/s13104-021-05505-y

Cited by 6 publications

(3 citation statements)

References 14 publications

(10 reference statements)

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“…Results revealed that PCR using the glmM gene was also a reliable test compared to histology and the CLO test. Other studies Smith et al [103], Onyekere et al [77], Smith et al [78], Palamides et al [16], and Ajayi et al [79] used SAT, UBT, stool-PCR, UBT and culture from biopsy, and qPCR, respectively, in the diagnosis of HPI in Nigeria. The use of endoscopy, histology, and culture in the diagnosis of HPI has also been reported in Senegal by Breurec et al [80]; Seck et al [81]; and Doh et al [82].…”

Section: Diagnosis Of Hpi In Africamentioning

confidence: 99%

Helicobacter pylori Infection in Africa: Update of the Current Situation and Challenges

Smith¹,

Ajayi²,

Jolaiya³

et al. 2021

Dig Dis

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Background: The burden of Helicobacter pylori infection (HPI) in Africa remains high with varying levels of prevalence among children and adults reported in different regions of the continent. Persistent and uneradicated HPI could result in gastric cancer, although less severe pathological outcomes have been reported among Africans – the so-called “African enigma.” Summary: Analysis of endoscopic findings of the upper gastrointestinal tract demonstrates similarities with that of patients from the West. Thus, it could be asserted that the true picture of HPI in Africa is yet to be unveiled due to several challenges including inadequate health-care system, lack of treatment guidelines and standardized protocol for diagnosis, and lack of data. This review explores the prevalence, diagnosis, treatment, and health-care system in Africa as it relates to HPI, thus providing an update and highlighting the need for an African HPI guideline. Key Messages: There is high prevalence of Helicobacter pylori infection (HPI) in Africa with an increasing burden of antibiotic resistance. Various methods including invasive and noninvasive methods are deployed in the diagnosis of HPI in Africa. There is a need for consensus on diagnosis and treatment of HPI in Africa.

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Section: Diagnosis Of Hpi In Africamentioning

confidence: 99%

Helicobacter pylori Infection in Africa: Update of the Current Situation and Challenges

Smith¹,

Ajayi²,

Jolaiya³

et al. 2021

Dig Dis

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“…Limitations include costs and time constraints but microbial culture also enables phenotypical antimicrobial susceptibility testing (AST) 180 . Molecular testing from formalin-embedded biopsy or RUT samples with PCR methods, of which quantitative PCR is most appropriate, and fluorescence in situ hybridization is highly accurate in the detection of H. pylori 185 and can also be combined with molecular resistance testing 186 .…”

Section: Primermentioning

confidence: 99%

Helicobacter pylori infection

Malfertheiner

Camargo

El-Omar

et al. 2023

Nat Rev Dis Primers

237

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Helicobacter pylori infection causes chronic gastritis, which can progress to severe gastroduodenal pathologies, including peptic ulcer, gastric cancer and gastric mucosa-associated lymphoid tissue lymphoma. H. pylori is usually transmitted in childhood and persists for life if untreated. The infection affects around half of the population in the world but prevalence varies according to location and sanitation standards. H. pylori has unique properties to colonize gastric epithelium in an acidic environment. The pathophysiology of H. pylori infection is dependent on complex bacterial virulence mechanisms and their interaction with the host immune system and environmental factors, resulting in distinct gastritis phenotypes that determine possible progression to different gastroduodenal pathologies. The causative role of H. pylori infection in gastric cancer development presents the opportunity for preventive screen-and-treat strategies. Invasive, endoscopy-based and non-invasive methods, including breath, stool and serological tests, are used in the diagnosis of H. pylori infection. Their use depends on the specific individual patient history and local availability. H. pylori treatment consists of a strong acid suppressant in various combinations with antibiotics and/or bismuth. The dramatic increase in resistance to key antibiotics used in H. pylori eradication demands antibiotic susceptibility testing, surveillance of resistance and antibiotic stewardship.

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“…Stool PCR and direct detection of H. pylori in biopsies using real-time PCR have been demonstrated in Nigeria [96]. Droplet digital PCR (ddPCR) is more sensitive than other PCR methods.…”

Section: Wg 1: Epidemiologymentioning

confidence: 99%

Helicobacter pylori Diagnosis and Treatment in Africa: The First Lagos Consensus Statement of the African Helicobacter and Microbiota Study Group

Smith,

Schulz,

Ugiagbe

et al. 2024

Dig Dis

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Background: Helicobacter pylori (H. pylori) infection is the most prevalent type of bacterial infection. Current guidelines from different regions of the world neglect specific African conditions and requirements. The African Helicobacter and Microbiota Study Group (AHMSG), founded in 2022, aimed to create an Africa-specific consensus report reflecting Africa-specific issues. Summary: Eighteen experts from nine African countries and two European delegates supported by nine African collaborators from eight other countries prepared statements on the most important African issues in four working groups: (1) epidemiology, (2) diagnosis, (3) indications and prevention, and (4) treatment. Limited resources, restricted access to medical systems, and underdeveloped diagnostic facilities differ from those of other regions. The results of the individual working groups were presented for the final consensus voting, which included all board members. Key Messages: There is a need for further studies on H. pylori prevalence in Africa, with diagnosis hinged on specific African situation. Treatment of H. pylori in the African setting should be based on accessibility and reimbursement, while indication and prevention should be defined in specific African countries.

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Direct detection of Helicobacter pylori from biopsies of patients in Lagos, Nigeria using real-time PCR—a pilot study

Cited by 6 publications

References 14 publications

<b><i>Helicobacter pylori</i></b> Infection in Africa: Update of the Current Situation and Challenges

<b><i>Helicobacter pylori</i></b> Infection in Africa: Update of the Current Situation and Challenges

Helicobacter pylori infection

<i>Helicobacter pylori</i> Diagnosis and Treatment in Africa: The First Lagos Consensus Statement of the African Helicobacter and Microbiota Study Group

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