Direct Detection of DNA and DNA‐Ligand Interaction by Impedance Spectroscopy

Witte, Christian; Lisdat, Fred

doi:10.1002/elan.201000410

Cited by 27 publications

(17 citation statements)

References 45 publications

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“…Electrochemical impedance spectroscopy (EIS) is a technique that can be utilised to observe biorecognition events on functionalised electrode surfaces. (Ciani et al, ; Corrigan et al, ; Corrigan et al, ; Gebala et al, ; Huang et al, ; Kafka et al, ; Lisdat and Schäfer, ; Witte and Lisdat, ) The detection of DNA hybridisation events using EIS is a growing field of study and has been covered extensively in review articles. (Cederquist and Kelley, ; Lindholm‐Sethson et al, ; Lisdat and Schäfer, ; Park and Park, ; Suginta et al, ) In general, nucleic acid probes are functionalised onto an electrode surface, which has been so far mainly, if not exclusively, a gold electrode surface, forming a mixed self‐assembled monolayer (SAM).…”

Section: Electrochemical Impedance Spectroscopy and Electrochemical Smentioning

confidence: 99%

Carbon screen‐printed electrodes on ceramic substrates for label‐free molecular detection of antibiotic resistance

Obaje

Cummins

Schulze

et al. 2016

J of Interdiscip Nanomed

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The growing threat posed by antimicrobial resistance on the healthcare and economic well‐being of mankind is pushing the need to develop novel and improved diagnostic platforms for its rapid detection at point of care, facilitating better patient management strategies during antibiotic therapy. In this paper, we present the manufacturing and characterisation of a low‐cost carbon screen‐printed electrochemical sensor on a ceramic substrate. Using label‐free electrochemical impedance spectroscopy, the sensor is demonstrated for the detection of blaNDM, which is one of the main antimicrobial resistance factors in carbapenem‐resistant Enterobacteriaceae. The electrochemical performance of the newly fabricated sensor was initially investigated in relation to the function of its underlying composite materials, evaluating the choice of carbon and dielectric pastes by characterising properties like surface roughness, wetting and susceptibility of unspecific DNA binding. Subsequently, the sensor was used in an electrochemical impedance spectroscopy assay for the sensitive and specific detection of synthetic blaNDM targets achieving a detection limit of 200 nM. The sensor properties and performance demonstrated in this study proved the suitability of the new electrode materials and manufacturing for further point‐of‐care test development as an inexpensive and effective alternative to gold electrodes sensor.

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Section: Electrochemical Impedance Spectroscopy and Electrochemical Smentioning

confidence: 99%

Carbon screen‐printed electrodes on ceramic substrates for label‐free molecular detection of antibiotic resistance

Obaje

Cummins

Schulze

et al. 2016

J of Interdiscip Nanomed

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“…Indirect labels like intercalating molecules can offer a useful compromise between easier sample preparation and improved detection sensitivity, whereby direct labelling of the target nucleic acid sequence is not required. have also been used in conjunction with EIS to serve as signal validation tools to exclude potential false positives by non-specific adsorption [20,21]. These molecules cause a decrease in R ct upon binding to dsDNA, but cause little or no change with ssDNA.…”

Section: Introductionmentioning

confidence: 99%

A novel cobalt complex for enhancing amperometric and impedimetric DNA detection

Regan¹,

Hallett²,

Wong³

et al. 2014

Electrochimica Acta

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In this work we present a novel cobalt complex, [Co(GA) 2 (aqphen)]Cl that is water-soluble, redox-active and binds to dsDNA. We report that this complex can be used as a signal enhancer when detecting DNA hybridisation using electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The compound mediates its EIS signal enhancement by causing an increase in charge transfer resistance (R ct ) when bound to dsDNA. Increased peak currents are also observed with DPV when the compound is incubated with dsDNA as compared with ssDNA. We believe that this compound intercalates specifically with dsDNA and alters the DNA structure to affect the electrostatic barrier to charged redox markers in solution. To our knowledge this is the first example of a single compound that can enhance both amperometric and impedimetric signals for DNA detection.Our findings enable the development of a label-free and multi-modal approach to improve the sensitivity, accuracy and speed of electrochemical DNA detection.

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“…It is well known that the presence of negatively charged oligonucleotides on an electrode causes a decrease in the rate of interfacial electron transfer for the [Fe(CN) 6 ] 4À/3À redox couple [8]. The effect can be quantitatively determined using EIS when spectra are fitted by a simple equivalent circuit (insert in Figure 1), which consists of electron-transfer resistance (R ET ), a constant phase element (CPE), a Warburg element (Z W ) and the resistance of the solution (R S ).…”

mentioning

confidence: 99%

Label‐Free Impedance Aptasensor for Major Peanut Allergen Ara h 1

Trashin¹,

Jong²,

Breugelmans³

et al. 2014

Electroanalysis

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[a] Aptamers (synthetic oligonucleotides with an affinity to a given target) hold great potential as bio-recognition elements in biosensors [1]. Selected through the systematic evolution of ligands by exponential enrichment (SELEX) process [2] from a random DNA library, aptamers have shown good affinity to all kinds of analytes: drugs, toxins, proteins, low-molecular metabolites, and so forth [3].Although aptamers and their targets are generally redox inactive, label-free electrochemical detection is possible when a soluble redox indicator is present in the measuring solution.[4] Particularly, this strategy has been successfully applied for the detection of thrombin using the 15-base thrombin-binding aptamer (TBA) [5] and to detect immunoglobulin E (IgE) using a 37-base aptamer [6]. The detection principle states that bulky proteins insulate the electrode surface, owing to capturing by the immobilized aptamer. This results in a decreased electrontransfer rate between a soluble redox indicator and an electrode. It is clear that the insulating effect will be noticeable when the target is relatively large compared to the aptamer. For instance, thrombin (MW 37 000) has a molecular weight about eight times greater than TBA (MW 4700). But such a small aptamer is rather unique. Binding domains of recently developed aptamers are unknown, that forces to use a full SELEX sequence, which normally includes two primer regions of 20 bases and a variable sequence of about 40 bases. In the present work, we challenge electrochemical detection of a major peanut allergen Ara h 1 that appears to be an appropriate bulky target for a recently developed 80-base aptamer [7].Ara h 1 is a stable trimeric protein (about eight times larger than its aptamer) which belongs to vicilin family of seed storage globulins found in nuts, seeds, and soy. These foods are listed as major food allergens and must be indicated on packages, according to European Direc- Simplicity of electrochemical sensing, along with ease of aptamer production and immobilization, allow feature elaboration of simple, cheap, and miniaturized sensing devices, which offer great possibilities in food quality control.To prepare electrodes sensitive to Ara h 1, the gold electrodes were modified by the aptamer through the thiol functional group and treated with 2-mercaptoethanol (ME) as a blocking agent. It is well known that the presence of negatively charged oligonucleotides on an electrode causes a decrease in the rate of interfacial electron transfer for the [Fe(CN) 6 ] 4À/3À redox couple [8]. The effect can be quantitatively determined using EIS when spectra are fitted by a simple equivalent circuit (insert in Figure 1), which consists of electron-transfer resistance (R ET ), a constant phase element (CPE), a Warburg element (Z W ) and the resistance of the solution (R S ). The parameter R ET provides necessary information about electron-transfer kinetics and can be visually estimated from EIS in a complex plane diagram (Nyquist plot) as the diameter of the distorted s...

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Direct Detection of DNA and DNA‐Ligand Interaction by Impedance Spectroscopy

Cited by 27 publications

References 45 publications

Carbon screen‐printed electrodes on ceramic substrates for label‐free molecular detection of antibiotic resistance

Carbon screen‐printed electrodes on ceramic substrates for label‐free molecular detection of antibiotic resistance

A novel cobalt complex for enhancing amperometric and impedimetric DNA detection

Label‐Free Impedance Aptasensor for Major Peanut Allergen Ara h 1

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