Direct detection, capsular typing and β-lactamase resistance genes in Haemophilus influenzae isolated from sinusitis samples

Abstract

Objective: Haemophilus influenzae is the causative agent of invasive and noninvasive infections such as chronic obstructive lung disease exacerbation, sinusitis, otitis media, and conjunctivitis. The study was undertaken to direct detection, capsular typing, and investigate the occurrence of β-lactamase resistance genes in H. influenzae strains isolated from sinusitis samples. Materials and methods: We investigated the presence of H influenzae in 137 sinusitis samples obtained from the patients had referred to Rasoole-Akram Hospital in Tehran in 2017. DNA extraction was performed according to the QIAamp kit. polymerase chain reaction (PCR) amplification was performed with specific primers to determine H. influenzae and capsular-typing and to detect resistance genes. Results: Eleven samples were positive for the presence of H. influenzae. Overall, 5, 2, and 4 isolates were H. influenzae type b, type A, and nontypeable H. influenzae, respectively. Of 11 isolates of H. influenzae, only two isolates (18%) were β-lactamase positive and carried the TEM-1 gene, but others were negative for the ROB-1 gene. As well, the current study showed that 45.5% (n; five of 11 encapsulated H. influenzae) of isolates were type b, which were the predominant types. Conclusion: Our data suggest that the conventional PCR can help increase detection rates of bacterial cause of sinusitis and resistance genes in clinical samples for effective treatment in individual sensitive to invasive infection.

Antimicrobial Resistance Pattern of Moraxella catarrhalis and Haemophilus influenza in Iran; a Systematic review

“…One of the studies investigated the genes involved in antibiotic resistance in H. in uenzae and identi ed the TEM1 gene in 2 out of 11 samples (18.2%) expressing β-lactamase. This study did not nd any samples with ROB1 gene (0/11) [21].…”

Antimicrobial Resistance Pattern of Moraxella catarrhalis and Haemophilus influenza in Iran; a Systematic Review

“…All original studies that were not reviews, case reports, or letters Letters, case reports, case series, reviews, systematic reviews, meta-analysis studies in Iran were analyzed to ensure that there were no missing relevant articles, but none were found. Eventually, nine articles were included in this study, of which five studies were about H. influenzae [8][9][10][11][12] , and three studies were about M. catarrhalis antibiotic resistance pattern [13][14][15] . One article was about antibiotic resistance of both H. influenzae and M. catarrhalis [16] .…”

“…These samples were isolated from nasopharynx (n=73), sinus (n=11), nasolacrimal duct discharge (n=11), sputum (n=7), adenoid tissue (n=6), and cerebrospinal fluid (n=3). All six studies were cross-sectional and used the disc diffusion method (CLSI) for antibiotic resistance testing, except for the study by Eshaghi et al (2019) [9] , which used the polymerasechange reaction (PCR) to detect ROB- (resistant) were associated with 96, 2, and 2% of the isolates, respectively [8] . On the other hand, two isolates from sinus samples were positive for TEM-1 gene, which indicates resistance to β-lactams [9] .…”

“…All six studies were cross-sectional and used the disc diffusion method (CLSI) for antibiotic resistance testing, except for the study by Eshaghi et al (2019) [9] , which used the polymerasechange reaction (PCR) to detect ROB- (resistant) were associated with 96, 2, and 2% of the isolates, respectively [8] . On the other hand, two isolates from sinus samples were positive for TEM-1 gene, which indicates resistance to β-lactams [9] . Isolates from nasolacrimal duct discharge showed the highest sensitivity to ciprofloxacin and vancomycin [10] .…”

“…Changing epidemiology of Haemophilus influenzae in children... [5] Bartlett JG, Dowell SF, Mandell LA, File Jr TM, Musher DM, Fine MJ. Practice guidelines for the management of community-acquired... [6] Page MJ, McKenzie JE, Bossuyt PM, Boutron I, Hoffmann TC, Mulrow CD, et al The PRISMA 2020 statement: An updated... [7] Ouzzani M, Hammady H, Fedorowicz Z, Elmagarmid A. Rayyan: A web and mobile app for systematic reviews... [8] Shooraj F, Mirzaei B, Mousavi SF, Hosseini F. Clonal diversity of Haemophilus influenzae carriage... [9] Eshaghi H, et al Direct detection, capsular typing, and β-lactamase... [10] Eslami F, Ghasemi Basir HR, Moradi A, Heidari Farah S. Microbiological study of dacryocystitis in northwest of Iran... [11] Farajzadeh Sheikh A, Rahimi R, Meghdadi H, Alami A, Saki M. Multiplex polymerase chain reaction detection of Streptococcus... [12] Hadi N, Bagheri K. A five-year retrospective multicenter study on etiology and antibiotic resistance pattern of... [13] Eghbali M, Baserisalehi M, Ghane M. Isolation, identification, and antibacterial susceptibility testing of Moraxella catarrhalis... [14] Mohammad Shafiei P, Baserisalehi M, Mobasherizade S. Investigating the antibiotic resistance prevalence and... [15] Nouri F, et al Prevalence of common nosocomial infections and... [16] Sabz G, Moradi S, Sharifi A, Naghmachi M, Sisakht MT, Khoramrooz SS. Identification and detection of pathogenic... [17] Vaez H, Sahebkar A, Pourfarzi F, Yousefi-Avarvand A, Khademi F. Prevalence of antibiotic resistance of... [18] Kılıç H, Akyol S, Parkan Ö� M, Dinç G, Sav H, Aydemir G. Molecular characterization and antibiotic susceptibility of Haemophilus influenzae... [19] Mather MW, Drinnan M, Perry JD, Powell S, Wilson JA, Powell J.…”