Direct Detection and Discrimination of Nucleotide Polymorphisms Using Anthraquinone Labeled DNA Probes

Goodchild, Sarah A.; Gao, Rachel; Shenton, Daniel P.; McIntosh, Alastair J. S.; Brown, Tom; Bartlett, Philip N.

doi:10.3389/fchem.2020.00381

Cited by 6 publications

(2 citation statements)

References 72 publications

(104 reference statements)

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“… 41 The targeted analytes could be cancer biomarkers, 41 viruses, 42 cardiac biomarkers, 43,44 glucose, 45 hydrogen peroxide (H 2 O 2 ), 46 foodborne pathogens 47 and heavy metal ions. 48 The common electrode substrates used to construct the immobilised biomolecule probes are GC electrode, 49 gold electrode, 50,51 screen-printed carbon electrode (SPCE) 52,53 and indium tin oxide (ITO) electrode. 45,54 To the best of our knowledge, only a few articles have been initiated in reporting the accessibility of the conductive thermoplastic carbon nanomaterial 3D-printed electrodes as electrode platforms for electrochemical biosensing purposes.…”

Section: Polymer/carbon Nanomaterials 3d-printed Electrodes For Elect...mentioning

confidence: 99%

Recent progress of conductive 3D-printed electrodes based upon polymers/carbon nanomaterials using a fused deposition modelling (FDM) method as emerging electrochemical sensing devices

et al. 2021

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Section: Polymer/carbon Nanomaterials 3d-printed Electrodes For Elect...mentioning

confidence: 99%

Recent progress of conductive 3D-printed electrodes based upon polymers/carbon nanomaterials using a fused deposition modelling (FDM) method as emerging electrochemical sensing devices

et al. 2021

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“…Thus, electrochemical nucleic acid-based sensors are well suited for point-of-care DNA polymorphism analysis . While some electrochemical nucleic acid-based sensors with rationally designed architectures can offer single-step, room-temperature polymorphism detection directly in complex samples, − most of these electrochemical nucleic acid-based sensors usually do not possess the high sensitivity required for detecting DNA polymorphisms in unamplified biological samples. To date, there is no single electrochemical nucleic acid-based sensor that can detect the three major polymorphisms: SNPs, indel polymorphisms, and DNA methylation.…”

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confidence: 99%

Use of Electrocatalysis for Differentiating DNA Polymorphisms and Enhancing the Sensitivity of Electrochemical Nucleic Acid-Based Sensors with Covalent Redox Tags—Part II

Wu¹,

Ali²,

White³

2020

ACS Sens.

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Single-nucleotide polymorphisms (SNPs), insertion/deletion (indel) polymorphisms, and DNA methylation are the most frequent types of genetic variations. As such, DNA polymorphisms play significant roles in genetic mapping and diagnostics. Thus, analytical methods enabling DNA polymorphism detection will provide an invaluable means for early disease diagnosis. However, no single electrochemical nucleic acid-based sensor has achieved the detection of the three major polymorphisms (SNPs, indel polymorphisms, and DNA methylation) with sufficient specificity and sensitivity. In response, we explore the utilization of a catalytic reaction between methylene blue (MB) covalently linked to surface-bound nucleic acid and freely diffusing ferricyanide (Fe(CN)6 3–) to improve specificity and sensitivity of DNA polymorphism detection. We find that the dynamics of the nucleic acid tether is an additional rate-limiting factor for the electrocatalytic reaction, in addition to the more traditional kinetic and excess factors. Our proof-of-concept experiments demonstrate that the use of electrocatalysis enables differentiation of the three polymorphisms when target sequences are present at 10 nM. We hypothesize that this ability is a result of the distinct dynamics of the DNA probe with each respective polymorphism. In addition to the specificity the sensor displays, the sensor achieves a 20 pM limit of detection. We believe that the electrocatalysis between nucleic acid-tethered MB and Fe(CN)6 3– is highly promising for electrochemical nucleic acid-based sensors to achieve better specificity and sensitivity.

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Achievements and challenges in resolving molecular aspects of DNA-protein interactions

Brand

2023

Current Opinion in Electrochemistry

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Direct Detection and Discrimination of Nucleotide Polymorphisms Using Anthraquinone Labeled DNA Probes

Cited by 6 publications

References 72 publications

Recent progress of conductive 3D-printed electrodes based upon polymers/carbon nanomaterials using a fused deposition modelling (FDM) method as emerging electrochemical sensing devices

Recent progress of conductive 3D-printed electrodes based upon polymers/carbon nanomaterials using a fused deposition modelling (FDM) method as emerging electrochemical sensing devices

Use of Electrocatalysis for Differentiating DNA Polymorphisms and Enhancing the Sensitivity of Electrochemical Nucleic Acid-Based Sensors with Covalent Redox Tags—Part II

Achievements and challenges in resolving molecular aspects of DNA-protein interactions

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