Direct demonstration of spatial water distribution in the sponge Suberites domuncula by in vivo NMR imaging

Bringmann, Gerhard; Wolf, Kristina K.; Lanz, Titus; Haase, Axel; Hiort, Jan; Proksch, Peter; Müller, Werner

doi:10.3354/meps189307

Cited by 4 publications

(2 citation statements)

References 7 publications

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“…NMR imaging studies in environmental microbiology have been conducted on microbial mats (Wieland et al 2001), biofilms (e.g. Manz et al 2003, Majors et al 2005) and sponges (Bringmann et al 1999). Application of NMR microscopy for the analysis of single cells and aggregates of microorganisms is a fascinating new method that has just been introduced to microbiology (McLean et al 2008).…”

Section: Emerging Techniques For Functional Imaging In Microbial Ecologymentioning

confidence: 99%

Functional and structural imaging of phototrophic microbial communities and symbioses

Kühl¹,

Polerecký²

2008

Aquat. Microb. Ecol.

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In recent years, new imaging methods for mapping microhabitats, solute concentrations, microbial activity and photopigment content have been developed and increasingly applied in different areas of aquatic microbial ecology and biogeochemistry. Such techniques exhibit a spatial resolution similar to that of microsensors, but allow for instantaneous mapping of the 2-dimensional distribution and dynamics of solute and pigment concentrations over areas ranging from µm 2 to several cm 2 . Methods have been developed for imaging O 2 and CO 2 concentrations as well as pH with optical sensor foils (i.e. planar optodes), and for combined imaging of photopigments and photosynthetic activity via variable chlorophyll fluorescence. More recently, hyperspectral imaging has been employed to identify pigment content in individual cells and to visualize the spatial organization of functional groups in photosynthetic microbial communities. We give an overview of these emerging methods with a range of illustrative examples to demonstrate how they can provide useful information in functional and structural studies of a variety of aquatic microbial communities, such as sediments, biofilms, coral tissues or photosynthetic mats.

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Section: Emerging Techniques For Functional Imaging In Microbial Ecologymentioning

confidence: 99%

Functional and structural imaging of phototrophic microbial communities and symbioses

Kühl¹,

Polerecký²

2008

Aquat. Microb. Ecol.

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“…Therefore, a noninvasive method had to be applied to analyze and quantitate the amount of free mobile water. In an earlier approach to determine the local distribution/amount of water in an intact sponge specimen, the non-invasive technique of nuclear magnetic resonance (NMR) imaging was successfully applied (Bringmann et al, 1999). Now a related NMR technique which is even more sensitive, was chosen, which discriminates structures in the submillimeter range, down to 5 m isotropic voxel resolution for living biological systems, was applied.…”

Section: Discussionmentioning

confidence: 99%

Magnetic resonance imaging of the siliceous skeleton of the demosponge Lubomirskia baicalensis

Müller

Kaluzhnaya

Беликов

et al. 2006

Journal of Structural Biology

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The skeletal elements (spicules) of the demosponge Lubomirskia baicalensis were analyzed; they are composed of amorphous, noncrystalline silica, and contain in a central axial canal the axial Wlament which consists of the enzyme silicatein. The axial Wlament, that orients the spicule in its longitudinal axis exists also in the center of the spines which decorate the spicule. During growth of the sponge, new serially arranged modules which are formed from longitudinally arranged spicule bundles are added at the tip of the branches. X-ray analysis revealed that these serial modules are separated from each other by septate zones (annuli). We describe that the longitudinal bundles of spicules of a new module originate from the apex of the earlier module from where they protrude. A cross section through the oscular/apical-basal axis shows that the bundle rays are organized in a concentric and radiate pattern. High resolution magnetic resonance microimaging studies showed that the silica spheres of the spicules in the cone region contain high amounts of 'mobile' water. We conclude that the radiate accretive growth pattern of sponges is initiated in the apical region (cones) by newly growing spicules which are characterized by high amounts of 'mobile' water; subsequently spicule bundles are formed laterally around the cones. 

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Full Absolute Stereostructures of Natural Products Directly from Crude Extracts: The HPLC-MS/MS-NMR-CD ‘Triad’

Bringmann

Lang

2003

Sponges (Porifera)

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Direct demonstration of spatial water distribution in the sponge Suberites domuncula by in vivo NMR imaging

Cited by 4 publications

References 7 publications

Functional and structural imaging of phototrophic microbial communities and symbioses

Functional and structural imaging of phototrophic microbial communities and symbioses

Magnetic resonance imaging of the siliceous skeleton of the demosponge Lubomirskia baicalensis

Full Absolute Stereostructures of Natural Products Directly from Crude Extracts: The HPLC-MS/MS-NMR-CD ‘Triad’

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