Direct current electrogenerated chemiluminescent microdetermination of peroxydisulfate in aqueous solution

Yamashita, Kazuo; Yamazaki-Nishida, Suzuko; Harima, Yutaka; Segawa, Akihiro.

doi:10.1021/ac00009a006

Cited by 55 publications

(27 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Electrochemiluminescence of the Ru(bpy) 2 , correlation coef®cient: 0.999, n 6). This result is comparable to that of the Ru(bpz) 3 2 electrochemiluminescent method at a stationary working electrode [18,19]. At S 2 O 2À 8 concentrations higher than 1 mmol L À1 , the intensity drops off sharply and is completely suppressed (`0.005 % of the maximum) at 100 mmol L À1 (Fig.…”

Section: àsupporting

confidence: 75%

“…Several methods have been developed for the determination of S 2 O 2À 8 , for example, a reductimetric method, a potentiometric method, a¯ame analytical method, and a Ru(bpz) 2 3 electrochemiluminescent method [14±19]. Among these methods, the Ru(bpz) 2 3 electrochemiluminescent method is one of the most suitable methods for the microdetermination of S 2 O 2À 8 in aqueous solution [18,19]; however, Ru(bpz) 2 3 is relatively expensive and the Ru(bpz) 2 3 electrochemiluminescent method also suffers from electrode fouling [18,19]. It has been reported that ECL of the Ru(bpy) 2 3 aS 2 O 2À 8 system could be obtained in partially aqueous solution and ECL reactions often happened near the surface of the electrode [6].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrochemiluminescence of the Ru(bpy)32+/S2O82- System in Purely Aqueous Solution at Carbon Paste Electrode

Dong

2000

Electroanalysis

View full text Add to dashboard Cite

show abstract

Section: àsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Electrochemiluminescence of the Ru(bpy)32+/S2O82- System in Purely Aqueous Solution at Carbon Paste Electrode

Dong

2000

Electroanalysis

View full text Add to dashboard Cite

show abstract

“…Nevertheless, at pH above 7.0, the ECL enhancement decreased, because more basic solutions would make H 2 O 2 unstable 24 and lead to the consumption of SO 4 •− by the scavenging reaction with OH − . 34 Therefore, the number of HO…”

Section: Resultsmentioning

confidence: 99%

A Dual-Coreactants Electrochemiluminescent Immunosensor for Procalcitonin Detection Based on CdS-MoS₂Nanocomposites

Chen

Xia

Tian

et al. 2018

J. Electrochem. Soc.

View full text Add to dashboard Cite

A novel dual-coreactants electrochemiluminescent (ECL) immunosensor based on CdS-MoS 2 nanocomposites was designed for detection of procalcitonin (PCT). The CdS-MoS 2 nanocomposites were synthesized through a simple one-pot hydrothermal method, and served as highly efficient luminescent material for the ECL immunosensor. In addition, we used H 2 O 2 and K 2 S 2 O 8 as dualcoreactants, generating stronger ECL emission than that of K 2 S 2 O 8 or H 2 O 2 as an individual coreactant, which could improve sensitivity of the immunosensor. Under optimum conditions, the ECL assay for PCT detection was developed with excellent sensitivity of a concentration variation from 0.0001 to 10 ng mL −1 and limit of detection down to 33 fg mL −1 (RSD = 0.10). Additionally, the proposed immunosensor showed high specificity, good reproducibility, and long-term stability.

show abstract

“…In the past 15 years, many analytical applications of ECL using the Ru chelate have been studied for measuring chemical materials such as carbonate compounds, amine compounds and others 2,3 , and for detecting the total DNA. 4,5 In the former measurement system, the ECL signal linearity was 1000 to 10000 times of magnitude in accordance with the analyte concentration.…”

Section: Ecl Emitting Efficiency By Molecular Size Of Eclproducing Mamentioning

confidence: 99%

“…[2][3][4][5] A preliminary ECL immunoassay was reported by Bard in 1984, using a newly synthesized Ru-chelate having N-hydroxysuccinimide residue (Ru-chelate-NHS), which enabled the protein moiety to be labeled 6 (Fig. 1).…”

mentioning

confidence: 99%

Highly Sensitive Electrochemiluminescence Immunoassay Using the Ruthenium Chelate-Labeled Antibody Bound on the Magnetic Micro Beads

1999

View full text Add to dashboard Cite

Since the ECL phenomenon with ruthenium(II) trisbipyridine (Ru(bpy) 3 2+ : Ru-chelate) in an aqueous solution was identified by Rubinstein in 1981 1 , Bard and his coworkers showed many applications of ECL for the measurement of chemical and/or biological substances such as oxalate and total DNA measurement. [2][3][4][5] A preliminary ECL immunoassay was reported by Bard in 1984, using a newly synthesized Ru-chelate having N-hydroxysuccinimide residue (Ru-chelate-NHS), which enabled the protein moiety to be labeled 6 (Fig. 1). Further studies on ECL immunoassays were carried out by Igen International. In 1990, Leland improved the sensitivity of ECL detection by adding tripropylamine (TPA) to the electrolyte solution; this showed a strong reductant activity after electrochemical oxidation.7 This catalytic reaction of oxidized TPA extended the detection limit of the Ru-chelate to 0.2 pmol, which was approximately 10 thousand times more sensitive than that initially reported by Ege in 1984. 8 Meanwhile, the first actual ECL immunoassay was reported by Ikariyama et al. in 1985, using the pyrene labeled human serum albumin (HSA) as the competitive and homogeneous immunoassay method. 9 Their report demonstrated that the intensities of the photon emission were correlated with the amounts of the analyte antigen that did not emit the photon.Generally, an electrochemical reaction occurs within a limited reaction layer, the so-called electrical double layer and/or the electrical diffuse layer at several nm distances from the surface of the working electrode (WE). Consequently, the ECL emission might be interfered with by the increase in molecule size of the labeled HSA, if they bonded with the corresponding antibody molecule having a size of ten and several nm. Thus, the major ECL emission reported by Ikariyama et al. might be caused by non-reacted labeled HAS, because its molecular size is approximately 30% of the labeled HSA binding with the antibody.Based on the above considerations, only an extremely small quantity of the analyte that contacts with the sur- The newer electrochemiluminescence (ECL) immunoassay system was established by using both an antibody coated on paramagnetic micro-beads (Capt-MB) as a carrier of the immunoassay and an antibody labeled with ruthenium(II) trisbipyridine-NHS (Ru-Ab). The ECL excitations were designed to be generated upon the surface of the working electrode which collected the reacted Capt-MB by magnetic force. As a model of the immunoassay, the reaction between alphafetoprotein (AFP) and anti-AFP antibodies was used in accordance with the so-called sandwich method, where the sandwich conformation of AFP-(Ru-Ab) was made on the surface of the Capt-MB. The ECL immunoassay system revealed the following results: a) the ECL signal intensity was obtained in proportion to the AFP concentration of each specimen; b) the dynamic range of this ECL immunoassay system was extended to 10000 times of magnitude in the 2-step assay; and c) the detection sensitivity reached to the level of 5 pg...

show abstract

Direct current electrogenerated chemiluminescent microdetermination of peroxydisulfate in aqueous solution

Cited by 55 publications

References 26 publications

Electrochemiluminescence of the Ru(bpy)32+/S2O82- System in Purely Aqueous Solution at Carbon Paste Electrode

Electrochemiluminescence of the Ru(bpy)32+/S2O82- System in Purely Aqueous Solution at Carbon Paste Electrode

A Dual-Coreactants Electrochemiluminescent Immunosensor for Procalcitonin Detection Based on CdS-MoS₂Nanocomposites

Highly Sensitive Electrochemiluminescence Immunoassay Using the Ruthenium Chelate-Labeled Antibody Bound on the Magnetic Micro Beads

Contact Info

Product

Resources

About

Direct current electrogenerated chemiluminescent microdetermination of peroxydisulfate in aqueous solution

Cited by 55 publications

References 26 publications

Electrochemiluminescence of the Ru(bpy)32+/S2O82- System in Purely Aqueous Solution at Carbon Paste Electrode

Electrochemiluminescence of the Ru(bpy)32+/S2O82- System in Purely Aqueous Solution at Carbon Paste Electrode

A Dual-Coreactants Electrochemiluminescent Immunosensor for Procalcitonin Detection Based on CdS-MoS2Nanocomposites

Highly Sensitive Electrochemiluminescence Immunoassay Using the Ruthenium Chelate-Labeled Antibody Bound on the Magnetic Micro Beads

Contact Info

Product

Resources

About

A Dual-Coreactants Electrochemiluminescent Immunosensor for Procalcitonin Detection Based on CdS-MoS₂Nanocomposites