Direct culture–independent sequence typing of <i>Taylorella equigenitalis</i> obtained from genital swabs and frozen semen samples from South African horses

May, Catherine Edith; Guthrie, Alan John; Schulman, Martin L.

doi:10.1177/1040638719871089

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…Furthermore, the use of swabs in Amies medium with charcoal could explain why it was only possible to carry out characterization in 11 out of the 39 real-time PCR samples considering possible inhibition caused by agar (Gibb and Wong, 1998). Therefore, the procedure carried out in this study could be an option for samples yielding a negative result by culture but a positive result by real-time PCR, but also for clinical samples avoiding a prior culture step, as already carried out by other authors with T. equigenitalis (May et al, 2019).…”

Section: Discussionmentioning

confidence: 74%

First report and molecular characterization of cases of natural Taylorella asinigenitalis infection in three donkey breeds in Spain

Dorrego

Herranz

Camino

et al. 2023

Veterinary Microbiology

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Section: Discussionmentioning

confidence: 74%

First report and molecular characterization of cases of natural Taylorella asinigenitalis infection in three donkey breeds in Spain

Dorrego

Herranz

Camino

et al. 2023

Veterinary Microbiology

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“…1 . If an isolate were required for archiving or bacteriological typing, a horse diagnosed as positive for T. equigenitalis by PCR could be re-sampled using transport medium and culture performed at this stage, although typing by multilocus sequence typing (MLST) has been successfully used on qPCR products obtained directly from swabs without the need for a culture isolate [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

Enhanced detection of <i>Taylorella equigenitalis</i> by qPCR using ‘Dry’ swabs

Mawhinney

BOLLARD²

2023

JES

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Detection of Taylorella equigenitalis (CEMO) in the horse uses genital swabs. These swabs traditionally have been put in Amies charcoal transport medium for detection by culture but are also used for PCR. We determined the suitability of swabs without transport medium (Dry swabs) for CEMO PCR compared to swabs in Amies charcoal transport medium. The experiment was a factorial design using swab type and dilution of organism in culture suspensions, done in two parts. Simulated genital swabs were prepared in the laboratory by dipping in pairs into culture suspensions containing T. equigenitalis with or without other organisms, and then inserting them into a sleeve either with or without transport medium.In study 1, the difference in Ct value for the two swab types was compared. In study 2 genital swab material was then also added to culture suspensions and the swab types again compared. The swabs were tested by a validated quantitative PCR method. The Ct value of the PCR test was used as the measure for comparison, and the effect of variables assessed with linear regression. There was an 7.7% (6.5-8.9) higher mean Ct value of TM versus Dry swabs (P<0.001) overall. The Ct difference was more marked at higher dilutions. Addition of genital swab material had no effect on the Ct value. Dry swabs perform at least as well for PCR as swabs in Amies charcoal transport medium, especially when relatively low numbers of organism are present, and are advantageous for routine sampling when culture is not being used.

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“…The DNA was extracted from the swabs and qPCR conducted as previously described 18. Positive samples were subjected to multilocus sequence typing (MLST) analysis as previously described 21…”

Section: Investigationsmentioning

confidence: 99%

In‐treatment monitoring and use of a novel topical therapy shorten the duration to eliminate Taylorella asinigenitalis in donkey stallions

May

Guthrie

Schulman

2020

Vet Record Case Reports

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Postarrival quarantine testing of a consignment of imported miniature donkeys (Equus asinus) with a duplex Taylorella equigenitalis/asinigenitalis real-time quantitative PCR (qPCR) assay and bacteriological culture identified T asinigenitalis in one donkey stallion from the third set of samples collected at weekly intervals. Following transportation to an approved quarantine station, further testing showed the second companion donkey stallion to be additionally positive for T asinigenitalis. Daily topical treatment included the off-label application of a bovine intramammary antimicrobial preparation to the predilection sites with concurrent in-treatment sampling for qPCR to determine treatment end point. Treatment successfully eliminated colonisation with the organism in both donkey stallions within seven days. This report strongly supported multiple sampling opportunities and the inclusion of a duplex qPCR during postimportation screening. The modified treatment regimen appeared to facilitate the rapid interval to elimination of the organism and the prompt determination of treatment end point.

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Direct culture–independent sequence typing of Taylorella equigenitalis obtained from genital swabs and frozen semen samples from South African horses

Cited by 4 publications

References 21 publications

First report and molecular characterization of cases of natural Taylorella asinigenitalis infection in three donkey breeds in Spain

First report and molecular characterization of cases of natural Taylorella asinigenitalis infection in three donkey breeds in Spain

Enhanced detection of <i>Taylorella equigenitalis</i> by qPCR using ‘Dry’ swabs

In‐treatment monitoring and use of a novel topical therapy shorten the duration to eliminate Taylorella asinigenitalis in donkey stallions

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