Direct contact test for estimating the ecotoxicity of aerosol samples

Kováts, Nóra; Ács, András; Kovács, Anikó; Ferincz, Árpád; Turóczi, B.; Gelencsér, András

doi:10.1016/j.etap.2011.12.021

Cited by 25 publications

(10 citation statements)

References 11 publications

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“…In this case, the toxicity value of Cu 2+ was the highest in 30 minutes, while it was seen that the toxicity value was decreased as the exposure time increased in other samples (3,5 -DCA and aniline). Toxicity was measured with ToxAlert 100 and Flash in the study of Kovats et al [37]. It was found that the luminescent bacterium test (Microtox/ToxAlert) turbidity and color of the sample might cause a significant decrease of light output due to physical effects, creating the potential for false-positives.…”

Section: Comparison Of Acute Toxicity Tests With Each Othermentioning

confidence: 99%

Change of Acute Toxicity of Dyestuff Wastewaters

Sönmez¹,

Sivri²

2019

Pol. J. Environ. Stud.

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Dyes from different sources often behave with mostly toxic properties when they enter the aquatic ecosystem. The main sources of these toxic substances causing problems in the aquatic ecosystem are wastewaters of the textile and dye industries. The aim of this study was to examine the change of acute toxicity values of wastewaters containing dyestuff depending on time and color parameters. In this study, an acute toxicity test was conducted for 30 seconds, which is shorter than the time specified in the original Microtox test protocol. Thus, the results of the original Microtox Basic Test (BT), Microtox Color Correction Procedure recommended by the manufacturer (BT-CC), and the Microtox Basic Test-30 seconds (sBT) were compared, taking into account the effect of time and color parameters. There is a statistically significant difference between two groups: sBT and BT, and sBT and BT-CC (F = 4.919; p<0.01). The results of sBT have been obtained more rapid and more sensitively than other acute toxicity tests. The sBT, which is time-saving and gives correct result might provide some guidance as a pre-screening test and might be used in routine monitoring.

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Section: Comparison Of Acute Toxicity Tests With Each Othermentioning

confidence: 99%

Change of Acute Toxicity of Dyestuff Wastewaters

Sönmez¹,

Sivri²

2019

Pol. J. Environ. Stud.

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“…With respect to the studies assessing ecotoxicological effects of combustion particles, either extracts in water or DMSO were used for testing [25, 66-69, 91, 92] or the filters were directly used (either grounded or as inserts in well plates) for exposing the bacteria [7,23,24,90,93].…”

Section: Overview On Studiesmentioning

confidence: 99%

From the air to the water phase: implication for toxicity testing of combustion-derived particles

Schmidt

Altenburger

Kühnel

2017

Biomass Conv. Bioref.

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The biological effects of airborne particulate matter (PM) in humans stimulated many research activities in recent years. One type of particles contributing to PM are those derived from combustion processes. By using in vitro test systems for assessing the toxic effects of those particles, the transfer into liquid media is necessary. Thus the question arises, how the transfer of airborne particles into liquid suspensions should be realized and how the exposure of test organisms/cells to the particles can be done and monitored with regard to concentration and stability. Further, the characterization of the airborne particles regarding their composition seems to be of importance in order to understand the induced biological effects. Hence, this review gathers and evaluates results from in vitro and ecotoxicity studies, where the introduction of airborne particles into liquids is required. The aim was to learn on the current practice and recommend on the design of toxicity studies in the future. In total, about 80 papers were retrieved by literature search that deal with the assessment of biological effects of combustion-derived particles or their constituents. The papers were evaluated in detail with regard to the methodology applied. From this evaluation and based on expert knowledge regarding nanoparticle testing, recommendations for the assessment of PM applying toxicological assays were developed. To provide guidance, essential criteria are developed that should help in preparation, testing, and during the evaluation phase of toxicological assays. We further advise whole particle testing, complemented by testing of particle extracts and washed particles.

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“…The sampling times were 2 h. In the case of emission samples both the conditions of sampling and the short sampling times ensure that the contribution of ambient PM is practically negligible. Since aerosol samples are collected on filters and ecotoxicity is to be measured in aqueous suspensions, a novel sample preparation protocol was developed (Kováts et al, 2012). 2 mg of resuspended road dust samples were weighted with a microbalance (10 µg sensitivity) into 4 ml pre-weighted vials.…”

Section: Sample Collectionmentioning

confidence: 99%

“…Very recently we have developed a simple method for the direct assessment of the overall ecotoxicity of PM 2.5 / PM 10 (Kováts et al, 2012). This method is based on the kinetic version of the Vibrio fischeri bioluminescence inhibition bioassay (Lappalainen et al, 1999) that has recently been standardized for the assessment of coloured suspensions such as sludge or sediment (ISO 21338:2010: Water quality -Kinetic determination of the inhibitory effects of sediment, other solids and coloured samples on the light emission of Vibrio fischeri /kinetic luminescent bacteria test/).…”

Section: Introductionmentioning

confidence: 99%

Comparative assessment of ecotoxicity of urban aerosol

Turóczi

Hoffer

Tóth³

et al. 2012

Atmos. Chem. Phys.

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Abstract. In addition to its mass concentration, the health effects of urban particulate matter may depend on its particle size distribution and chemical composition. Yet air pollution regulations rely on exclusively bulk PM 10 concentration measurements, without regard to their potentially different health effects under different conditions. Aerosols from various sources are well known to contain a plethora of toxic, carcinogenic, mutagenic or teratogenic constituents such as heavy metals and polycyclic aromatic hydrocarbons. Extensive public health studies established the link between mass concentrations of PM 2.5 / PM 10 and health problems within the population. However, little is known about the relative importance of PM from different sources and the effect of seasonality on the toxicity. Here we present the application of a simple and sensitive method for the direct assessment of the overall ecotoxicity of various PM 2.5 / PM 10 samples collected on filters. The method is based on the Vibrio fischeri bioluminescence inhibition bioassay that has been standardized for solid samples, representing a relevant biological exposure route. Direct emission samples proved to be significantly more ecotoxic than photochemically processed aerosol, thus marked differences were observed between the ecotoxicities of urban PM 10 in summer and winter. These effects of urban PM 10 may be useful supplementary indicators besides the mass concentrations of PM 2.5 / PM 10 in cities.

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Direct contact test for estimating the ecotoxicity of aerosol samples

Cited by 25 publications

References 11 publications

Change of Acute Toxicity of Dyestuff Wastewaters

Change of Acute Toxicity of Dyestuff Wastewaters

From the air to the water phase: implication for toxicity testing of combustion-derived particles

Comparative assessment of ecotoxicity of urban aerosol

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