Direct Comparison of DNA versus mRNA on the Efficiency of Induced Pluripotent Stem Cells Generation from Human Primary Fibroblasts

Carpio, ra Aurora Telpalo; Díaz‐Mitoma, Francisco; Cuevas, Jorge; Aguilar‐Yáñez, José Manuel

doi:10.21767/2573-5365.100013

Cell Mol Med

2016

DOI: 10.21767/2573-5365.100013

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Direct Comparison of DNA versus mRNA on the Efficiency of Induced Pluripotent Stem Cells Generation from Human Primary Fibroblasts

ra Aurora Telpalo Carpio¹,

Francisco Díaz‐Mitoma²,

Jorge Cuevas³

et al.

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Production of endothelial progenitor cells from skin fibroblasts by direct reprogramming for clinical usages

Pham

Dao

et al. 2016

In Vitro Cell.Dev.Biol.-Animal

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Endothelial progenitor cells (EPCs) play an important role in angiogenesis. However, they exist in limited numbers in the human body. This study was aimed to produce EPCs, for autologous transplantation, using direct reprogramming of skin fibroblasts under GMP-compliant conditions. Fibroblasts were collected and cultured from the skin in DMEM/F12 medium supplemented with 5% activated platelet-rich plasma and 1% antibiotic-antimycotic solution. They were then transfected with mRNA ETV2 and incubated in culture medium under hypoxia (5% oxygen) for 14 d. Phenotype analysis of transfected cells confirmed that single-factor ETV2 transfection successfully reprogrammed dermal fibroblasts into functional EPCs. Our results showed that ETV2 mRNA combined with hypoxia can give rise to functional EPCs. The cells exhibited functional phenotypes similar to endothelial cells derived from umbilical cord vein; they expressed CD31 and VEGFR2, and formed capillary-like structures in vitro. Moreover, these EPCs could significantly improve hindlimb ischemia in mouse models. Although the direct conversion efficacy was low (3.12 ± 0.98%), altogether our study demonstrates that functional EPCs can be produced from fibroblasts and can be used in clinical applications.

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Production of endothelial progenitor cells from skin fibroblasts by direct reprogramming for clinical usages

Pham

Dao

et al. 2016

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

show abstract

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Direct Comparison of DNA versus mRNA on the Efficiency of Induced Pluripotent Stem Cells Generation from Human Primary Fibroblasts

Cited by 1 publication

References 40 publications

Production of endothelial progenitor cells from skin fibroblasts by direct reprogramming for clinical usages

Production of endothelial progenitor cells from skin fibroblasts by direct reprogramming for clinical usages

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