Direct comparison of current cell-based and cell-free approaches towards the repair of craniofacial bone defects – A preclinical study

Corre, Pierre; Merceron, Christophe; Longis, J.; Khonsari, R.H.; Pilet, Paul; Thi, Trang; Battaglia, Séverine; Sourice, Sophie; Masson, Martial; Sohier, Jérôme; Espitalier, F.; Guicheux, Jérôme; Weiss, Pierre

doi:10.1016/j.actbio.2015.08.013

Cited by 13 publications

(10 citation statements)

References 86 publications

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“…In general, a higher positive effect on bone formation has been demonstrated when bone affiliated cells were used ( Boerckel et al, 2012 ; Corre et al, 2015 ; Dupont et al, 2010 ; Jäger et al, 2007 ; Liu et al, 2013 ; Montjovent et al, 2008 ; Srouji and Livne, 2005 ; Xu et al, 2010 ; Yasko et al, 1992 ), which is in contradiction with our results. What may be responsible for these differences in the final outcomes on bone formation are the variations in technical and experimental designs.…”

Section: Discussioncontrasting

confidence: 99%

“…In different experimental and in vivo implantation conditions, those cells have demonstrated their capacity to induce bone formation when implanted with BTE scaffolds. In citing a few studies, Serafini et al have highlighted the ability of bone marrow-derived MSCs to form bone marrow and hematopoietic niches when implanted in heterotopic sites ( Serafini et al, 2014 ), whereas other studies have shown an increase in bone formation when implanted in bone sites ( Corre et al, 2015 ; Dupont et al, 2010 ; Jäger et al, 2007 ; Liu et al, 2013 ; Montjovent et al, 2008 ; Srouji and Livne, 2005 ; Xu et al, 2010 ; Yasko et al, 1992 ).…”

Section: Introductionmentioning

confidence: 99%

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Effect of temporal onsets of mechanical loading on bone formation inside a tissue engineering scaffold combined with cell therapy

et al. 2018

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Several approaches to combine bone substitutes with biomolecules, cells or mechanical loading have been explored as an alternative to the limitation and risk-related bone auto- and allo-grafts. In particular, human bone progenitor cells seeded in porous poly(L-lactic acid)/tricalcium phosphate scaffolds have shown promising results. Furthermore, the application of mechanical loading has long been known to be a key player in the regulation of bone architecture and mechanical properties. Several in vivo studies have pointed out the importance of its temporal offset. When an early mechanical loading was applied a few days after scaffold implantation, it was ineffective on bone formation, whereas a delayed mechanical loading of several weeks was beneficial for bone tissue regeneration. No information is reported to date on the effectiveness of applying a mechanical loading in vivo on cell-seeded scaffold with respect to bone formation in a bone site. In our study, we were interested in human bone progenitor cells due to their low immunogenicity, sensitivity to mechanical loading and capacity to differentiate into osteogenic human bone progenitor cells. The latest capacity allowed us to test two different bone cell fates originating from the same cell type. Therefore, the general aim of this study was to assess the outcome on bone formation when human bone progenitor cells or pre-differentiated osteogenic human bone progenitor cells are combined with early and delayed mechanical loading inside bone tissue engineering scaffolds. Scaffolds without cells, named cell-free scaffold, were used as control. Surprisingly, we found that (1) the optimal solution for bone formation is the combination of cell-free scaffolds and delayed mechanical loading and that (2) the timing of the mechanical application is crucial and dependent on the cell type inside the implanted scaffolds.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of temporal onsets of mechanical loading on bone formation inside a tissue engineering scaffold combined with cell therapy

et al. 2018

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“…www.nature.com/scientificreports www.nature.com/scientificreports/ Tissue engineering is potentially a promising alternative to autologous vascularized bone grafting for the reconstruction of large skeletal defects [26][27][28][29] . Numerous pre-clinical studies have demonstrated that the combination of mesenchymal stem cells (MSCs) with BCP biomaterials can induce bone formation and facilitate the healing of critical size defects [30][31][32][33] . Some clinical trials have demonstrated the feasibility of amplifying autologous MSCs in culture from a bone marrow sample and associating them with BCP biomaterial to regenerate unconsolidated fractures or small defects 8,34 .…”

Section: Discussionmentioning

confidence: 99%

Regeneration of segmental defects in metatarsus of sheep with vascularized and customized 3D-printed calcium phosphate scaffolds

Vidal

Kampleitner

Krissian

et al. 2020

Sci Rep

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Although autografts are considered to be the gold standard treatment for reconstruction of large bone defects resulting from trauma or diseases, donor site morbidity and limited availability restrict their use. Successful bone repair also depends on sufficient vascularization and to address this challenge, novel strategies focus on the development of vascularized biomaterial scaffolds. This pilot study aimed to investigate the feasibility of regenerating large bone defects in sheep using 3D-printed customized calcium phosphate scaffolds with or without surgical vascularization. Pre-operative computed tomography scans were performed to visualize the metatarsus and vasculature and to fabricate customized scaffolds and surgical guides by 3D printing. Critical-sized segmental defects created in the mid-diaphyseal region of the metatarsus were either left empty or treated with the 3D scaffold alone or in combination with an axial vascular pedicle. Bone regeneration was evaluated 1, 2 and 3 months postimplantation. After 3 months, the untreated defect remained non-bridged while the 3D scaffold guided bone regeneration. the presence of the vascular pedicle further enhanced bone formation. Histology confirmed bone growth inside the porous 3D scaffolds with or without vascular pedicle inclusion. Taken together, this pilot study demonstrated the feasibility of precised pre-surgical planning and reconstruction of large bone defects with 3D-printed personalized scaffolds. Bone is a dynamic tissue that possesses the intrinsic capacity to heal within 6-8 weeks after immobilization of a fracture. However, there are some conditions in which bone regeneration is delayed, compromised or beyond the physiological healing potential 1,2. Notably, the successful repair of large bone defects caused by trauma, tumor resection or disease remains a clinical challenge for orthopedic and plastic surgeons and often requires additional treatments. Autologous bone grafting is still considered the gold standard treatment due to its osteoconductive, osteoinductive and osteogenic properties. This procedure necessitates harvesting the patient's own bone and subsequently transplanting it to the defect site. Bone can be taken from several areas e.g., iliac crest or fibula, depending on the severity and amount needed for reconstruction of the defect. Nevertheless, the amount of

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“…The substantial bone healing in these critical-size defects implanted with BP is of particular interest as bone regeneration in this model is a challenge (Corre et al, 2015;Hivernaud et al, 2019;do Lago et al, 2020;Paré et al, 2020) and widely clinically used bone substitutes need to be mixed with bone marrow aspirate or growth factors to heal such defects. The bone regeneration observed with the BP is a promising result that is reason for further clinical investigation of its regenerative properties in critical and subcritical defects compared to the gold standard of autologous bone grafting.…”

Section: Discussionmentioning

confidence: 99%

“…The defects were left unfilled for the controls or they were filled with BG or BP. The BG were obtained from additional animals euthanized in a CO 2 closed chamber as previously described (Corre et al, 2015). Briefly, the humeri, femurs, and tibiae were harvested, cut lengthwise, and the trabecular bone was collected by scraping (Figure 1A) and then immediately implanted.…”

Section: Calvarial Critical-size Defect Surgical Proceduresmentioning

confidence: 99%

An Extrudable Partially Demineralized Allogeneic Bone Paste Exhibits a Similar Bone Healing Capacity as the “Gold Standard” Bone Graft

Tournier

Guicheux

Paré

et al. 2021

Front. Bioeng. Biotechnol.

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Autologous bone grafts (BGs) remain the reference grafting technique in various clinical contexts of bone grafting procedures despite their numerous peri- and post-operative limitations. The use of allogeneic bone is a viable option for overcoming these limitations, as it is reliable and it has been widely utilized in various forms for decades. However, the lack of versatility of conventional allogeneic BGs (e.g., blocks, powders) limits their potential for use with irregular or hard-to-reach bone defects. In this context, a ready- and easy-to-use partially demineralized allogeneic BG in a paste form has been developed, with the aim of facilitating such bone grafting procedures. The regenerative properties of this bone paste (BP) was assessed and compared to that of a syngeneic BG in a pre-clinical model of intramembranous bone healing in critical size defects in rat calvaria. The microcomputed tridimensional quantifications and the histological observations at 7 weeks after the implantation revealed that the in vivo bone regeneration of critical-size defects (CSDs) filled with the BP was similar to syngeneic bone grafts (BGs). Thus, this ready-to-use, injectable, and moldable partially demineralized allogeneic BP, displaying equivalent bone healing capacity than the “gold standard,” may be of particular clinical relevance in the context of oral and maxillofacial bone reconstructions.

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Direct comparison of current cell-based and cell-free approaches towards the repair of craniofacial bone defects – A preclinical study

Cited by 13 publications

References 86 publications

Effect of temporal onsets of mechanical loading on bone formation inside a tissue engineering scaffold combined with cell therapy

Effect of temporal onsets of mechanical loading on bone formation inside a tissue engineering scaffold combined with cell therapy

Regeneration of segmental defects in metatarsus of sheep with vascularized and customized 3D-printed calcium phosphate scaffolds

An Extrudable Partially Demineralized Allogeneic Bone Paste Exhibits a Similar Bone Healing Capacity as the “Gold Standard” Bone Graft

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