Direct clean-up and analysis of ribonucleosides in physiological fluids

Schlimme, E.; Boos, Karl-Siegfried; Hagemeier, E.; Kemper, K.; Meyer, Ulrich; Hobler, H.; Schnelle, T.; Weise, Manfred

doi:10.1016/s0378-4347(00)80731-8

Cited by 23 publications

(5 citation statements)

References 14 publications

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“…4). Levels of urinary nucleotides, including adenine63, adenosine6364, 3-methylcytidine64, uridine6566, thymine67, Pseu6568, m7Gua69, m1A656668, t6A66, m22G6566, and urate70, are reported to be significantly elevated in patients with various cancers. Increased urate can also be a risk factor for hypertension and CVD in patients71.…”

Section: Discussionmentioning

confidence: 99%

Urinary Biomarkers of Whole Grain Wheat Intake Identified by Non-targeted and Targeted Metabolomics Approaches

Zhu

Wang

et al. 2016

Sci Rep

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Mounting evidence suggests that whole grain (WG) intake plays an important role in chronic disease prevention. However, numerous human studies have failed to produce clear-cut conclusions on this topic. Here, a combination of non-targeted and targeted metabolomics approaches, together with kinetic studies, was used to investigate biomarkers of WG wheat intake and further explore the diet-disease associations. Via these integrated approaches, forty-one compounds were identified as the most discriminating endogenous metabolites after WG versus refined grain (RG) wheat bread consumption. The corresponding biological assessment of these endogenous changes suggests that, in contrast to RG consumption, WG wheat consumption may facilitate antioxidant defense systems and moderate the risk factors of cancer, cardiovascular diseases, and other chronic diseases. A panel of urinary markers consisting of seven alkylresorcinol metabolites and five benzoxazinoid derivatives as specific biomarkers, as well as five phenolic acid derivatives, was also established to cover multiple time points and longer time periods for correctly and objectively monitoring WG wheat intake. Through these findings, we have established a comprehensive biomarker pool to better assess WG wheat consumption, and to monitor the endogenous changes that are linked to health effects of WG wheat consumption.

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Section: Discussionmentioning

confidence: 99%

Urinary Biomarkers of Whole Grain Wheat Intake Identified by Non-targeted and Targeted Metabolomics Approaches

Zhu

Wang

et al. 2016

Sci Rep

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“…According to [27,28], there is high probability that most nucleosides degrade in solutions at pH above 5.0. Thus, pH adjustment of urine samples is essential to limit the degradation process.…”

Section: Stability Studiesmentioning

confidence: 99%

Development and validation of urinary nucleosides and creatinine assay by capillary electrophoresis with solid phase extraction

Szymańska

Markuszewski

Bodzioch

et al. 2007

Journal of Pharmaceutical and Biomedical Analysis

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“…A number of past studies have indicated that modified nucleosides excreted in the urine have the potential to act as cancer biomarkers with increased levels arising from disease onset and progression 1–4. Modified nucleosides differ from ‘normal’ nucleosides usually by a minor modification, with the most common alteration being methylation.…”

mentioning

confidence: 99%

Analysis of urinary nucleosides. V. Identification of urinary pyrimidine nucleosides by liquid chromatography/electrospray mass spectrometry

Dudley

Lemière

Dongen

et al. 2005

Rapid Comm Mass Spectrometry

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Modified urinary nucleosides are potentially invaluable in cancer diagnosis, as they reflect altered RNA turnovers. High-performance liquid chromatography (HPLC) was combined with full-scan mass spectrometry, tandem mass spectrometry, MS(n) analysis and accurate mass measurements in order to identify pyrimidine nucleosides purified from urine. Potential nucleosides were assessed by their evident UV absorbance in the HPLC chromatogram and then further examined by the various mass spectrometric techniques. In this manner numerous pyrimidine nucleosides were identified in the urine samples from cancer patients including pseudouridine, cytidine, two methylcytidines and an acetylcytidine. Furthermore, a number of novel modified pyrimidine nucleosides were tentatively identified via critical interpretation of the combined mass spectrometric data.

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Direct clean-up and analysis of ribonucleosides in physiological fluids

Cited by 23 publications

References 14 publications

Urinary Biomarkers of Whole Grain Wheat Intake Identified by Non-targeted and Targeted Metabolomics Approaches

Urinary Biomarkers of Whole Grain Wheat Intake Identified by Non-targeted and Targeted Metabolomics Approaches

Development and validation of urinary nucleosides and creatinine assay by capillary electrophoresis with solid phase extraction

Analysis of urinary nucleosides. V. Identification of urinary pyrimidine nucleosides by liquid chromatography/electrospray mass spectrometry

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