Direct capillary electrophoretic detection of carbohydrate-deficient transferrin in neat serum

Trout, Amy L.; Prasad, Rajani; Coffin, David; DiMartini, Andrea; Lane, Tonya; Blessum, Cynthia; Khatter, Neeta; Landers, James P.

doi:10.1002/1522-2683(20000701)21:12<2376::aid-elps2376>3.3.co;2-g

Cited by 8 publications

(12 citation statements)

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“…This patient reported drinking 2 to 3 (12-ounce) beers the week prior to coming to clinic and was not excluded from the study. One other patient was excluded after serendipitously discovering she had a genetic variant of CDT (Trout, 2000), a codominant transferrin deficient allelic (polypeptide) variant that is one of the few definitely known causes of false positive CDT. Though our sample was not screened for such variants, in the Caucasian population these variants account for less than 1% of false-positive values (Stibler, 1987).…”

Section: Methodsmentioning

confidence: 99%

Carbohydrate Deficient Transferrin in Abstaining Patients With End-Stage Liver Disease

DiMartini

Day

Lane

et al. 2001

Alcoholism: Clinical and Experimental Research

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Background-Carbohydrate deficient transferrin (CDT), a biochemical marker of chronic alcohol consumption, is used by researchers and clinicians alike in a variety of populations. Levels of CDT may be affected by certain types of medical illnesses and conditions. Thus the interpretation of CDT results may need to be carefully examined in these populations. Because CDT is synthesized, glycosylated, and secreted by the liver, the use of CDT values in patients with liver disease has been an area of focused interest.

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Section: Methodsmentioning

confidence: 99%

Carbohydrate Deficient Transferrin in Abstaining Patients With End-Stage Liver Disease

DiMartini

Day

Lane

et al. 2001

Alcoholism: Clinical and Experimental Research

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“…In the search for a suitable clinical diagnostic test for alcohol abuse, several analytical procedures, such as IEF (8 ), IEF in combination with immunofixation (9 ), zone immunoelectrophoresis (10 ), Western blotting (11 ), anion-exchange chromatography (7 ), and chromatofocusing (12 ), have been used. The problems encountered in CDT analyses using capillary zone electrophoresis (CZE) (8,13,14 ) were later resolved by an improved CZE method requiring only iron saturation as sample pretreatment (15)(16)(17) and the use of dynamically coated capillaries (18 ). The CEofix TM -CDT assay (Analis) is the first commercial buffer system combining the advantages of dynamic coating with simplicity of sample pretreatment and analysis (19 -22 ).…”

confidence: 99%

Diagnosis of Congenital Disorders of Glycosylation by Capillary Zone Electrophoresis of Serum Transferrin

Carchon

Chevigné²,

Falmagne³

et al. 2004

Clinical Chemistry

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Background: Congenital disorders of glycosylation (CDG) are usually diagnosed by isoelectric focusing (IEF) of serum transferrin (Tf). The aim of this study was to evaluate capillary zone electrophoresis (CZE) as a diagnostic alternative to IEF. Methods: We performed 792 CZE analyses of Tf, using the CEofix TM -CDT (carbohydrate-deficient transferrin) assay. Peak identification was based on relative migration times (RMTs) to reduce migration variability. Results: Tf profiles comprised three main groups (A-C).Groups A and B were characterized by one or two dominant tetrasialo-Tf peaks, whereas group C showed a widely variable Tf isoform composition. Group A was composed of four subgroups: a major group with a typical Tf profile (considered as reference group), two minor groups with decreased or moderately increased trisialo-Tf isoform, and a group showing the presence of unknown compounds with RMTs similar to mono-and disialo-Tf. However, these compounds were absent on IEF. Group C contained all profiles from patients with confirmed as well as putative CDG. From the reference group, 99% confidence intervals were calculated for the RMTs of the Tf isoforms, and percentiles representing the Tf isoform distributions were defined. Conclusions: All patients with abnormal IEF results and confirmed CDG were identified by CZE; thus, this method can be used as a diagnostic alternative to IEF in a manner suitable for automation. Because whole serum is analyzed, it should be kept in mind that CZE profiles can show substances other than Tf.

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“…Trout et al. and Beisler et al. used fluorocarbon‐coated capillaries to achieve good resolution of the Tf isoforms.…”

Section: Ce For Analysis Of Tf Isoformsmentioning

confidence: 99%

“…Neuraminidase cleaves the sialic acid residues located at the end of the two branched carbohydrate structures of Tf and thus influences the electrophoretic mobility and pI of the Tf molecule. Desialylation of serum Tf with neuraminidase was employed to study the progress of this process , to prepare standards of asialo‐Tf , to characterize the progress of desialylation of a human Tf standard by CIEF , and to simplify the identification of genetic variants of Tf . The data presented in Fig.…”

Section: Applicationsmentioning

confidence: 99%

Monitoring of transferrin isoforms in biological samples by capillary electrophoresis

Caslavska

Thormann

2017

J of Separation Science

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Work dealing with the monitoring of transferrin isoforms in human serum and other body fluids by capillary electrophoresis is reviewed. It comprises capillary zone electrophoresis and capillary isoelectric focusing efforts that led to the exploration and use of assays for the determination of carbohydrate‐deficient transferrin as a marker for excessive alcohol intake, genetic variants of transferrin, congenital disorders of glycosylation and β‐2‐transferrin, which is a marker for cerebrospinal fluid leakage. This paper provides insight into the development, specifications, strengths, weaknesses, and routine use of the currently known capillary electrophoresis based assays suitable to detect transferrin isoforms in body fluids. The achievements reached so far indicate that capillary zone electrophoresis is an attractive technology to monitor the molecular forms of transferrin in biological specimens as the assays do not require an elaborate sample pretreatment and thus can be fully automated for high‐throughput analyses on multicapillary instruments. Assays based on capillary isoelectric focusing are less attractive. They require immunoextraction of transferrin from the biological matrix and mobilization after focusing if instrumentation with a whole‐column imaging detector is not available. Interactions of the carrier ampholytes with the iron of transferrin may prevent iron saturation and thus provide more complicated isoform patterns.

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Direct capillary electrophoretic detection of carbohydrate-deficient transferrin in neat serum

Cited by 8 publications

References 0 publications

Carbohydrate Deficient Transferrin in Abstaining Patients With End-Stage Liver Disease

Carbohydrate Deficient Transferrin in Abstaining Patients With End-Stage Liver Disease

Diagnosis of Congenital Disorders of Glycosylation by Capillary Zone Electrophoresis of Serum Transferrin

Monitoring of transferrin isoforms in biological samples by capillary electrophoresis

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