Direct bioethanol production from cellulose by the combination of cellulase-displaying yeast and ionic liquid pretreatment

Nakashima, Kazunori; Yamaguchi, Koji; Taniguchi, Nobuaki; Arai, Shogo; Yamada, R.; Katahira, Satoshi; Ishida, Nobuhiro; Takahashi, Hitomi; Ogino, Chiaki; Kondo, Akihiko

doi:10.1039/c1gc15688h

Cited by 66 publications

(43 citation statements)

References 33 publications

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“…The major focus was on IL tolerance in aqueous systems, however, to minimize water usage in industrial conversion processes, IL tolerant microorganisms that thrive in high solid, thermophilic environments must be considered. Enrichment processes have been used identify microorganisms from green waste compost that can deconstruct switchgrass in the presence of ILs, and these ILtolerant microorganisms can potentially be applied in lignocellulosic bioconversion processes (Reddy et al 2012;Simmons et al 2014 (Nakashima et al 2011). The maximum non-toxic concentrations (MNTC), the concentration at which it is possible to observe microbial growth and viability, was reported for various microorganisms in the presence of ILs (Santos et al 2014).…”

Section: Discovering and Screening For Il Tolerant Microorganisms Relmentioning

confidence: 99%

“…Microorganisms displaying IL tolerance have been screened using direct plating, growth in liquid culture and phenotypic microarrays (Deive et al 2011;Khudyakov et al 2012;Liu et al 2015;Nakashima et al 2011;Petkovic et al 2009;Petkovic et al 2010;Santos et al 2014;Simmons et al 2014;Singer et al 2011;Sitepu et al 2014).…”

Section: Discovering and Screening For Il Tolerant Microorganisms Relmentioning

confidence: 99%

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Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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Chemical and physical pretreatment of biomass is a critical step in the conversion of lignocellulose to biofuels and bioproducts. Ionic liquid (IL) pretreatment has attracted significant attention due to the unique ability of certain ILs to solubilize some or all components of the plant cell wall. However, these ILs not only inhibit enzyme activities, but also the growth and productivity of microorganisms used in downstream hydrolysis and fermentation processes.While pretreated biomass can be washed to remove residual IL and reduce inhibition, extensive washing is costly and not feasible in large-scale processes. IL tolerant microorganisms and microbial communities have been discovered from environmental samples and studies begun to elucidate mechanisms of IL tolerance. The discovery of IL tolerance in environmental microbial communities and individual microbes has lead to the proposal of molecular mechanisms of resistance. In this article, we review recent progress on discovering IL tolerant microorganisms, identifying metabolic pathways and mechanisms of tolerance, and engineering microorganisms for IL tolerance. Research in these areas will yield new approaches to overcome inhibition in lignocellulosic biomass bioconversion processes and increase opportunities for the use of ILs in biomass pretreatment.

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Section: Discovering and Screening For Il Tolerant Microorganisms Relmentioning

confidence: 99%

Section: Discovering and Screening For Il Tolerant Microorganisms Relmentioning

confidence: 99%

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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“…Lignocellulosic biomass has received much attention as a potential feedstock for biofuel production (Lynd et al, 2008). However, lignocellulose is a highly ordered and rigid polymer, and the crystalline structure limits biodegradation by cellulolytic enzymes (Nakashima et al, 2011). To ensure the enzymatic hydrolysis process practical, lignocellulose is typically pretreated by using various procedures (Ouellet et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effects of selected ionic liquids on lipid production by the oleaginous yeast Rhodosporidium toruloides

Huang

Wang

Gong

et al. 2013

Bioresource Technology

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“…Cellulosomes can be applied to various fields including biorefineries, bioadsorption, bioremediation, production of useful chemicals and as biosensors. Cellulosomes can play a major role in the biorefineries for the depolymerization of complex biomass (Nakashima et al 2011). Goyal et al (2011) constructed a minicellulosome by developing a yeast consortium for display of scaffolding protein and secretion of dockerin-fused enzymes for efficient bioethanol production.…”

Section: Applications Of Cellulosomesmentioning

confidence: 99%

Bioprospecting thermostable cellulosomes for efficient biofuel production from lignocellulosic biomass

Arora

Behera

Sharma

et al. 2015

Bioresour. Bioprocess.

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The adverse climatic conditions due to continuous use of fossil-derived fuels are the driving factors for the development of renewable sources of energy. Current biofuel research focuses mainly on lignocellulosic biomass (LCB) such as agricultural, industrial and municipal solid wastes due to their abundance and renewability. Although many mesophilic cellulolytic microorganisms have been reported, efficient and economical bioconversion to simple sugars is still a challenge. Thermostable cellulolytic enzymes play an indispensible role in degradation of the complex polymeric structure of LCB into fermentable sugar stream due to their higher flexibility with respect to process configurations and better specific activity than the mesophilic enzymes. In some anaerobic thermophilic/thermotolerant microorganisms, few cellulases are organized as unique multifunctional enzyme complex, called the cellulosome. The use of cellulosomal multienzyme complexes for saccharification seems to be a promising and cost-effective alternative for complete breakdown of cellulosic biomass. This paper aims to explore and review the important findings in cellulosomics and forward the path for new cutting-edge opportunities in the success of biorefineries. Herein, we summarize the protein structure, regulatory mechanisms and their expression in the host cells. Furthermore, we discuss the recent advances in specific strategies used to design new multifunctional cellulosomal enzymes, which can function as lignocellulosic biocatalysts and evaluate the roadblocks in the yield and stability of such designer thermozymes with overall progress in lignocellulose-based biorefinery.

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Direct bioethanol production from cellulose by the combination of cellulase-displaying yeast and ionic liquid pretreatment

Cited by 66 publications

References 33 publications

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Effects of selected ionic liquids on lipid production by the oleaginous yeast Rhodosporidium toruloides

Bioprospecting thermostable cellulosomes for efficient biofuel production from lignocellulosic biomass

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