Direct bioautography hyphenated to direct analysis in real time mass spectrometry: Chromatographic separation, bioassay and mass spectra, all in the same sample run

Häbe, Tim T.; Jamshidi-Aidji, Maryam; Macho, Jennifer; Morlock, Gertrud E.

doi:10.1016/j.chroma.2018.07.002

Cited by 25 publications

(5 citation statements)

References 28 publications

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“…We have not determined the identity(ies) of the estrogenic substance(s) in the migrates. Although not the purpose of this study, we could further investigate toxicants detected with P-YES by extracting them for chemical analysis such as nontarget high-resolution mass spectrometry [41]. Then, retention factors of standards (if available) from P-YES with chromatography can be used for confirmation.…”

Section: P-yes Reveals Estrogenicity Of Food Contact Materials Where mentioning

confidence: 99%

Estrogenic activity of food contact materials—evaluation of 20 chemicals using a yeast estrogen screen on HPTLC or 96-well plates

et al. 2020

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Food contact materials (FCM) may contain complex mixtures of estrogenic chemicals. A yeast estrogen screen performed on high performance thin-layer chromatography plates (planar-YES, P-YES) is promising for analysis of such mixtures, as it could allow for better elucidation of effects compared with established methods in microtiter plates. However, the P-YES has not been directly compared with established methods. We compared the performance of a microtiter plate YES (lyticase-YES, L-YES) to P-YES on silica gel HPTLC plates using 17β-estradiol (E2), 20 chemicals representative of migrants from plastic FCM, and three migrates of coated metal food cans. Effective doses (ED10, ED50) and estradiol equivalencies were calculated for each chemical. Thirteen chemicals had calculable EDs in the L-YES or P-YES, with average EDs 13-fold (range 0.63–36) more potent in P-YES than in the L-YES. Normalized to E2, the median estrogenicity was within 1.5-fold (0.43–8.8) between the assays. Therefore, P-YES was as or more sensitive than L-YES but potencies relative to E2 were comparable between assays. With chromatography, the P-YES detected estrogenicity in coated metal cans, effects that were unmeasurable in L-YES. With the sample preparation methods used in this study, both YES assays are sufficiently sensitive to detect bisphenol A below the specific migration limit for plastic packaging (0.05 mg/kg food). This study demonstrates that P-YES outperforms L-YES because it is more sensitive, provides comparable estradiol equivalents, and circumvents confounding mixture effects. The P-YES will be useful for routine monitoring of FCM and toxicant identification in problematic materials.

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Section: P-yes Reveals Estrogenicity Of Food Contact Materials Where mentioning

confidence: 99%

Estrogenic activity of food contact materials—evaluation of 20 chemicals using a yeast estrogen screen on HPTLC or 96-well plates

et al. 2020

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“…DB-DART-MS was used to investigate bioactive compounds in cosmetics using the Bacillus subtilis and Aliivibrio fischeri bioassays for detection of Gram-positive and Gram-negative antimicrobials, respectively, and the planar yeast estrogen screen for the detection of estrogen-effective compounds [ 45 ]. Two tansy ( Tanacetum vulgare L.) essential oils were investigated against several kinds of microorganisms, using the coupling of HPTLC-DB.…”

Section: Detection Techniquementioning

confidence: 99%

An Evolving Technology That Integrates Classical Methods with Continuous Technological Developments: Thin-Layer Chromatography Bioautography

et al. 2021

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Thin-layer chromatography (TLC) bioautography is an evolving technology that integrates the separation and analysis technology of TLC with biological activity detection technology, which has shown a steep rise in popularity over the past few decades. It connects TLC with convenient, economic and intuitive features and bioautography with high levels of sensitivity and specificity. In this study, we discuss the research progress of TLC bioautography and then establish a definite timeline to introduce it. This review summarizes known TLC bioautography types and practical applications for determining antibacterial, antifungal, antitumor and antioxidant compounds and for inhibiting glucosidase, pancreatic lipase, tyrosinase and cholinesterase activity constitutes. Nowadays, especially during the COVID-19 pandemic, it is important to identify original, natural products with anti-COVID potential compounds from Chinese traditional medicine and natural medicinal plants. We also give an account of detection techniques, including in situ and ex situ techniques; even in situ ion sources represent a major reform. Considering the current technical innovations, we propose that the technology will make more progress in TLC plates with higher separation and detection technology with a more portable and extensive scope of application. We believe this technology will be diffusely applied in medicine, biology, agriculture, animal husbandry, garden forestry, environmental management and other fields in the future.

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“…This has been addressed to manufacturers since 2005 because in a retrospective view, all chromatographic techniques were boosted and did highly benefit upon hyphenation with MS [ 46 ]. Fully automated elution head-based and desorption-based devices were built and successfully demonstrated in 2007 [ 47 ] and 2018 [ 48 ], respectively. Latest innovation was a cost-effective, open-source add-on kit for automating zone elution [ 49 ] which was designed to fill the longstanding scientific gap and advance HPTLC-MS.…”

Section: Introductionmentioning

confidence: 99%

HI-HPTLC-UV/Vis/FLD-HESI-HRMS and bioprofiling of steviol glycosides, steviol, and isosteviol in Stevia leaves and foods

Morlock

Heil

2020

Anal Bioanal Chem

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Food products and botanicals on the global market need to be investigated in a more comprehensive way to detect effects, falsifications or adulterations. This is especially true for such ones containing Stevia leaves, Stevia extracts, or steviol glycosides. A multi-imaging profiling was developed exploiting hydrophilic interaction liquid chromatography (HILIC). A minimalistic sample preparation, different mixtures of acetonitrile and water/buffer on the silica gel phase as well as derivatization reagents and optional hyphenation with high-resolution mass spectrometry were exploited. The hydrophilic interaction high-performance thin-layer chromatography (HI-HPTLC) development took 10 min for 48 analyses. It was used to screen Stevia leaf extracts and 20 different food products. For the first time, the biological and biochemical profiling of Stevia leaf products by HI-HPTLC-UV/ Vis/FLD-assay pointed to 19 different bioactive compound bands found in the more natural multicomponent Stevia leaf extracts, whereas most of these activities were not existent for the steviol glycosides. The chemically isolated, purified, and EU-regulated steviol glycosides ease risk assessment and food product development. However, multipotent botanicals may have subtle impact on homeostasis via several metabolic pathways, providing benefits for the consumer's health. Analyzed side by side by means of the effect-directed profiling, their individual activity profiles were visualized as image and individual substances of importance were pointed out. Multi-imaging (comprehensive detection) plus non-targeted bioprofiling (focus on known and unknown bioactivity) allows for a fast detection of questionable product changes that occur along the global food chain and are particularly related to food safety.

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Direct bioautography hyphenated to direct analysis in real time mass spectrometry: Chromatographic separation, bioassay and mass spectra, all in the same sample run

Cited by 25 publications

References 28 publications

Estrogenic activity of food contact materials—evaluation of 20 chemicals using a yeast estrogen screen on HPTLC or 96-well plates

Estrogenic activity of food contact materials—evaluation of 20 chemicals using a yeast estrogen screen on HPTLC or 96-well plates

An Evolving Technology That Integrates Classical Methods with Continuous Technological Developments: Thin-Layer Chromatography Bioautography

HI-HPTLC-UV/Vis/FLD-HESI-HRMS and bioprofiling of steviol glycosides, steviol, and isosteviol in Stevia leaves and foods

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