Direct attachment of fibronectin to tresyl chloride‐activated titanium

Hayakawa, Tohru; Yoshinari, Masao; Nemoto, Kimiya

doi:10.1002/jbm.a.10143

Cited by 46 publications

(58 citation statements)

References 22 publications

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“…(34)(35)(36)(37) Biomimetic coating with fibronectin has been tested in previous studies, (20)(21)(22)(23) its adsorption onto solid substrates has already been evaluated in previous studies, though the interactions between this protein and biomaterial surfaces are not fully understood. (30,38) Although many studies reported about the applications of fibronectin to the implant surface can enhance the osseointegration of dental implants to the bone, (39,40) the results of the present study demonstrating the difference between two groups was not statistically significant at both observation periods and this could be due to:…”

Section: Discussioncontrasting

confidence: 52%

Biological Functionalization of Dental Implants with Fibronectin : A Scanning Electron Microscopic Study

Elkarargy¹

2014

IJHS

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Objectives: Early stages of peri-implant bone formation play an essential role in the osseointegration and long-term success of dental implants. Biological implant surface coatings are an emerging technology to enhance the attachment of the implant to the surrounding bone and stimulate bone regeneration. The purpose of this study was to determine the effect of coating the implant surface with fibronectin on osseointegration. Material and methods:The experiment was conducted on a total of twelve New Zealand white mature male rabbits, weight between 2.5-4 kg. Twenty four pure titanium implants were used in this study. Each rabbits received two implants, one implant in each tibia; the implant in the right limb was coated with fibronectin (experimental group), whilst on the contralateral side the implants were placed without coating (control group). Six rabbits were sacrificed for Scanning Electron Microscopic evaluation after 4 and 8 week healing periods. Results:The results of the present study demonstrating the mean gap distance between the bone and implant was greater in the control group compared to fibronection group at both observation periods however, the difference between these two groups was not statistically significant. Conclusion:Thus, it could be suggested that the biological functionalization of dental implants with fibronectin, may influence the integration or biocompatibility and bonding of the implant to the surrounding bone.

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Section: Discussioncontrasting

confidence: 52%

Biological Functionalization of Dental Implants with Fibronectin : A Scanning Electron Microscopic Study

Elkarargy¹

2014

IJHS

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“…1: Uncoated; 2: GRGDSP-coated. 10) . Studies have shown that RGD and related peptides are able to enhance osteoblast adhesion and migration 11,12) .…”

Section: Resultsmentioning

confidence: 99%

“…Synthetic peptides were coated on Ti surfaces using the tresyl chloride activation method described in previous literature 10) . Briefly, Ti disks (JIS, Japan industrial Specification H 4600, 99.9 mass％ Ti; Furuuchi Chemical Corp., Tokyo, Japan) with a diameter of 15 mm or 48 mm were used.…”

Section: Peptide Coating Onto Ti Disksmentioning

confidence: 99%

Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide

et al. 2008

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Enhanced adhesion and migration of osteoblastic cells on titanium (Ti) surface is believed to increase the success rate of implant therapy. A GRGDSP peptide derived from fibronectin was coated on Ti surfaces using a tresyl chloride activation technique, and then MC3T3-E1 osteoblastic cells were cultured on the Ti surfaces. After 15 days, total RNA was isolated from the cells and gene expression level were analyzed by Affymetrix GeneChip system. The expression levels of many genes in MC3T3-E1 cells cultured on GRGDSP-coated Ti surface were altered when compared to uncoated Ti. In particular, the elevated mRNA levels of bone sialoprotein (BSP) and osteocalcin (OC) were successfully confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. In light of the results obtained, GRGDSP-coated Ti presented the potential of evolving into a useful biomaterial for successful implant therapy.

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“…Collagen was immobilized onto 1.0 Laser-Ti implants by the tresyl chloride-activated method 19,20) . This immobilization scheme is shown in Fig.…”

Section: Immobilization Of Collagen With the Tresyl Chloride-activatementioning

confidence: 99%

Bone and Gingival Connective Tissue Responses towards Nanosecond-Pulsed Laser-Treated Titanium Implants

Fukayo

Amemiya

Nakaoka

et al. 2016

J. Hard Tissue Biology.

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:The aim of this study was to evaluate bone and gingival connective tissue responses towards nanosecond-pulsed laser-treated titanium implants. A Nd:YVO 4 nanosecond-pulse laser with a defocus technique was used to modify the surfaces of two types of cylindrical titanium implants. One had a 3.5 mm diameter and 7.0 mm length ( 3.5 Ti) to assess rabbit bone responses; the other a 1.0 mm diameter and 4.5 mm length ( 1.0 Ti) to assess rat gingival connective tissue responses. Laser-treated titanium implants, a 3.5 Laser-Ti and 1.0 Laser-Ti, were obtained by defocus irradiation. Collagen immobilized 1.0Laser-Ti ( 1.0 Coll/Laser-Ti) implants were obtained by a tresyl chloride-activated method. Laser-Ti surfaces had micro-scale roughened oxide layers and parallel arranged grooves. Sa (average roughness) and Sdr (interfacial area ratio) values of the Laser-Ti were significantly higher than those of Ti (titanium) implants (p<0.05). The 3.5 implants were implanted into the bone defects of rabbits to evaluate bone responses and 1.0 implants were implanted into the extracted sockets of rat maxilla to evaluate gingival connective tissue responses. After implantation periods, the specimens were excised and non-decalcified thin sections prepared to evaluate histological responses. After 12 weeks of implantation in the rabbit experiments, bone-to-implant contact for the Laser-Ti implants was significantly higher than for the Ti in both tibia and femoral condyle (p<0.05). Improved attachment of gingival connective tissue to the implant surface was observed for Laser-Ti and Coll/Laser-Ti in the rat maxilla. Polarized light microscopy showed perpendicular rod-like attachments of gingival collagen fibers on the Laser-Ti and Coll/ Laser-Ti implant surfaces. Ti implants had no discernible attachments with gingival connective tissue along the implant surface. In conclusion, nanosecond-pulsed laser treatment with a defocus technique produced roughened titanium surfaces with parallel grooves and micro-roughened asperities. Laser treatment of implants resulted in improved bone responses and attachment of gingival connective tissue.

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Direct attachment of fibronectin to tresyl chloride‐activated titanium

Cited by 46 publications

References 22 publications

Biological Functionalization of Dental Implants with Fibronectin : A Scanning Electron Microscopic Study

Biological Functionalization of Dental Implants with Fibronectin : A Scanning Electron Microscopic Study

Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide

Bone and Gingival Connective Tissue Responses towards Nanosecond-Pulsed Laser-Treated Titanium Implants

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