Direct association of the reticulon protein RTN1A with the ryanodine receptor 2 in neurons

Kaya, Levent; Meissner, Barbara; Riedl, Maria Christine; Muik, Martin; Schwarzer, Christoph; Ferraguti, Francesco; Sarg, Bettina; Lindner, Herbert; Schweigreiter, Rüdiger; Knaus, Hans−Günther; Romanin, Christoph; Bandtlow, Christine E.

doi:10.1016/j.bbamcr.2013.02.012

Cited by 14 publications

(15 citation statements)

References 66 publications

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“…Previous studies have demonstrated that RYR plays an important role in Purkinje cell maturation. 44 , 46 , 47 Dot blot analysis revealed that RYR levels were also upregulated in the hOEC transplantation group ( Fig. 3B ).…”

Section: Resultsmentioning

confidence: 90%

“…This improvement might protect the Purkinje cells from degeneration. On the other hand, it has been reported that increased TPH2 and RYR 44 , 46 , 47 were associated with the maturation of Purkinje cells, thus indicating that the factors might have aided in the maturation of many progenitor or immature cells. We further observed that the number of Purkinje cells increased posttransplantation with hOECs ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…32 Other studies have reported that hOEC transplantation into spinal cord injury sites facilitates neural regeneration by promoting neurite outgrowth. 50,51 Furthermore, RYR, which plays an important role in Purkinje cell differentiation and maturation, 44,46,47 was upregulated in SCA3 mice that underwent hOEC transplantation. It has been reported that in the cerebellum, RYR1 and RYR2 were expressed in Purkinje cells, while the RYR2 was expressed in granule cells.…”

Section: Discussionmentioning

confidence: 99%

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Human Olfactory Ensheathing Cell Transplantation Improves Motor Function in a Mouse Model of Type 3 Spinocerebellar Ataxia

Hsieh

Liu²,

Harn

et al. 2017

Cell Transplant

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Spinocerebellar ataxia (SCA) is a progressive neurodegenerative disease that affects the cerebellum and spinal cord. Among the 40 types of SCA, SCA type 3 (SCA3), also referred to as Machado–Joseph disease, is the most common. In the present study, we investigated the therapeutic effects of intracranial transplantation of human olfactory ensheathing cells (hOECs) in the ATXN3-84Q mouse model of SCA3. Motor function begins to decline in ATXN3-84Q transgenic mice at approximately 13 weeks of age. ATXN3-84Q mice that received intracranial hOEC transplantation into the dorsal raphe nucleus of the brain exhibited significant improvements in motor function, as measured by the rotarod performance test and footprint pattern analysis. In addition, intracranial hOEC transplantation alleviated cerebellar inflammation, prohibited Purkinje cells from dying, and enhanced the neuroplasticity of cerebellar Purkinje cells. The protein levels of tryptophan hydroxylase 2, the rate-limiting enzyme for serotonin synthesis in the cerebellum, and ryanodine receptor (RYR) increased in mice that received intracranial hOEC transplantation. Because both serotonin and RYR can enhance Purkinje cell maturation, these effects may account for the therapeutic benefits mediated by intracranial hOEC transplantation in SCA3 mice. These results indicate that intracranial hOEC transplantation has potential value as a novel strategy for treating SCA3.

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Section: Resultsmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Human Olfactory Ensheathing Cell Transplantation Improves Motor Function in a Mouse Model of Type 3 Spinocerebellar Ataxia

Hsieh

Liu²,

Harn

et al. 2017

Cell Transplant

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“…The amount and type of antibodies were the same as indicated above. In addition, we used rabbit anti-RTN1-A antibody [24], 1:10000 dilution), mouse anti-Apg-2 (Santa Cruz Biotechnology; sc-365366, 1:200 dilution) and mouse anti-Hsp105 (Santa Cruz Biotechnology; sc-74550, 1:200 dilution) antibodies for Western blotting.…”

Section: Methodsmentioning

confidence: 99%

Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress

Kern

Stanika

Sarg

et al. 2013

Biochemical Journal

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Nogo-A is the largest isoform of the Nogo/RTN4 (reticulon 4) proteins and has been characterized as a major myelin-associated inhibitor of regenerative nerve growth in the adult CNS (central nervous system). Apart from the myelin sheath, Nogo-A is expressed at high levels in principal neurons of the CNS. The specificity of Nogo-A resides in its central domain, NiG. We identified Apg-1, a member of the stress-induced Hsp110 (heat-shock protein of 110 kDa) family, as a novel interactor of NiG/Nogo-A. The interaction is selective because Apg-1 interacts with Nogo-A/RTN4-A, but not with RTN1-A, the closest paralogue of Nogo-A. Conversely, Nogo-A binds to Apg-1, but not to Apg-2 or Hsp105, two other members of the Hsp110 family. We characterized the Nogo-A–Apg-1 interaction by affinity precipitation, co-immunoprecipitation and proximity ligation assay, using primary hippocampal neurons derived from Nogo-deficient mice. Under conditions of hypoxic and oxidative stress we found that Nogo-A and Apg-1 were tightly co-regulated in hippocampal neurons. Although both proteins were up-regulated under hypoxic conditions, their expression levels were reduced upon the addition of hydrogen peroxide. Taken together, we suggest that Nogo-A is closely involved in the neuronal response to hypoxic and oxidative stress, an observation that may be of relevance not only in stroke-induced ischaemia, but also in neuroblastoma formation.

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“…Reticulon 1A also directly associates with ryanodine receptor (RyR) 2, regulating intracellular calcium buffering by reducing oscillations mediated by RyR2 on rat Purkinje neurons (Kaya et al, ). Reticulon 1A may additionally raise the surface/volume ratio of the ER favoring calcium release.…”

Section: The Structure Of the Er In Axonsmentioning

confidence: 99%

The axonal endoplasmic reticulum: One organelle—many functions in development, maintenance, and plasticity

Luarte

Cornejo

Bertin

et al. 2017

Developmental Neurobiology

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The endoplasmic reticulum (ER) is highly conserved in eukaryotes and neurons. Indeed, the localization of the organelle in axons has been known for nearly half a century. However, the relevance of the axonal ER is only beginning to emerge. In this review, we discuss the structure of the ER in axons, examining the role of ER-shaping proteins and highlighting reticulons. We analyze the multiple functions of the ER and their potential contribution to axonal physiology. First, we examine the emerging roles of the axonal ER in lipid synthesis, protein translation, processing, quality control, and secretory trafficking of transmembrane proteins. We also review the impact of the ER on calcium dynamics, focusing on intracellular mechanisms and functions. We describe the interactions between the ER and endosomes, mitochondria, and synaptic vesicles. Finally, we analyze available proteomic data of axonal preparations to reveal the dynamic functionality of the ER in axons during development. We suggest that the dynamic proteome and a validated axonal interactome, together with state-of-the-art methodologies, may provide interesting research avenues in axon physiology that may extend to pathology and regeneration. © 2017 Wiley Periodicals, Inc. Develop Neurobiol 78: 181-208, 2018.

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Direct association of the reticulon protein RTN1A with the ryanodine receptor 2 in neurons

Cited by 14 publications

References 66 publications

Human Olfactory Ensheathing Cell Transplantation Improves Motor Function in a Mouse Model of Type 3 Spinocerebellar Ataxia

Human Olfactory Ensheathing Cell Transplantation Improves Motor Function in a Mouse Model of Type 3 Spinocerebellar Ataxia

Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress

The axonal endoplasmic reticulum: One organelle—many functions in development, maintenance, and plasticity

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