Direct and Specific Detection of Glyphosate Using a Phosphatase-like Nanozyme-Mediated Chemiluminescence Strategy

Abstract

Most organophosphorus pesticide (OP) sensors reported in the literature rely on the inhibition effect of OPs on the activity of acetylcholinesterase (AChE), which suffer from the drawbacks of lack of selective recognition of OPs, high cost, and poor stability. Herein, we proposed a novel chemiluminescence (CL) strategy for the direct detection of glyphosate (an organophosphorus herbicide) with high sensitivity and specificity, which is based on the porous hydroxy zirconium oxide nanozyme (ZrO X -OH) obtained via a facile alkali solution treatment of UIO-66. ZrO X -OH displayed excellent phosphatase-like activity, which could catalyze the dephosphorylation of 3-(2′-spiroadamantyl)-4methoxy-4-(3′-phosphoryloxyphenyl)-1,2-dioxetane (AMPPD) to generate strong CL. The experimental results showed that the phosphatase-like activity of ZrO X -OH is closely related to the content of hydroxyl groups on their surface. Interestingly, ZrO X -OH with phosphatase-like properties exhibited a unique response to glyphosate because of the consumption of the surface hydroxyl group by the unique carboxyl group of glyphosates and was thus employed to develop a CL sensor for direct and selective detection of glyphosate without using bio-enzymes. The recovery for glyphosate detection of cabbage juice ranged from 96.8 to 103.0%. We believe that the as-proposed CL sensor based on ZrO X -OH with phosphatase-like properties supplies a simpler and more highly selective approach for OP assay and provides a new method for the development of CL sensors for the direct analysis of OPs in real samples.

Self-Assembled Integrated Nanozyme Cascade Biosensor with Dual Catalytic Activity for Portable Urease Analysis

“…The peak position at 2976 cm −1 corresponds to the formation of the Zr−OH bond, indicating the successful synthesis of ZrOx−OH. 27 The appearance of Zr−OH bonds could also be observed on Cu@Zr, further indicating the satisfactory loading of ZrOx−OH on Cu-BH. The peaks at 2850 and 2913 cm −1 were attributed to the stretching vibrations of the saturated C− H bond.…”

Ligand Emission-Based Turn-On Sensing of Glyphosate via Competitive Coordination with NH2-MIL-88B (Fe) MOF Nanocrystals

“…The limit of detection (LOD, calculated as 3σ/ K , where σ represents the standard deviation of the MOF (blank) emission and K is the slope of the linear calibration plot) was estimated as 198 nM. Table S1 compares the LOD values obtained in recent studies and the present work. − …”

“…The MOF structure contains Fe (2p) peaks at 724. 59 S8a. The phase and crystallinity of the MOF hardly changed before and after the addition of glyphosate.…”

Organic compound-based nanozymes for agricultural herbicide detection

“…A differentiable absorbance peak was observed at the 1 pg mL −1 border, leading us to conclude that the LOD for the system was at least 1 pg mL −1 (0.001 ng mL −1 ) for glyphosate, which implies a decent sensing performance compared to the conventional nanozyme-based sensor for glyphosate detection. 9,10,17,24,30…”