Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1

Gerganova, Veneta; Bhatia, Payal; Vincenzetti, Vincent

doi:10.1091/mbc.e20-08-0508

Cited by 4 publications

(3 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They found that Pom1p travels between clusters in a “hopping” manner to move from the cell tip toward the medial region of the cell, with these clusters creating the gradient. Additionally, they confirmed Pom1p distribution at the plasma membrane through the cycle of Pom1p gradient phosphorylation and de-phosphorylation events [ 49 ]. revealed that the PP2C phosphatase Ptc1p dephosphorylates Pom1p in vitro , and both proteins are able to form complexes in vivo .…”

Section: Mid1p-dependent Assembly Of Acr In S Pombe ...mentioning

confidence: 62%

The role of anillin/Mid1p during medial division and cytokinesis: from fission yeast to cancer cells

et al. 2022

View full text Add to dashboard Cite

Cytokinesis is the final stage of cell division cycle when cellular constituents are separated to produce two daughter cells. This process is driven by the formation and constriction of a contractile ring. Progression of these events is controlled by mechanisms and proteins that are evolutionary conserved in eukaryotes from fungi to humans. Genetic and molecular studies in different model organisms identified essential cytokinesis genes, with several conserved proteins, including the anillin/Mid1p proteins, constituting the core cytokinetic machinery. The fission yeast Schizosaccharomyces pombe represents a well-established model organism to study eukaryotic cell cycle regulation. Cytokinesis in fission yeast and mammalian cells depends on the placement, assembly, maturation, and constriction of a medially located actin-myosin contractile ring (ACR). Here, we review aspects of the ACR assembly and cytokinesis process in fission yeast and consider the regulation of such events in mammalian cells. First, we briefly describe the role of anillin during mammalian ACR assembly and cytokinesis. Second, we describe different aspects of the anillin-like protein Mid1p regulation during the S. pombe cell cycle, including its structure, function, and phospho-regulation. Third, we briefly discuss Mid1pindependent ACR assembly in S. pombe. Fourth, we highlight emerging studies demonstrating the roles of anillin in human tumourigenesis introducing anillin as a potential drug target for cancer treatment. Collectively, we provide an overview of the current understanding of medial division and cytokinesis in S. pombe and suggest the implications of these observations in other eukaryotic organisms, including humans.

show abstract

Section: Mid1p-dependent Assembly Of Acr In S Pombe ...mentioning

confidence: 62%

The role of anillin/Mid1p during medial division and cytokinesis: from fission yeast to cancer cells

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In the study of cell size dynamics, a core issue is to understand the size homeostasis strategies in various cell types, especially in fission yeast [36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51]. There are three popular phenomenological models of cell size control leading to size homeostasis [52]: (i) the timer strategy which implies a constant time between successive divisions regardless of initial size; (ii) the sizer strategy which implies cell division upon attainment of a critical size, and (iii) the adder strategy which implies a constant size addition between consecutive generations.…”

Section: Introductionmentioning

confidence: 99%

Characterizing non-exponential growth and bimodal cell size distributions in fission yeast: An analytical approach

2022

View full text Add to dashboard Cite

Unlike many single-celled organisms, the growth of fission yeast cells within a cell cycle is not exponential. It is rather characterized by three distinct phases (elongation, septation, and reshaping), each with a different growth rate. Experiments also showed that the distribution of cell size in a lineage can be bimodal, unlike the unimodal distributions measured for the bacterium Escherichia coli. Here we construct a detailed stochastic model of cell size dynamics in fission yeast. The theory leads to analytic expressions for the cell size and the birth size distributions, and explains the origin of bimodality seen in experiments. In particular, our theory shows that the left peak in the bimodal distribution is associated with cells in the elongation phase, while the right peak is due to cells in the septation and reshaping phases. We show that the size control strategy, the variability in the added size during a cell cycle, and the fraction of time spent in each of the three cell growth phases have a strong bearing on the shape of the cell size distribution. Furthermore, we infer all the parameters of our model by matching the theoretical cell size and birth size distributions to those from experimental single-cell time-course data for seven different growth conditions. Our method provides a much more accurate means of determining the size control strategy (timer, adder or sizer) than the standard method based on the slope of the best linear fit between the birth and division sizes. We also show that the variability in added size and the strength of size control in fission yeast depend weakly on the temperature but strongly on the culture medium. More importantly, we find that stronger size homeostasis and larger added size variability are required for fission yeast to adapt to unfavorable environmental conditions.

show abstract

“…Fission yeast has been an attractive model organism for several decades in studies of eukaryotic cellular growth [1][2][3][4][5][6][7]. Revealing the rules of cellular growth is crucial in understanding how size control mechanisms maintain size homeostasis in steady-state cell cultures, and this point has also been extensively studied in fission yeast [5,[7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Cell Length Growth in the Fission Yeast Cell Cycle: Is It (Bi)linear or (Bi)exponential?

et al. 2021

View full text Add to dashboard Cite

Fission yeast is commonly used as a model organism in eukaryotic cell growth studies. To describe the cells’ length growth patterns during the mitotic cycle, different models have been proposed previously as linear, exponential, bilinear and biexponential ones. The task of discriminating among these patterns is still challenging. Here, we have analyzed 298 individual cells altogether, namely from three different steady-state cultures (wild-type, wee1-50 mutant and pom1Δ mutant). We have concluded that in 190 cases (63.8%) the bilinear model was more adequate than either the linear or the exponential ones. These 190 cells were further examined by separately analyzing the linear segments of the best fitted bilinear models. Linear and exponential functions have been fitted to these growth segments to determine whether the previously fitted bilinear functions were really correct. The majority of these growth segments were found to be linear; nonetheless, a significant number of exponential ones were also detected. However, exponential ones occurred mainly in cases of rather short segments (<40 min), where there were not enough data for an accurate model fitting. By contrast, in long enough growth segments (≥40 min), linear patterns highly dominated over exponential ones, verifying that overall growth is probably bilinear.

show abstract

Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1

Cited by 4 publications

References 53 publications

The role of anillin/Mid1p during medial division and cytokinesis: from fission yeast to cancer cells

The role of anillin/Mid1p during medial division and cytokinesis: from fission yeast to cancer cells

Characterizing non-exponential growth and bimodal cell size distributions in fission yeast: An analytical approach

Cell Length Growth in the Fission Yeast Cell Cycle: Is It (Bi)linear or (Bi)exponential?

Contact Info

Product

Resources

About