Direct and indirect organogenesis of Clivia miniata and assessment of DNA methylation changes in various regenerated plantlets

Wang, Qinmei; Wang, Yuzhang; Sun, Lili; Gao, Fengzhan; Sun, Wei; He, Jing; Gao, Xiang; Wang, Li

doi:10.1007/s00299-012-1248-6

Cited by 33 publications

(13 citation statements)

References 58 publications

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“…Rapid and reversible changes in DNA methylation were also shown in potato cell suspension (Law and Suttle 2005). Lowered genomic DNA methylation of regenerants derived from shoots compared to young leaves or petals was described in case of bush lily (Clivia miniata), suggesting that the variation in methylation of regenerated plantlets was related to the nature of the explants (Wang et al 2012).…”

Section: Dna Methylation Pattern Alterationsmentioning

confidence: 89%

Plant tissue culture environment as a switch-key of (epi)genetic changes

Bednarek

Orłowska

2019

Plant Cell Tiss Organ Cult

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The in vitro tissue cultures are, beyond all difficulties, an essential tool in basic research as well as in commercial applications. Numerous works devoted to plant tissue cultures proved how important this part of the plant science is. Despite half a century of research on the issue of obtaining plants in in vitro cultures, many aspects remain unknown. The path associated with the reprogramming of explants in the fully functioning regenerants includes a series of processes that may result in the appearance of morphological, physiological, biochemical or, finally, genetic and epigenetic changes. All these changes occurring at the tissue culture stage and appearing in regenerants as tissue culture-induced variation and then inherited by generative progeny as somaclonal variation may be the result of oxidative stress, which works at the step of explant preparation, and in tissue culture as a result of nutrient components and environmental factors. In this review, we describe the current status of understanding the genetic and epigenetic changes that occur during tissue culture. Key message Variation appeared in regenerated plants as well as variation inherited by generative progeny of regenerants can may many, positive or negative impact, of gained plant materials. This review focused on factors that triggered this phenomenon with underlying oxidative stress.

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Section: Dna Methylation Pattern Alterationsmentioning

confidence: 89%

Plant tissue culture environment as a switch-key of (epi)genetic changes

Bednarek

Orłowska

2019

Plant Cell Tiss Organ Cult

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“…Culture-induced DNA methylation has been found in different species, including rice, maize, and barley [35][36][37]. Furthermore, many studies have shown that regeneration under various selection stresses or from various donor tissues induced changes in methylation patterns [38][39][40]. When uniform callus donor tissue was used in an Agrobacterium-mediated transformation procedure in rice [41,42], there was no difference in methylation among donor tissues between experiments.…”

Section: Discussionmentioning

confidence: 99%

Genetic and Global Epigenetic Modification, Which Determines the Phenotype of Transgenic Rice?

Fan

Chen

et al. 2020

IJMS

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Transgenic technologies have been applied to a wide range of biological research. However, information on the potential epigenetic effects of transgenic technology is still lacking. Here, we show that the transgenic process can simultaneously induce both genetic and epigenetic changes in rice. We analyzed genetic, epigenetic, and phenotypic changes in plants subjected to tissue culture regeneration, using transgenic lines expressing the same coding sequence from two different promoters in transgenic lines of two rice cultivars: Wuyunjing7 (WYJ7) and Nipponbare (NP). We determined the expression of OsNAR2.1 in two overexpression lines generated from the two cultivars, and in the RNA interference (RNAi) OsNAR2.1 line in NP. DNA methylation analyses were performed on wild-type cultivars (WYJ7 and NP), regenerated lines (CK, T0 plants), segregation-derived wild-type from pOsNAR2.1-OsNAR2.1 (SDWT), pOsNAR2.1-OsNAR2.1, pUbi-OsNAR2.1, and RNAi lines. Interestingly, we observed global methylation decreased in the T0 regenerated line of WYJ7 (CK-WJY7) and pOsNAR2.1-OsNAR2.1 lines but increased in pUbi-OsNAR2.1 and RNAi lines of NP. Furthermore, the methylation pattern in SDWT returned to the WYJ7 level after four generations. Phenotypic changes were detected in all the generated lines except for SDWT. Global methylation was found to decrease by 13% in pOsNAR2.1-OsNAR2.1 with an increase in plant height of 4.69% compared with WYJ7, and increased by 18% in pUbi-OsNAR2.1 with an increase of 17.36% in plant height compared with NP. This suggests an absence of a necessary link between global methylation and the phenotype of transgenic plants with OsNAR2.1 gene over-expression. However, epigenetic changes can influence phenotype during tissue culture, as seen in the massive methylation in CK-WYJ7, T0 regenerated lines, resulting in decreased plant height compared with the wild-type, in the absence of a transformed gene. We conclude that in the transgenic lines the phenotype is mainly determined by the nature and function of the transgene after four generations of transformation, while the global epigenetic modification is dependent on the genetic background. Our research suggests an innovative insight in explaining the reason behind the occurrence of transgenic plants with random and undesirable phenotypes.

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“…In vitro plant organogenesis corresponds to the formation of organs (buds, roots or shoots) from explants under controlled conditions (Sugiyama ). DNA methylation leading to changes in the chromatin state occurs in plant cells undergoing callus formation and organogenesis in vitro (Xu et al , Pischke et al , Maury et al , Wang et al ). In addition, DNA methylation in promoters participates in the establishment and/or maintenance of the undifferentiated state in plant cells (Berdasco et al ).…”

Section: Discussionmentioning

confidence: 99%

Functional characterization of Nicotiana benthamiana chromomethylase 3 in developmental programs by virus‐induced gene silencing

Hou

Lee

Hsu

et al. 2013

Physiologia Plantarum

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DNA methylation is essential for normal developmental processes and genome stability. DNA methyltransferases are key enzymes catalyzing DNA methylation. Chromomethylase (CMT) genes are specific to the plant kingdom and encode chromodomain-containing methyltransferases. However, the function of CMT genes in plants remains elusive. In this study, we isolated and characterized a CMT gene from Nicotiana benthamiana, designated NbCMT3. Alignment of the NbCMT3 amino acid sequence with other plant CMT3s showed conservation of bromo-adjacent-homology and methyltransferase catalytic domains. We investigated the expression patterns of NbCMT3 and its function in developmental programs. NbCMT3 was expressed predominately in proliferating tissues such as apical shoots and young leaves. NbCMT3 protein showed a nuclear location, which could be related to its putative cellular functions. Knocking down NbCMT3 expression by virus-induced gene silencing revealed its vital role(s) in leaf morphogenesis. The formation of palisade cells was defective in NbCMT3-silenced plants as compared with controls. NbCMT3 has a role in developmental programs.

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Direct and indirect organogenesis of Clivia miniata and assessment of DNA methylation changes in various regenerated plantlets

Abstract: Methylation variation in regenerated plantlets of C. miniata was found to be related to the types of explants, regeneration pathways and number of regeneration generations.

Cited by 33 publications

References 58 publications

Plant tissue culture environment as a switch-key of (epi)genetic changes

Plant tissue culture environment as a switch-key of (epi)genetic changes

Genetic and Global Epigenetic Modification, Which Determines the Phenotype of Transgenic Rice?

Functional characterization of Nicotiana benthamiana chromomethylase 3 in developmental programs by virus‐induced gene silencing

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