Direct and indirect effects of kisspeptin on liver oxidant and antioxidant systems in young male rats

Aydın, Mehmet; Oktar, Süleyman; Yönden, Zafer; Öztürk, Oktay Hasan; Yılmaz, Bayram

doi:10.1002/cbf.1656

Cited by 36 publications

(27 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The KP peptide is known to increase catalase activity [18], which is also neuroprotective against A β [44]. The KP peptide also has thermoregulatory effects [19] and acts via nitric oxide in the facilitation of passive avoidance learning plus memory consolidation in vivo [17].…”

Section: Resultsmentioning

confidence: 99%

“…It is therefore likely that the mechanism for neuroprotection may involve an alternative process that is more likely receptor mediated. The in vivo actions of KP peptides include actions on the opioid system [12, 13], oxytocin/vasopressin systems [4, 14, 15], neurotransmitter systems [16, 17], activation of endogenous antioxidants [18], activation of nitric oxide [17], and effects on thermoregulation [19] that could be mediated via the prostaglandin systems [46, 47]. …”

Section: Discussionmentioning

confidence: 99%

“…However, in vivo actions on the opioid system [12, 13], oxytocin/vasopressin systems [4, 14, 15], neurotransmitter systems [16, 17], activation of endogenous antioxidants [18], activation of nitric oxide [17], and possible activation of prostaglandin synthesis [19] have not been tested with GPR-54 or NPFF receptor antagonists.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Role of Neurotransmitters in Protection against Amyloid-β Toxicity by KiSS-1 Overexpression in SH-SY5Y Neurons

Chilumuri

Milton

2013

ISRN Neuroscience

View full text Add to dashboard Cite

Recent studies have suggested that the kisspeptin (KP) and kissorphin (KSO) peptides have neuroprotective actions against the Alzheimer's amyloid-β (Aβ) peptide. Overexpression of the human KiSS-1 gene that codes for KP and KSO peptides in SH-SY5Y neurons has also been shown to inhibit Aβ neurotoxicity. The in vivo actions of KP include activation of neuroendocrine and neurotransmitter systems. The present study used antagonists of KP, neuropeptide FF (NPFF), opioids, oxytocin, estrogen, adrenergic, cholinergic, dopaminergic, serotonergic, and γ-aminobutyric acid (GABA) receptors plus inhibitors of catalase, cyclooxygenase, nitric oxide synthase, and the mitogen activated protein kinase cascade to characterize the KiSS-1 gene overexpression neuroprotection against Aβ cell model. The results showed that KiSS-1 overexpression is neuroprotective against Aβ and the action appears to involve the KP or KSO peptide products of KiSS-1 processing. The mechanism of neuroprotection does not involve the activation of the KP or NPFF receptors. Opioids play a role in the toxicity of Aβ in the KiSS-1 overexpression system and opioid antagonists naloxone or naltrexone inhibited Aβ toxicity. The mechanism of KiSS-1 overexpression induced protection against Aβ appears to have an oxytocin plus a cyclooxygenase dependent component, with the oxytocin antagonist atosiban and the cyclooxygenase inhibitor SC-560 both enhancing the toxicity of Aβ.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Role of Neurotransmitters in Protection against Amyloid-β Toxicity by KiSS-1 Overexpression in SH-SY5Y Neurons

Chilumuri

Milton

2013

ISRN Neuroscience

View full text Add to dashboard Cite

show abstract

“…Therefore, measuring total oxidant and antioxidant capacities and making comparisons are much in use and practical today. Even TAC and TOC can be used in monitoring the treatment in use for a given disease or experimental study [44,45] . In the present study, TOC increased while TAC decreased in CCl 4 given animals as compared to the control.…”

Section: Discussionmentioning

confidence: 99%

Salgı

Karaman¹,

Özen²,

Dağ³

et al. 2016

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Omega-3 is a polyunsaturated fatty acid known to have immunomodulatory functions. In the present study, ameliorative potential of omega-3 in experimental carbon tetrachloride (CCl4) toxicity was investigated. Total of 40 adult male Wistar albino rats were allocated into five groups and were subcutaneously given once every two days for 6 weeks the followings: Group 1 (Control): 0.5 ml/kg serum physiologic, Group 2 (Omega): 0.5 g/kg omega-3, Group 3 (Vehicle): 0.5 ml/kg pure olive oil, Group 4 (CCl4): 0.5 ml/kg CCl4, Group 5 (CCl4 + Omega): 0.5 ml/kg CCl4 plus 0.5 g/kg omega-3. At the end of the treatments, blood samples were collected and necropsy was performed for collection of liver tissues. Serum AST, AlT, GGT, TAC, TOC, triglyceride, and visfatin levels were detected. liver morphology and immunoreactivities against TGF-α, TGF-β, PPAR-α, and PPAR-γ were assessed. Serum AST, AlT, GGT, and TOC levels significantly increased while TAC level decreased in CCl4 given animals as compared to the control group. No significant changes were observed in triglyceride and visfatin levels. Immunohistochemical staining revealed increased TGF-α and TGF-β expressions and decreased PPAR-α and PPAR-γ expressions in liver of CCl4 given animals. Omega-3 supplementation has prominent effects in correcting the biochemical and immunohistochemical parameters studied as well as the tissue morphology. The results of the investigation indicated that omega-3 has ameliorative effects on the oxidative tissue degeneration and inflammatory processes induced by CCl4 treatment in rats. Keywords: CCl4, Omega-3, TGF-α, TGF-β, PPAR-α, PPAR-γ Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi ÖzetOmega-3 bağışıklığı düzenleyici fonksiyonları olduğu bilinen çoklu doymamış bir yağ asididir. Bu çalışmada, deneysel karbon tetraklorür (CCl4) toksikasyonunda omega-3'ün koruyucu potansiyel etkisi araştırılmıştır. Toplam 40 adet ergin erkek Wistar albino rat eşit beş gruba ayrıldı ve 6 hafta süresince her iki günde bir olmak üzere subkutan yolla deneklere tarif edilen uygulamalar yapıldı; Grup 1 (kontrol): 0.5 ml/kg serum fizyolojik, Grup 2 (Omega): 0.5 g/kg omega-3, Grup 3 (Taşıt): 0.5 ml/kg saf zeytin yağı, Grup 4 (CCl4): 0.5 ml/kg CCl4, Grup 5 (CCl4 + Omega): 0.5 ml/kg CCl4 artı 0.5 g/kg omega-3. Araştırma süresinin sonunda, kan örnekleri alınan deneklere karaciğer doku örneklerinin toplanması amacıyla nekropsi uygulandı. Serum AST, AlT, GGT, TAC, TOC, trigliserid ve visfatin seviyeleri belirlendi. Karaciğer morfolojisi incelendi ve TGF-α, TGF-β, PPAR-α ve PPAR-γ immunreaktiviteleri tespit edildi. Kontrol grubu ile karşılaştırıldığında CCl4 verilen hayvanlarda serum AST, AlT, GGT ve TOC seviyelerinin anlamlı derecede arttığı, TAC seviyesinin ise azaldığı gözlemlendi. Trigliserid ve visfatin seviyelerinde anlamlı bir değişikliğin olmadığı belirlendi. İmmunohistokimyasal boyamalarda CCl4 verilen hayvanlarda TGF-α ve TGF-β immunreaktiviteleri artarken PPAR-α ve PPAR-γ immunreaktivitelerinde azalma dikkati çekti. Omega-3 takviyesinin incel...

show abstract

“…In rodents, the expression of kiss-I gene might relate to the increase of body weight and change with the high fat diets (Moral et al, 2011; Quennell et al, 2011). Kisspeptin might directly reduce the hepatic lipid peroxidation, preventing the negative effect of lipid peroxidation on intracellular regulatory mechanisms managing the release of hypothalamic hormones leading to defective neurotransmission (Aydin et al, 2010). However, these observations suggested that there is a relationship between kisspeptin and lipid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Effects of Kisspeptin-10 on Lipid Metabolism in Cultured Chicken Hepatocytes

Wu¹,

Fu²,

Huang³

et al. 2012

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

Our previous studies showed that kisspeptin-10 (Kp-10) injected in vivo can markedly increase lipid anabolism in liver of quails. In order to investigate the direct effect of Kp-10 on lipid metabolism of hepatocytes in birds, cells were separated from embryos livers and cultured in vitro with 0, 100 and 1,000 nM Kp-10, respectively. The results showed that after 24 h treatment, cells viability was not affected by 100 nM Kp-10, but showed a mild decrease with 1,000 nM Kp-10 compared to the control cells. Based on the results of the cell viability, 100 nM dosage of Kp-10 was selected for the further study and analysis. Compared with control cells, total cholesterol (Tch) contents in 100 nM treated cells were increased by 51.23%, but did not reach statistical significance, while the level of triglyceride (TG), high density of lipoprotein-cholesterol (HDL-C) and low density of lipoprotein-cholesterol (LDL-C) were significantly increased. Real-time PCR results showed that ApoVLDL-II mRNA expression had a tendency to increase, genes including sterol regulatory element-binding protein-1 (SREBP-1), acetyl coenzyme A carboxylase α (ACCα), carnitine palmitoyltransferase 1 (CPT1), 3-hydroxyl-3-methylglutaryl-coenzyme A reductases (HMGCR) and stearyl coenzyme A dehydrogenase-1 (SCD1) mRNA in hepatocytes were significantly down-regulated by 100 nM Kp-10. However, contrary to its gene expression, SREBP-1 protein expression was significantly up-regulated by 100 nM Kp-10. Some of the significant correlations in mRNA expression were found between genes encoding hepatic factors or enzymes involved in lipid metabolism in liver of birds. These results indicate that Kp-10 stimulates lipid synthesis directly in primary cultured hepatocytes of chickens.

show abstract

Direct and indirect effects of kisspeptin on liver oxidant and antioxidant systems in young male rats

Cited by 36 publications

References 62 publications

The Role of Neurotransmitters in Protection against Amyloid-β Toxicity by KiSS-1 Overexpression in SH-SY5Y Neurons

The Role of Neurotransmitters in Protection against Amyloid-β Toxicity by KiSS-1 Overexpression in SH-SY5Y Neurons

Salgı

Effects of Kisspeptin-10 on Lipid Metabolism in Cultured Chicken Hepatocytes

Contact Info

Product

Resources

About