Direct and heritable effects of natural tidal environments on DNA methylation in Pacific oysters (Crassostrea gigas)

Wang, Xinxing; Li, Ao; Wang, Wei; Zhang, Guofan; Li, Li

doi:10.1016/j.envres.2021.111058

Cited by 16 publications

(23 citation statements)

References 46 publications

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“…Bisulfite conversion efficiencies reached 99.9% in all analyzed samples. The average DNA methylation levels ranged from 0.11 to 0.14 (Fig 1A), consistent with previous studies [26, 27]. Compared with public WGBS data of C. gigas [21, 22, 26, 27].…”

Section: Resultssupporting

confidence: 90%

“…The whole-genome bisulfite sequencing (WGBS) was then performed using muscle tissues from both parents, three diploid and three triploid offspring individuals in each [26,27]. Compared with public WGBS data of C. gigas [21,22,26,27]. high Pearson correlation coefficients (average r = 0.858) were found between our sequencing data and public datasets (S1C Fig) . In all, the WGBS data quality in this study is solid.…”

Section: Globally Dna Methylation Landscape Of C Gigasmentioning

confidence: 89%

“…To profile the inheritance patterns of DNA methylation in C. gigas, regular F 1 diploid and triploid oysters were produced by crossing a normal diploid male and female oysters in two independent families. The whole-genome bisulfite sequencing (WGBS) was then performed using muscle tissues from both parents, three diploid and three triploid offspring individuals in each [26,27]. Compared with public WGBS data of C. gigas [21,22,26,27].…”

Section: Globally Dna Methylation Landscape Of C Gigasmentioning

confidence: 99%

“…phylotypic plasticity [26,27]. While the previous study hypothesized that intergenerational inheritance in DNA methylation exists in C. gigas [25], more evidence is needed to make general collusions.…”

Section: Introductionmentioning

confidence: 97%

“…Because of the ecological and economic values, C. gigas has the most extensive DNA methylation studies in Molluscs [11]. These works are primarily concerned with gene expression regulation [21-23], development processes [24, 25], phylotypic plasticity [26, 27]. While the previous study hypothesized that intergenerational inheritance in DNA methylation exists in C. gigas [25], more evidence is needed to make general collusions.…”

Section: Introductionmentioning

confidence: 99%

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Intergenerational transfer and sex differences of DNA methylation patterns in the Pacific oyster (Crassostrea gigas)

Teng

et al. 2022

Preprint

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Apart from DNA-sequence-based inheritance, inheritance of epigenetic marks such as DNA methylation is controversial across the tree of life. In mammals, post-fertilization and primordial germ cell reprogramming processes erased most parental DNA methylation information. In nonmammalian vertebrates and insects, it has been proposed that DNA methylation is an essential hereditary carrier. However, how and to what extent general DNA methylation reprogramming affects intergenerational inheritance in molluscs remains unclear. Here, we investigated genome-wide DNA methylation in a mollusc model, the Pacific oyster (Crassostrea gigas), to test how epigenetic information transfers from parents to offspring. Analysis of global methylome revealed that the DNA methylation patterns are highly conserved within families. Almost half of the differentially methylated CpG dinucleotides (DMCs) between families in parents could transfer to offspring. These results provided the direct evidence for the hypothesis that the Pacific oyster DNA methylation patterns are inherited in generations. Moreover, distinct DNA methylation differences between male and female somatic tissues in C. gigas are revealed in this study. These sex-differential methylated genes significantly enriched in the regulation of Rho protein signal transduction process, which indicated that DNA methylation might have an essential role in the sexual differentiation of somatic tissues in C. gigas.

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