Direct Analysis of Underivatized Amino Acids in Plant Extracts by LC-MS-MS

Thiele, Björn; Stein, Nadine; Oldiges, Marco; Hofmann, Diana

doi:10.1007/978-1-61779-445-2_25

Cited by 26 publications

(14 citation statements)

References 29 publications

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“…High-performance liquid chromatography (HPLC) was performed as previously described ( 13 ). Briefly, HPLC was performed using the LC-9A liquid chromatograph system (Shimadzu Corporation, Kyoto, Japan).…”

Section: Methodsmentioning

confidence: 99%

Effect of early weaning on the expression of excitatory amino acid transporter 1 in the jejunum and ileum of piglets

Wang

Cui

Zhang

et al. 2017

Molecular Medicine Reports

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The present study aimed to compare the expression levels of excitatory amino acid transporters (EAATs) and growth status of piglets weaned at 10–20 days after birth with suckling piglets. A total of 40 hybrid piglets (Landrace × Large White × Duroc) born to 40 different sows, with similar body weight were selected for the present study. They were randomly divided into two groups (n=20 per group): Control group (suckling piglets) and experimental group (weaned piglets, reared in isolation). The experiment lasted for 10 days. At the end of the experiment, 12 piglets were randomly selected from each group and the jejunum and the ileum were collected in order to determine excitatory amino acid carrier 1 (EAAC1) expression levels and free amino acid content. The present study determined that early weaning significantly reduced EAAC1 gene and protein (57 and 73 kDa) expression levels and glutamate transporter associate protein 3–18 (GTRAP3-18; 50 kDa) in the jejunum and the ileum compared with the suckling group (P<0.05). Weaning led to an increased content of free glutamic acid (Glu) and total amino acids in the jejunum; however, content of free Glu and total amino acids in the ileum was significantly reduced (P<0.05). Early weaning reduced the expression of EAAC1 and GTRAP3-18, which was possibly due to the amino acid absorption and transport disorder in the small intestine due to the Glu deficiency.

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Section: Methodsmentioning

confidence: 99%

Effect of early weaning on the expression of excitatory amino acid transporter 1 in the jejunum and ileum of piglets

Wang

Cui

Zhang

et al. 2017

Molecular Medicine Reports

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show abstract

“…Sample extraction for the analysis of AA analysis was conducted following Thiele et al [14]. A 0.5 mL aliquot of 0.5 M aqueous HCL-ethanol (1:1, v/v) as solvent was added to 100 g of dried and ground material, then the extract was centrifuged at 12,000g for 15 min at 4 ºC, and the supernatant was separated directly and used for analysis.…”

Section: Amino Acids By Lcms/msmentioning

confidence: 99%

A Comparison of Nutritional Values, Bioactive Compounds, Amino Acids, and Antioxidant Activities of Alfalfa (Medicago sativa) Plant and Pellet for Use as Beneficial Material Ruminant Feed

Srisaikham

2021

Walailak J Sci & Tech

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Aqueous extracts of whole alfalfa plants (AP) and alfalfa pellets (APe) were examined for nutritional values, bioactive compounds (total phenolic content (TPC), phenolic compounds (PC), total flavonoids content (TFC), flavonoids compounds (FC), isoflavones), amino acids (AA) and antioxidant activity of AP and APe assessed via a DHHP radical scavenging assay and ferric reducing antioxidant power (FRAP) assay. The potential ingredients in the form of AP and APe were used to evaluate the nutritional values measured by chemical composition, and to investigate bioactive compounds, AA and antioxidant activity to further contribute to use as a feed ingredient or application as a high-value alternative feed supplement in the ruminant diet. Crude protein and tannins were higher in the APe (18.17 and 3.08 %) than the AP (16.21 and 1.80 %). Higher content of organic matter, crude fiber, neutral-detergent fiber, acid-detergent fiber, acid-detergent lignin was observed for AP than APe. The TPC and TFC were more abundant in AP than in APe and AP also showed stronger antioxidant activity via DPPH than APe, with positive correlations of TPC, TFC and DPPH. The highest values of gallic acid in terms of PC and apigenin in terms of FC of APe were 215.30 and 2278.27 µg/g dry weight (DW), respectively, while the highest levels of genistein and daidzein occurred in AP at 104.37 and 130.91 µg/g DW, respectively. The total of essential AA was found to be in vary the range of 9.33 to 16689.47 µg/g DW in different forms of samples, with valine as the highest (5451 µg/g DW) in APe, followed by phenylalanine (4.30 µg/g DW) in AP.

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“…The liquid chromatography-mass spectrometry (LC-MS) with atmospheric pressure chemical ionization (APCI) in positive mode was used by Ö zcan and Ş enyuva (2006) for the determination of amino acid in several foodstuffs including different fruit juices, baby food, milk, beer, wine and nuts. Another methods applying the liquid chromatography-tandem mass spectrometry (LC-MS/MS) with positive electrospray ionization (ESI) were described for analysis of amino acids in plant extracts (Thiele et al 2012), orange juices (Gómez-Ariza et al 2005) and bakery products (Ciesarová et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Free amino acids profile of Polish and Slovak honeys based on LC–MS/MS method without the prior derivatisation

et al. 2017

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LC-MS/MS method was applied for determination of free amino acids in honey without derivatisation steps. Twenty free amino acids including aspartic acid, asparagine, glutamic acid, glutamine, alanine, arginine, glycine, leucine, histidine, hydroxyproline, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, valine and ornithine were analyzed in thirty honey samples from Poland and Slovakia. The analysis covered: acacia, lime, rape, multifloral and forest types of honey. Applied method was characterized to had good sensitivity with limit of detection ranged from 3.0 ng/cm for valine to 13.0 ng/cm for hydroxyproline. Average content of proline (main amino acid component in honey) ranged from 151.46 μg/g (rape honey from Slovakia) to 389.66 μg/g for forest honey (honeydew honey) from Poland. In analyzed honeys large quantities of glutamine, glutamic acid, lysine, phenylalanine, asparagine, alanine, and valine were also found.

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Direct Analysis of Underivatized Amino Acids in Plant Extracts by LC-MS-MS

Cited by 26 publications

References 29 publications

Effect of early weaning on the expression of excitatory amino acid transporter 1 in the jejunum and ileum of piglets

Effect of early weaning on the expression of excitatory amino acid transporter 1 in the jejunum and ileum of piglets

A Comparison of Nutritional Values, Bioactive Compounds, Amino Acids, and Antioxidant Activities of Alfalfa (Medicago sativa) Plant and Pellet for Use as Beneficial Material Ruminant Feed

Free amino acids profile of Polish and Slovak honeys based on LC–MS/MS method without the prior derivatisation

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