2013
DOI: 10.1002/rcm.6766
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Direct analysis of sterols by derivatization matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and tandem mass spectrometry

Abstract: Suitable matrices for picolinyl esters are DHB and THAP, for N-methylpyridyl ethers THAP, and for sulphated esters p-nitroaniline and dithranol. Using cholesterol, the limits of detection (LODs) for sulphated esters were 0.2 µg/mL and for picolinyl esters, 1.5 µg/mL. N-Methylpyridyl ethers were found unsuitable for sterol quantitation.

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Cited by 33 publications
(27 citation statements)
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“…Other applications of MALDI TOF/TOF were also reported for carbohydrates and peptides [35, 36]. Whereas fatty acids have been investigated by MALDI TOF/TOF and their CID spectra are well understood, steroids and their conjugates have been far less investigated [37]. Here, we demonstrate, for the first time to our knowledge, the application of MALDI TOF/TOF instrumentation to induce CRF and enable structural characterization of sulfated steroids.…”
Section: Introductionmentioning
confidence: 65%
“…Other applications of MALDI TOF/TOF were also reported for carbohydrates and peptides [35, 36]. Whereas fatty acids have been investigated by MALDI TOF/TOF and their CID spectra are well understood, steroids and their conjugates have been far less investigated [37]. Here, we demonstrate, for the first time to our knowledge, the application of MALDI TOF/TOF instrumentation to induce CRF and enable structural characterization of sulfated steroids.…”
Section: Introductionmentioning
confidence: 65%
“…Likewise, long-chain alkenones and the corresponding U In addition to stratigraphic analysis of the biomarker record in sediments, LDI FTICR-MS may open new avenues for the examination of modern, spatially organized ecosystems, such as redox boundaries in sediments or microbial mats, where lipid biomarker and pigment analysis could reveal ultra-fine community composition. Several of these potentially interesting molecular targets for LDI FTICR-MS will require intense methodological development and possibly demand application of artificial matrix or derivatization techniques [e.g., sterols (67)], but could reward us with a transformative insight into the biomarker world.…”
Section: Resultsmentioning
confidence: 99%
“…MALDI-MS [15,16] has been tested for its applicability as a rapid screening technique. The challenges in MALDI-MS analysis of hydrophobic molecules such as cholesterol and its ester, steroids and coenzyme Q10 stem from the complexity and low ionization efficiencies of the target compounds.…”
Section: Introductionmentioning
confidence: 99%