Direct analysis of [6,6-2H2]glucose and [U-13C6]glucose dry blood spot enrichments by LC–MS/MS

Coelho, Margarida; Mendes, Vera M.; Lima, Inês S.; Martins, Fátima O.; Fernandes, Ana B.; Macedo, M. Paula; Jones, John G.; Manadas, Bruno

doi:10.1016/j.jchromb.2016.04.027

Cited by 4 publications

(5 citation statements)

References 22 publications

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“…Multiple reaction monitoring (MRM) is an LC-MS/MS approach that has been used to identify [6,6 2H2] glucose and [U-13C6] glucose enrichment using DBS without prior derivatization. The obtained results prove that DBS can contribute significantly to the study and diagnosis of complex metabolic diseases such as type 2 diabetes [60].…”

Section: Metabolite Detectionmentioning

confidence: 54%

Dried Blood Spot in Laboratory: Directions and Prospects

et al. 2020

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Over the past few years, dried blood spot (DBS) technology has become a convenient tool in both qualitative and quantitative biological analysis. DBS technology consists of a membrane carrier (MC) on the surface of which a biomaterial sample becomes absorbed. Modern analytical, immunological or genomic methods can be employed for analysis after drying the sample. DBS has been described as the most appropriate method for biomaterial sampling due to specific associated inherent advantages, including the small volumes of biomaterials required, the absence of a need for special conditions for samples’ storage and transportation, improved stability of analytes and reduced risk of infection resulting from contaminated samples. This review illustrates information on the current state of DBS technology, which can be useful and helpful for biomedical researchers. The prospects of using this technology to assess the metabolomic profile, assessment, diagnosis of communicable diseases are demonstrated.

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Section: Metabolite Detectionmentioning

confidence: 54%

Dried Blood Spot in Laboratory: Directions and Prospects

et al. 2020

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“…The collision energy was set at −14 eV and declustering potential at −50 eV for all transitions. This method has been previously validated for analysis of [U‐ 13 C]‐ and [6,6‐ 2 H]glucose enrichments in DBSs of rats administered with these tracers 19 …”

Section: Methodsmentioning

confidence: 99%

“…Blood was harvested without anticoagulants. A few microliters of blood was placed on 903 Protein Saver Snap Apart Card filter paper (Whatman, GE Healthcare Ltd, Cardiff, UK), which was then dried at room temperature and stored in a desiccator 19 with the dried blood spots (DBSs) subjected to gas chromatography MS (GC–MS) and liquid chromatography coupled to tandem MS (LC–MS/MS) analyses. The remaining blood was centrifuged at 2862 × g for 5 min at 4°C to eliminate the cell debris.…”

Section: Methodsmentioning

confidence: 99%

“…This method has been previously validated for analysis of [U- 13 C]- and [6,6- 2 H]glucose enrichments in DBSs of rats administered with these tracers. 19 …”

Section: Methodsmentioning

confidence: 99%

“…This method has been previously validated for analysis of [U- 13 C]-and [6,6-2 H]glucose enrichments in DBSs of rats administered with these tracers. 19 Peak area ratios were calculated from integrated peak areas of the analyte and internal standard on MultiQuant 2.1.1 (AB Sciex). Quantification was performed for glucose M + 0, M + 2, M + 4, and M + 6, by monitoring the sum of the transitions of each glucose species (Table S1).…”

Section: Lc-ms/ms Of Dbss and Data Analysismentioning

confidence: 99%

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Enrichment of hepatic glycogen and plasma glucose from H₂¹⁸O informs gluconeogenic and indirect pathway fluxes in naturally feeding mice

et al. 2022

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Deuterated water ( 2 H 2 O) is a widely used tracer of carbohydrate biosynthesis in both preclinical and clinical settings, but the significant kinetic isotope effects (KIE) of 2 H can distort metabolic information and mediate toxicity. 18 O-water (H 2 18 O) has no significant KIE and is incorporated into specific carbohydrate oxygens via well-defined mechanisms, but to date it has not been evaluated in any animal model. Mice were given H 2 18 O during overnight feeding and 18 O-enrichments of liver glycogen, triglyceride glycerol (TG), and blood glucose were quantified by 13 C NMR and mass spectrometry (MS). Enrichment of oxygens 5 and 6 relative to body water informed indirect pathway contributions from the Krebs cycle and triose phosphate sources. Compared with mice fed normal chow (NC), mice whose NC was supplemented with a fructose/glucose mix (i.e., a high sugar [HS] diet) had significantly higher indirect pathway contributions from triose phosphate sources, consistent with fructose glycogenesis. Blood glucose and liver TG 18 O-enrichments were quantified by MS. Blood glucose 18 O-enrichment was significantly higher for HS versus NC mice and was consistent with gluconeogenic fructose metabolism. TG 18 O-enrichment was extensive for both NC and HS mice, indicating a high turnover of liver triglyceride, independent Abbreviations used: 2 H 2 O, water enriched with deuterium; DBS, dried blood spot; GC-MS, gas chromatography mass spectrometry; H 2 18 O, water enriched with oxygen-18; HS, chow supplemented with fructose/glucose mix (i.e., a high sugar diet); KIE, kinetic isotope effects; LC-MS/MS, liquid chromatography coupled to tandem mass spectrometry; MAG, monoacetone glucose; NC, normal chow; TAMAG, 3,5,6-tri-O-[1-13 C]acetyl monoacetone glucose. Margarida Coelho and Rohit Mahar are joint first authors.

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Colorimetric/SERS dual-mode sensing of glucose based on cascade reaction of peroxidase-like Au@Pt/Fe-DACDs nanozyme

Wang,

Liang,

et al. 2024

J Mater Sci

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Direct analysis of [6,6-2H2]glucose and [U-13C6]glucose dry blood spot enrichments by LC–MS/MS

Cited by 4 publications

References 22 publications

Dried Blood Spot in Laboratory: Directions and Prospects

Dried Blood Spot in Laboratory: Directions and Prospects

Enrichment of hepatic glycogen and plasma glucose from H₂¹⁸O informs gluconeogenic and indirect pathway fluxes in naturally feeding mice

Colorimetric/SERS dual-mode sensing of glucose based on cascade reaction of peroxidase-like Au@Pt/Fe-DACDs nanozyme

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Direct analysis of [6,6-2H2]glucose and [U-13C6]glucose dry blood spot enrichments by LC–MS/MS

Cited by 4 publications

References 22 publications

Dried Blood Spot in Laboratory: Directions and Prospects

Dried Blood Spot in Laboratory: Directions and Prospects

Enrichment of hepatic glycogen and plasma glucose from H₂18O informs gluconeogenic and indirect pathway fluxes in naturally feeding mice

Colorimetric/SERS dual-mode sensing of glucose based on cascade reaction of peroxidase-like Au@Pt/Fe-DACDs nanozyme

Contact Info

Product

Resources

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Enrichment of hepatic glycogen and plasma glucose from H₂¹⁸O informs gluconeogenic and indirect pathway fluxes in naturally feeding mice