Direct analysis in real time (DART) mass spectrometry of nucleotides and nucleosides: elucidation of a novel fragment [C5H5O]+and its in-source adducts

Curtis, Matthew; Minier, Mikael A.; Chitranshi, Priyanka; Sparkman, O. David; Jones, Patrick R.; Xue, Liang

doi:10.1016/j.jasms.2010.03.046

Cited by 45 publications

(17 citation statements)

References 32 publications

(39 reference statements)

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“…Interestingly, in-source adducts after fragmentation were observed during analysis of nucleotides by DART MS [30]. In the present paper, high numbers of ISD fragments and complexes produced from monosaccharides and disaccharides were observed for the first time during our investigation of the feasibility of detecting oligosaccharides using DART MS.…”

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confidence: 51%

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Differentiation of Disaccharide Isomers by Temperature-Dependent In-Source Decay (TDISD) and DART-Q-TOF MS/MS

Yang

Shi

Yao

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. Helium direct analysis in real time (He-DART) mass spectrometry (MS) of some compounds, polysaccharides, for example, usually tends to be challenging because of the occurrence of prominent in-source decay (ISD), which was considered as an undesired side reaction, as it complicated the resulting mass spectra. Our approach is to take advantage of an efficient and practical method termed the temperature-dependent ISD (TDISD) technique combined with fragmentation of the dehydrated dimers using DART Q-TOF tandem mass spectrometry for differentiation of disaccharide isomers. In this study, cross-ring cleavages and non-ovalent complexes were detected in the spectra of the saccharides. It was observed that the gas heater temperature had a significant effect on the absence or presence of signal in DART spectra. At high gas temperature, ions in high mass region began to appear. Based on the types of crossring cleavages and noncovalent complexes, disaccharide isomers with different linkage positions can be differentiated in both positive and negative ion modes at a lower DART gas temperature. Additionally, anomeric configurations were assigned on the basis of the relative abundance ratio of m/z 198:342 obtained by the comparison of the positive ion mode tandem mass spectrum of an α isomer dimer generated at higher DART gas temperature and that of the corresponding β one. In general, this method is easy, fast, effective, and robust for identifying disaccharide isomers.

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confidence: 51%

“…Even if DART is generally considered as a soft ionization process, a significant degree of in-source decay (ISD) fragmentation can occur during the ionization event [6,29,30], especially when using He as DART gas. Interestingly, in-source adducts after fragmentation were observed during analysis of nucleotides by DART MS [30].…”

mentioning

confidence: 99%

Differentiation of Disaccharide Isomers by Temperature-Dependent In-Source Decay (TDISD) and DART-Q-TOF MS/MS

Yang

Shi

Yao

et al. 2015

J. Am. Soc. Mass Spectrom.

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“…For example, TAGs have been detected from olive oil desorbed from a glass rod, with a ten-fold increase in signal observed by doping ammonia into the gas stream to facilitate [M+NH 4 ] + ion formation [241]. However, desorption/ionization of TAGs resulted in significant fragmentation, a phenomenon not uncommon in DART and one that generally increases with gas temperature [245,246]. This leads to formation of DAG-like ions with greater abundance than ions arising from intact TAG molecules, thereby reducing sensitivity and making…”

Section: Direct Analysis In Real Time (Dart)mentioning

confidence: 99%

Surface analysis of lipids by mass spectrometry: More than just imaging

Ellis

Brown

Panhuis

et al. 2013

Progress in Lipid Research

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Mass spectrometry is now an indispensable tool for lipid analysis and is arguably the driving force in the renaissance of lipid research. In its various forms, mass spectrometry is uniquely capable of resolving the extensive compositional and structural diversity of lipids in biological systems. Furthermore, it provides the ability to accurately quantify molecular-level changes in lipid populations associated with changes in metabolism and environment; bringing lipid science to the "omics" age. The recent explosion of mass spectrometry-based surface analysis techniques is fuelling further expansion of the lipidomics field. This is evidenced by the numerous papers published on the subject of mass spectrometric imaging of lipids in recent years. While imaging mass spectrometry provides new and exciting possibilities, it is but one of the many opportunities direct surface analysis offers the lipid researcher. In this review we describe the current state-ofthe-art in the direct surface analysis of lipids with a focus on tissue sections, intact cells and thin-layer chromatography substrates. The suitability of these different approaches towards analysis of the major lipid classes along with their current and potential applications in the field of lipid analysis are evaluated. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 2 AbstractMass spectrometry is now an indispensable tool for lipid analysis and is arguably the driving force in the renaissance of lipid research. In its various forms, mass spectrometry is uniquely capable of resolving the extensive compositional and structural diversity of lipids in biological systems. Furthermore, it provides the ability to accurately quantify molecular-level changes in lipid populations associated with changes in metabolism and environment;bringing lipid science to the "omics" age. The recent explosion of mass spectrometry-based surface analysis techniques is fuelling further expansion of the lipidomics field. This is evidenced by the numerous papers published on the subject of mass spectrometric imaging of lipids in recent years. While imaging mass spectrometry provides new and exciting possibilities, it is but one of the many opportunities direct surface analysis offers the lipid researcher. In this review we describe the current state-of-the-art in the direct surface analysis of lipids with a focus on tissue sections, intact cells and thin-layer chromatography substrates.The suitability of these different approaches towards analysis of the major lipid classes along with their current and potential applications in the field of lip...

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“…In the studies described above, [ClMg(κ 2 ‐O 2 C)] − was formed by dissociation of an oxalate complex produced by electrospray ionization:

true [ClMgC_{2} O_{4}]^{-} \to [ClMg (κ^{2} - normalO_{2} normalC)]^{-} + CO_{2}

…”

Section: Introductionmentioning

confidence: 99%

C−C Bond Formation of Mg‐ and Zn‐Activated Carbon Dioxide

Miller

Uggerud

2018

Chemistry A European J

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Gas-phase activation of CO by chloride tagged metal atoms, [ClM] (M=Mg, Zn), has been investigated by mass spectrometry and high-level quantum chemistry. Both metals activate CO with significant bending of the CO moiety to form complexes with the general formula [ClM,CO ] . The structure of the metal-CO complex depends on the method of formation, and the energy landscapes and reaction dynamics have been probed by collisional induced dissociation and thermal ion molecule reactions with isotopically labeled species. Having established these structural relationships, the gas-phase reactivity of [ClM(κ -O C)] with acetaldehyde (here considered a carbohydrate mimic) was then studied. Formation of lactate and enolate-pyruvate complexes are observed, showing that CO fixation by C-C bond formation takes place. For M=Zn, even formation of free pyruvate ([C H O ] ) is observed. Implications of the observed CO reactivity for the electrochemical conversion of carbon dioxide, and to biochemical and artificial photosynthesis is briefly discussed. Detailed potential energy diagrams obtained by the quantum chemical calculations offer models consistent with experimental observation.

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Direct analysis in real time (DART) mass spectrometry of nucleotides and nucleosides: elucidation of a novel fragment [C₅H₅O]⁺and its in-source adducts

Cited by 45 publications

References 32 publications

Differentiation of Disaccharide Isomers by Temperature-Dependent In-Source Decay (TDISD) and DART-Q-TOF MS/MS

Differentiation of Disaccharide Isomers by Temperature-Dependent In-Source Decay (TDISD) and DART-Q-TOF MS/MS

Surface analysis of lipids by mass spectrometry: More than just imaging

C−C Bond Formation of Mg‐ and Zn‐Activated Carbon Dioxide

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