Direct actions of ACTH on ovarian function of pseudopregnant rabbits

Guelfi, Gabriella; Zerani, Massimo; Brecchia, Gabriele; Parillo, Francesco; Dall’Aglio, Cecilia; Maranesi, Margherita; Boiti, Cristiano

doi:10.1016/j.mce.2011.03.017

Cited by 18 publications

(13 citation statements)

References 48 publications

(8 reference statements)

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“…The alteration of LH levels may influence progesterone secretion in CL. Furthermore, studies suggested that ACTH or cortisol could directly regulate formation and function of CL (40). However, the mechanism has not been determined.…”

Section: Discussionmentioning

confidence: 99%

Exogenous adrenocorticotropic hormone affects genome‐wide DNA methylation and transcriptome of corpus luteum in sows

Zhao

Wei

et al. 2018

FASEB j.

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Stress is known to cause corpus luteum (CL) dysfunction, and stress hormones play a critical role in this process. However, the mechanism remains unclear. In this study, weaned sows were injected with synthetic adrenocorticotropic hormone (ACTH) for 7 d; whole‐genome bisulfite sequencing (WGBS) and RNA sequencing was used respectively to investigate the systematic association between ACTH administration and DNA methylation in CL and its relationship to gene expression. Results showed that ACTH treatment significantly increased the concentrations of Cortisol (P < 0.05). The genome‐wide DNA methylation maps of CL were provided, and the global analysis showed the difference between the 2 groups exists in the chromosomes and feature regions of the genome. A total of 88,559 DMRs were identified and the most DMR‐related genes were gathered in terms of metabolic biologic processes, and some DMR‐related genes were involved in cellular differentiation. Nine differentially expressed genes were screened out of coexpressed genes and 4 DMR‐associated genes that were also differentially expressed (P < 0.05). In summary, our study firstly provides insight into the regulation of ACTH administration on genomic DNA methylation and gene expression in CL. We revealed a remarkable alteration of DNA methylation in CL caused by ACTH treatment, and identified 4 DMR‐related genes that may be involved in the CL function under stress conditions.—Zhao, F., Wu, W., Wei, Q., Shen, M., Li, B., Jiang, Y., Liu, K., Liu, H. Exogenous adrenocorticotropic hormone affects genome‐wide DNA methylation and transcriptome of corpus luteum in sows. FASEB J. 33, 3264–3278 (2019). http://www.fasebj.org

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Section: Discussionmentioning

confidence: 99%

Exogenous adrenocorticotropic hormone affects genome‐wide DNA methylation and transcriptome of corpus luteum in sows

Zhao

Wei

et al. 2018

FASEB j.

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“…; Guelfi et al . ), and ACVR1C (or ALK7 ), which is expressed in the corpus luteum and influences its metabolism (Galvao et al . ).…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide detection of signatures of selection in Korean Hanwoo cattle

et al. 2014

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The Korean Hanwoo cattle have been intensively selected for production traits, especially high intramuscular fat content. It is believed that ancient crossings between different breeds contributed to forming the Hanwoo, but little is known about the genomic differences and similarities between other cattle breeds and the Hanwoo. In this work, cattle breeds were grouped by origin into four types and used for comparisons: the Europeans (represented by six breeds), zebu (Nelore), African taurine (N'Dama) and Hanwoo. All animals had genotypes for around 680 000 SNPs after quality control of genotypes. Average heterozygosity was lower in Nelore and N'Dama (0.22 and 0.21 respectively) than in Europeans (0.26-0.31, with Shorthorn as outlier at 0.24) and Hanwoo (0.29). Pairwise FST analyses demonstrated that Hanwoo are more related to European cattle than to Nelore, with N'Dama in an intermediate position. This finding was corroborated by principal components and unsupervised hierarchical clustering. Using genome-wide smoothed FST , 55 genomic regions potentially under positive selection in Hanwoo were identified. Among these, 29 were regions also detected in previous studies. Twenty-four regions were exclusive to Hanwoo, and a number of other regions were shared with one or two of the other groups. These regions overlap a number of genes that are related to immune, reproduction and fatty acid metabolism pathways. Further analyses are needed to better characterize the ancestry of the Hanwoo cattle and to define the genes responsible to the identified selection peaks.

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“…Some studies suggest that CRH, glucocorticoids, and ACTH affect ovarian function at the hypothalamus and the pituitary gland levels to decrease the synthesis and release of gonadotropin-releasing hormone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH), whereas others suggest that they act on the ovary to modulate steroidogenesis and/or oogenesis directly [18][19][20]. However, evidence regarding the direct effect of the HPA products on the oocyte is limited and conflicting.…”

Section: Introductionmentioning

confidence: 99%

Restraint Stress Impairs Oocyte Developmental Potential in Mice: Role of CRH-Induced Apoptosis of Ovarian Cells1

Liang

Wei

Cheng

et al. 2013

Biology of Reproduction

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This study examined the role of CRH-induced ovarian cell apoptosis in the restraint stress (RS)-induced impairment of oocyte competence. Oocyte percentages of apoptotic cumulus cells (CCs) did not differ between stressed and control mice before in vitro maturation (IVM) but became significantly higher in stressed mice after IVM without serum, growth factor, and hormone. The level of Bcl2 mRNA decreased significantly in mural granulosa cells (MGCs) and ovarian homogenates after RS. Whereas ovarian estradiol, testosterone, and IGF1 decreased, cortisol and progesterone increased significantly following RS. RS increased the level of CRH in serum, ovary, and oocyte while enhancing the expression of CRHR1 in CCs, MGCs, and thecal cells. RS down-regulated ovarian expression of glucocorticoid receptor and brain-derived neurotrophic factor. Furthermore, CRH supplementation to IVM medium impaired oocyte developmental potential while increasing apoptotic CCs, an effect that was completely overcome by addition of the CRHR1 antagonist antalarmin. Results suggest that RS impaired oocyte competence by increasing CRH but not glucocorticoids. Increased CRH initiated a latent apoptotic program in CCs and oocytes during their intraovarian development, which was executed later during IVM to impair oocyte competence. Thus, elevated CRH interacted with increased CRHR1 on thecal cells and MGCs, reducing the production of testosterone, estrogen, and IGF1 while increasing the level of progesterone. The imbalance between estrogen and progesterone and the decreased availability of growth factors triggered apoptosis of MGCs and facilitated CC expression of CRHR1, which interacted with the oocyte-derived CRH later during IVM to induce CC apoptosis and reduce oocyte competence.

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Direct actions of ACTH on ovarian function of pseudopregnant rabbits

Cited by 18 publications

References 48 publications

Exogenous adrenocorticotropic hormone affects genome‐wide DNA methylation and transcriptome of corpus luteum in sows

Exogenous adrenocorticotropic hormone affects genome‐wide DNA methylation and transcriptome of corpus luteum in sows

Genome‐wide detection of signatures of selection in Korean Hanwoo cattle

Restraint Stress Impairs Oocyte Developmental Potential in Mice: Role of CRH-Induced Apoptosis of Ovarian Cells1

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