Dipterocarpus tuberculatus Roxb. Ethanol Extract Has Anti-Inflammatory and Hepatoprotective Effects In Vitro and In Vivo by Targeting the IRAK1/AP-1 Pathway

Kim, Haeyeop; Yang, Woo Seok; Htwe, Khin Myo; Lee, Mi-Nam; Kim, Young Dong; Yoon, Ki Dong; Lee, Byoung-Hee; Lee, Sarah; Cho, Jae Youl

doi:10.3390/molecules26092529

Cited by 7 publications

(3 citation statements)

References 44 publications

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“…However, little is known about the exact effect of TNF-α on P-gp in the placenta. Previous studies have revealed that 16) and the influence of inflammatory factors, particularly TNF-α (12,17). In this study, we found that rTNF-α induced upregulation of P-gp expression and binding activity to the AP-1 consensus DNA binding sequence, while the effects were attenuated by pretreatment with inhibitors of AP-1 (SR11302) or P-gp (SP600125).…”

Section: Discussionmentioning

confidence: 52%

Inflammatory factor tumor necrosis factor-α (TNF-α) activates P-glycoprotein (P-gp) by phosphorylating c-Jun and thus promotes transportation in placental cells

Li¹,

Yan²,

Li³

et al. 2022

Transl Pediatr

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Background: P-glycoprotein (P-gp), encoded by the ABCB1 gene, actively pumps drugs and other xenobiotics from trophoblast cells back into the maternal circulation and thus acts as one of the most critical protectors of the fetus. The effect of tumor necrosis factor-α (TNF-α) on P-gp and molecule-transporting activity remains unknown. The goal of this study was to investigate the role of TNF-α in placental moleculetransporting activity and the underlies mechanisms.Methods: Cultured human placental choriocarcinoma cell lines, Bewo, JEG-3 and JAR, were used in this study.Cultured cells were incubated with 5, 10 and 20 ng/mL of recombinant TNF-α (rTNF-α) for 24 h, respectively, for follow-up experiments. The dimer form and expression of activator protein-1 (AP-1) family members were detected using Western blot (WB) and chromatin immunoprecipitation (ChIP). mRNA and protein expression of ABCB1 were detected using reverse transcriptional quantitative polymerase chain reaction (RT-qPCR) and WB, respectively. Double luciferase labeling was used to verify the concentration of digoxin. Electromobility shift assay (EMSA) and ChIP were used to identify the binding ability of c-Jun to ABCB1 gene promoter. Proliferation and apoptosis of Bewo cells were determined using flow cytometry. Digoxin concentration were determined using dual luciferase labeling method.Results: Administration of rTNF-α upregulated the expression of c-Jun but not JunB or JunD in a dosedependent manner and promoted the binding of c-Jun to the ABCB1 promoter region in Bewo cells. rTNF-α also increased the uptake of two P-gp-specific substrates, Rh123 and DiOC 2 (3), a function reversed by the addition of SP600125 and SR11302. We also found that rTNF-α increased the efflux ratio of digoxin, an outcome that was reversed, as expected, by inhibiting c-Jun and P-gp binding activities. Furthermore, we identified that rTNF-α tightly regulates the molecule-transporting activity of P-gp by promoting the phosphorylation of c-Jun.Conclusions: TNF-α activates P-gp to promote placental molecule-transporting activity by directly upregulating c-Jun expression and phosphorylation. These findings demonstrate the clinical significance of TNF-α in modulating the placental barrier, which plays an important role in protecting fetus against harmful drugs.

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Section: Discussionmentioning

confidence: 52%

Inflammatory factor tumor necrosis factor-α (TNF-α) activates P-glycoprotein (P-gp) by phosphorylating c-Jun and thus promotes transportation in placental cells

Li¹,

Yan²,

Li³

et al. 2022

Transl Pediatr

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“…This plant's heartwood contains several quinone analogs that are considered antioxidants. Several studies have reported that this plant reduces NF-mediated inflammation [44]. Jung et al (2022) coated Ti implants with MED, a natural plant extract.…”

Section: Dipterocarpus Tuberculatusmentioning

confidence: 99%

The role of current herbal extracts in bone regeneration through dental implants: in vitro/ in vivo/ clinical studies

et al. 2022

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The quality and quantity of bone at the interface of an implant system is a determining factor in the implant's stability. Alternative agents have been studied to augment implants and bone defects, including bone-conductive and bone-inducing agents. By modifying implant surface coatings on the nanoscale, one can enhance osseointegration by stimulating bone cell adhesion, bone matrix formation, and mineralization. Because alternative agents stimulate osteoblasts to mineralize and can control pectin structure, plant-derived silicone has been suggested as a potential candidate for surface nanocoatings on orthopedic and dental titanium implants. Inducing the differentiation of cells or accelerating bone regeneration is possible with the plant extract. Coating these extracts on implant devices can improve cell attachment, differentiation, and proliferation. This review article discusses the role of herbal materials in bone regeneration through dental implants.

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“…Of the 69 keruing species, twenty can produce oil with medicinal and fragrance properties (Boer and Ella 2001). Keruing oil contains several chemical compounds such as humulene, sesquiterpene alcohol (Aslam et al 2015), oleanilic acid (Senathilake et al 2017), and vaticaffinol (Chen et al 2017), which are useful as diuretic drugs, wound infections (Kim et al 2021), inflammatory symptoms (Yang 2013), gout (Chen et al 2017) and antifilariae (Senathilake et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Floristic composition and diversity at Keruing (Dipterocarpus spp.) habitat in Tangkahan, Gunung Leuser National Park, Indonesia

Susilowati

Rachmat²,

Elfiati

et al. 2021

Biodiversitas

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Abstract. Susilowati A, Rachmat HH, Elfiati D, Hidayat A, Hadi AN, Zaitunah A, Nainggolan D, Ginting IM. 2021. Floristic composition and diversity at Keruing (Dipterocarpus spp.) habitat in Tangkahan, Gunung Leuser National Park, Indonesia. Biodiversitas 22: 4448-4456. Dipterocarpus spp., or locally known as Keruing, is a wood-producing tree and has high economic value. Keruing also produces derivative products in oil and oleoresin, which are widely used as raw materials for medicines and perfumes. Keruing natural population has been severely declining caused by habitat alteration and also over exploitation. Therefore, information on the composition and diversity of the floristic community at their habitat is important as an initial step in designing conservation actions. This study aims to determine the floristic composition and diversity at the keruing habitat in the Tangkahan area, Gunung Leuser National Park (Gunung Leuser NP). A line transect has been used to establish a purposive sampling in particular forest areas where the population of the keruing is known to grow naturally. Four line transects, each with five nested plots, totaling 110 plots, were established to gather data at four growth stages. The results found 47 species at the seedling stage, 43 species at the sapling stage, 48 species at the pole stage and 65 species at the tree stage. There were three species of keruing in the research location, namely Dipterocarpus haseltii, Dipterocarpus costulatus and Dipterocarpus elongatus Korth with low IVI values ??at each stage. D. haseltii was only found at the seedling, pole and tree stage with IVI values ??of 0.32, 0.30, and 6.90, respectively. D. costulatus was only found at the tree level with an IVI value of 3.43, while D. elongatus found at the sapling and tree stage with an IVI of 0.30 and 3.42, respectively. The diversity index in the research location was in the medium category at the seedling, sapling, and pole levels with a value of 2.96, 2.86, and 2.92, respectively and was classified as high at the tree level with a value of 3.18. The evenness index (E') in all growth stages is high or evenly distributed with the value of E' > 0.75. The species diversity in research location classified into medium-high category although in each stage showing dynamic on species domination. Disturbed habitat due to illegal logging and land conversion caused a problem on keruing regeneration. Conservation efforts, including active monitoring keruing occurrence, ex-situ conservation, and artificial regeneration, are needed to avoid keruing from extinction.

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Dipterocarpus tuberculatus Roxb. Ethanol Extract Has Anti-Inflammatory and Hepatoprotective Effects In Vitro and In Vivo by Targeting the IRAK1/AP-1 Pathway

Cited by 7 publications

References 44 publications

Inflammatory factor tumor necrosis factor-α (TNF-α) activates P-glycoprotein (P-gp) by phosphorylating c-Jun and thus promotes transportation in placental cells

Inflammatory factor tumor necrosis factor-α (TNF-α) activates P-glycoprotein (P-gp) by phosphorylating c-Jun and thus promotes transportation in placental cells

The role of current herbal extracts in bone regeneration through dental implants: in vitro/ in vivo/ clinical studies

Floristic composition and diversity at Keruing (Dipterocarpus spp.) habitat in Tangkahan, Gunung Leuser National Park, Indonesia

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