Dipterocarpus tuberculatus ethanol extract strongly suppresses in vitro macrophage-mediated inflammatory responses and in vivo acute gastritis

Yang, Woo Seok; Lee, Byoung-Hee; Kim, Shi Hyoung; Kim, Han Gyung; Yi, Young-Su; Htwe, Khin Myo; Kim, Young Dong; Yoon, Ki Dong; Hong, Sungyoul; Lee, Woo-Shin; Cho, Jae Youl

doi:10.1016/j.jep.2013.01.033

Cited by 24 publications

(18 citation statements)

References 35 publications

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“…Plants of genus Dipterocarpus have been reported to have many bioactivities including antibacterial, antioxidant [7], cytotoxic [8], anti-inflammatory [9] and anti-filarial activities [10]. Extracts of the bark and leaves of D. turbinatus used in Ayurvedic and Unani medicines showed potent cytotoxic activity against breast cancer cell lines [11].…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of Dipterocarpus alatus

et al. 2019

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Dipterocarpus alatus (Dipterocarpaceae) is a medicinal plant whose use is well known for the treatment of genito-urinary diseases. However, there is no report of its cytotoxic potential. In this study, the chemical composition, antioxidant and cytotoxic activities of extracts of the leaves, bark, twigs and oleo-resin from D. alatus are investigated. Cytotoxicity was measured by the neutral red (NR) assay against HCT116, SKLU1, SK-MEL2, SiHa and U937 cancer cell lines and antioxidant capacity was evaluated by DPPH, ABTS radical scavenging, and ferric reducing antioxidant power (FRAP) assays. The chemical composition was analyzed by gas chromatography–mass spectrometry (GC-MS). Leaf, bark and twig extracts exhibited stronger antioxidant activity than oleo-resin, with bark extract showing the highest antioxidant activity and the highest total phenolic content. All samples showed more cytotoxic activity against the U937 cell line than HCT116, SKLU1, SK-MEL2 and SiHa cells with oleo-resin being more cytotoxic than melphalan against U937 cells. Chemical composition analysis of oleo-resin by GC-MS showed that the major components were sesquiterpenes, namely α-gurjunene (30.31%), (-)-isoledene (13.69%), alloaromadendrene (3.28%), β-caryophyllene (3.14%), γ-gurjunene (3.14%) and spathulenol (1.11%). The cytotoxic activity of oleo-resin can be attributed to the sesquiterpene content, whereas the cytotoxic and antioxidant activities of leaf, bark and twig extracts correlated to total phenolic content.

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Section: Introductionmentioning

confidence: 99%

Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of Dipterocarpus alatus

et al. 2019

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“…A study involving 95% ethanol extracts of Dipterocarpaceae shows anti‐inflammatory effect on LPS‐treated macrophages by regulating IκBα, which in turn regulates the nuclear translocation of NF‐κB . PDK1 is not suppressed in this study, but the phosphorylation of PDK1 is strongly suppressed as well as upstream kinases of IκBα (IKK and Akt) . LPS increases the production of lactate, TNF‐α, IL‐6, and NO in macrophages, while PDK1 knockdown will end such increase in a mild manner .…”

Section: Pdk1 Regulates the Inflammation Of Macrophagementioning

confidence: 52%

“…43 PDK1 is not suppressed in this study, but the phosphorylation of PDK1 is strongly suppressed as well as upstream kinases of IκBα (IKK and Akt). 47,48 LPS increases the production of lactate, TNF-α, IL-6, and NO in macrophages, while PDK1 knockdown will end such increase in a mild manner. 49 All these findings indicate that PDK1 signal plays a complex and important role in inflammation of macrophages.…”

Section: Pdk1 Regulates the Inflammation Of Macrophagementioning

confidence: 99%

Regulation mechanism of PDK1 on macrophage metabolism and function

Yang

Kong

Xia

et al. 2016

Cell Biochemistry & Function

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PDK1 is a member of the atypical glandular cell kinases family that regulates the activities of most atypical glandular cell kinases during different development stages and treatment of cancers. PDK1 is also a critical glucose metabolism enzyme regulating glucolysis or glucose oxidase in cells, and more research is needed to further understand the underlying mechanism. The research of PDK1 presented by recent studies focuses much on cancer treatment and has helped researchers gain much insight in this regard. Given the close relationship between inflammation and cancer, it is of great significance to discover the function of PDK1 and its regulating mechanism on special immune cells-macrophages. This review summarizes the recent findings regarding PDK1 in terms of regulating the function and metabolism of macrophage. The mechanism of PDK1 in regulating inflammatory secretion, migration, phagocytosis, and the energy metabolism of macrophage and a possible path to develop PDK1 related pharmaceutical products are discussed as well.

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“…HCl/EtOH-induced gastritis. Stomach inflammation was induced in mice using HCl/EtOH according to a published method (Yang et al, 2013b). Fasted ICR mice were orally treated with Gl-ME (20 and 200 mg/kg), resveratrol (5 and 50 mg/kg), rofecoxib (10 mg/kg), or ranitidine (40 mg/kg) twice a day for three days.…”

Section: Methodsmentioning

confidence: 99%

Anti-inflammatory Activities of Gouania leptostachya Methanol Extract and its Constituent Resveratrol

et al. 2014

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Gouania leptostachya DC. var. tonkinensis Pitard. Rhamnaceae is a traditional medicinal plant used in Thailand for treating various inflammatory symptoms. However, no systematic studies have been performed concerning the anti-inflammatory effects or molecular mechanisms of this plant. The immunopharmacological activities of a methanol extract from the leaves and twigs of G. leptostachya (Gl-ME) were elucidated based on the gastritis symptoms of mice treated with HCl/EtOH and the inflammatory responses, such as nitric oxide (NO) release and prostaglandin E2 (PGE2) production, from RAW264.7 cells and peritoneal macrophages. Moreover, inhibitory target molecules were also assessed. Gl-ME dose-dependently diminished the secretion of NO and PGE2 from LPS-stimulated RAW264.7 cells and peritoneal macrophages. The gastritis lesions of HCl/EtOH-treated mice were also attenuated after Gl-ME treatment. The extract (50 and 300 µg/mL) clearly reduced mRNA expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2, nuclear translocation of p65/nuclear factor (NF)-κB, phosphorylation of p65-activating upstream enzymes, such as protein kinase B (AKT), inhibitor of κBα kinase (IKK), and inhibitor of κB (IκBα), and the enzymatic activity of Src. By HPLC analysis, one of the major components in the extract was revealed as resveratrol with NO and Src inhibitory activities. Moreover, this compound suppressed NO production and HCl/EtOH-induced gastric symptoms. Therefore, these results suggest that Gl-ME might be useful as an herbal anti-inflammatory medicine through the inhibition of Src and NF-κB activation pathways. The efficacy data of G. leptostachya also implies that this plant could be further tested to see whether it can be developed as potential anti-inflammatory preparation.

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Dipterocarpus tuberculatus ethanol extract strongly suppresses in vitro macrophage-mediated inflammatory responses and in vivo acute gastritis

Cited by 24 publications

References 35 publications

Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of Dipterocarpus alatus

Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of Dipterocarpus alatus

Regulation mechanism of PDK1 on macrophage metabolism and function

Anti-inflammatory Activities of Gouania leptostachya Methanol Extract and its Constituent Resveratrol

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