Dipsticks for rapid detection of <i>Plasmodium</i> in vectoring <i>Anopheles</i> mosquitoes

Ryan, Jeffrey R.; Dav, K; Emmerich, Enzo; García, Luz; Yi, L; Coleman, Russell E.; Sattabongkot, Jetsumon; Dunton, Raymond F.; Chan, Adeline; Wirtz, Robert A.

doi:10.1046/j.1365-2915.2001.00296.x

Cited by 28 publications

(35 citation statements)

References 11 publications

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“…To determine which species of Anopheles was transmitting malaria in this region an immunological assay (VecTest TM Malaria) that identifies the presence or absence of specific peptide epitopes of circumsporozoite (CS) protein of Plasmodium falciparum and two strains of P. vivax (variants 210 and 247) was used (Ryan et al 2001). This test has been extensively evaluated and presents an overall 92% sensitivity and 98.1% specifi city as compared to standard CS enzyme-linked immunosorbent assay (ELISA) (Ryan et al 2002).…”

Section: Malaria Sporozoite Infectionmentioning

confidence: 99%

Mosquito Anthropophily: implications on malaria transmission in the northern Brazilian Amazon

Barros¹,

Honório²,

Arruda³

2010

Neotrop. entomol.

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-Seasonal variation of adults and larvae of anophelines was studied during 2003 and 2004 in Roraima State, located in the Northern region of Brazilian Amazon. Species diversity increased with distance of capture to human dwellings. Greater diversity was found in extradomiciliary collections than in peridomiciliary or intradomiciliary. A signifi cant association between Anopheles darlingi Root and An. albitarsis (s.l.) Arribálzaga (Diptera: Culicidae) breeding sites and the proximity to human dwellings was observed. Malaria Sporozoite Antigen Panel Assay (Vectest TM Malaria) indicated An. albitarsis s.l. as one of the local vectors in the studied area. In this study, an index to describe the anthropophilic behavior of each anopheline species is proposed.

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Section: Malaria Sporozoite Infectionmentioning

confidence: 99%

Mosquito Anthropophily: implications on malaria transmission in the northern Brazilian Amazon

Barros¹,

Honório²,

Arruda³

2010

Neotrop. entomol.

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“…The ELISA uses species-speciÞc monoclonal antibodies (Mabs) to detect circumsporozoite protein (CSP), epitopes that are amply expressed on the surface of live or preserved sporozoites (Howard 1986;Wirtz et al 1985Wirtz et al , 1987Wirtz et al , 1992Rosenberg et al 1989). Recent technological advances have led to the development of a simple to use, rapid detection test strip (dipstick), the malaria sporozoite antigen panel assay or VecTest (Medical Analysis Systems, Camarillo, CA), as a possible alternative to the standard ELISA method (Ryan et al 2001).…”

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confidence: 99%

Evaluation of a Dipstick Malaria Sporozoite Panel Assay for Detection of Naturally Infected Mosquitoes

Bangs¹,

Rusmiarto²,

Gionar³

et al. 2002

J Med Entomol

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The determination of the presence or absence of malaria sporozoites in wild-caught Anopheles mosquitoes remains an integral component to the understanding of the transmission dynamics in endemic areas. To improve that capability, there has been on-going development of a new device using dipstick immunochromatographic technology for simplifying the testing procedure and reducing the time required to obtain results. As part of a larger multi-center effort, we evaluated the sensitivity and specificity of a prototype malaria sporozoite antigen panel assay (Medical Analysis Systems, Camarillo, CA) against three human Plasmodium species/polymorphs. The wicking (dipstick) assay was compared against a standard parasite antigen capture enzyme-linked immunosorbent assay (ELISA) for the detection of human circumsporozoite protein (CSP) in wild-caught mosquitoes. Over 6,800 Anopheles mosquitoes, representing 20 species collected from malaria endemic areas of Indonesia were tested either individually or in pools of up to 10 mosquitoes each. From 1,442 pooled test strip assays and ELISA formats, nine mosquito pools were found reactive for P.falciparum, P. vivax 210, or P. vivax 247 CSP. There was complete concordance between test strip results and ELISA results. Sensitivity was 100% and given some minor problems with false positives or negatives, specificity (n = 488) was 97%. Most strips judged as false positive produced very weak signals compared with negative control blank strips and paired ELISA-negative samples. The dipstick test proved technically simpler to perform and interpret than the ELISA and results were obtained within 15 min of exposure to mosquito suspension. This qualitative assay appears an attractive alternative to the CSP ELISA for detection of sporozoites in fresh or dried mosquitoes.

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“…Immunoassay detection as well as immunohistochemical methods are needed for rapid and accurate detection at the onset of the disease. Quantum dots as labels of antibodies for fluorescent signal generation have become a viable alternative to enzyme and fluorescent dyes [7,8,[14][15][16][17][18][19][20][21][22][23][24]. In this project, we used two kinds of antibodies.…”

Section: Resultsmentioning

confidence: 99%

Breast Cancer Cell Imaging using Semiconductor Quantum Dots

Aguilar

et al. 2009

ECS Trans.

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Breast cancer is the second most common type of cancer, the fifth most common cause of cancer deaths in the US and the world, and the most common cause of cancer death in women. Knowing the accurate stage of the breast cancer is critical for patient survival. Sufficient treatment during the early stages of breast cancer can prevent the spread and transfer of the cancer cells to other parts of the body. But, the cancer cells need to be diagnosed at the early stage in order to start treatment. We report the development of early stage detection of breast cancer cells using quantum dots (QDs) as fluorescent signal generator for detection. The QDs based imaging of breast cancer cells involved two kinds of antibodies: the first was for labeling the cells and the second was for imaging the breast cancer cells. Anti-Her2/neu (1 0 Ab) was used for labeling the captured SK-BR3 cells. The second antibody against antiHer2/neu (2 0 Ab) that was conjugated to QDs (2 0 Ab~QDs) forms the complete assay, SK-BR3 + 1 0 Ab + 2 0 Ab~QDs, to generate the fluorescent cells for imaging. Fluorescent images of the complete assay for SK-BR3 cells were evaluated under a microscope with a UV light source. The preliminary results showed that the breast cancer cell SK-BR3 in the complete assay were successfully observed as fluorescent cells that had brighter signals compared with those labeled with organic dye using similar parameters and the same number of cells. Future directions will involve elimination of non-specific signals, establishment of dynamic range of concentrations detected as well as limits of detection, and multiplex detection of different types cells and/or biomarkers of cancer.

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Dipsticks for rapid detection of Plasmodium in vectoring Anopheles mosquitoes

Cited by 28 publications

References 11 publications

Mosquito Anthropophily: implications on malaria transmission in the northern Brazilian Amazon

Mosquito Anthropophily: implications on malaria transmission in the northern Brazilian Amazon

Evaluation of a Dipstick Malaria Sporozoite Panel Assay for Detection of Naturally Infected Mosquitoes

Breast Cancer Cell Imaging using Semiconductor Quantum Dots

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