Dipstick-type biosensor for visual detection of DNA with oligonucleotide-decorated colored polystyrene microspheres as reporters

Kalogianni, Despina P.; Litos, Ioannis; Christopoulos, Theodore K.; Ioannou, Penelope C.

doi:10.1016/j.bios.2008.08.056

Cited by 29 publications

(13 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Qualitative analysis primarily detects tumor-specific gene alterations ( 12 , 13 ), including gene mutations, promoter hypermethylation of tumor suppressor genes ( 14 ) and microsatellite alterations ( 15 ). Quantitative analyses primarily involve radioimmunity methods ( 16 ), polymerase chain reaction (PCR) amplification techniques ( 17 ), DNA nick translation labeling techniques ( 18 ) and DNA DipStick kits ( 19 ). These methods are associated with a number of issues, including radioactivity, environmental pollution problems, complex instructions and DNA loss during the process itself, which result in reduced sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of cell-free DNA in ovarian cancer

Shao

et al. 2015

Oncology Letters

View full text Add to dashboard Cite

The aim of the present study was to investigate the association between cell-free DNA (cf-DNA) levels and clinicopathological characteristics of patients with ovarian cancer using a branched DNA (bDNA) technique, and to determine the value of quantitative cf-DNA detection in assisting with the diagnosis of ovarian cancer. Serum specimens were collected from 36 patients with ovarian cancer on days 1, 3 and 7 following surgery, and additional serum samples were also collected from 22 benign ovarian tumor cases, and 19 healthy, non-cancerous ovaries. bDNA techniques were used to detect serum cf-DNA concentrations. All data were analyzed using SPSS version 18.0. The cf-DNA levels were significantly increased in the ovarian cancer group compared with those of the benign ovarian tumor group and healthy ovarian group (P<0.01). Furthermore, cf-DNA levels were significantly increased in stage III and IV ovarian cancer compared with those of stages I and II (P<0.01). In addition, cf-DNA levels were significantly increased on the first day post-surgery (P<0.01), and subsequently demonstrated a gradual decrease. In the ovarian cancer group, the area under the receiver operating characteristic curve of cf-DNA and the sensitivity were 0.917 and 88.9%, respectively, which was higher than those of cancer antigen 125 (0.724, 75%) and human epididymis protein 4 (0.743, 80.6%). There was a correlation between the levels of serum cf-DNA and the occurrence and development of ovarian cancer in the patients evaluated. bDNA techniques possessed higher sensitivity and specificity than other methods for the detection of serum cf-DNA in patients exhibiting ovarian cancer, and bDNA techniques are more useful for detecting cf-DNA than other factors. Thus, the present study demonstrated the potential value for the use of bDNA as an adjuvant diagnostic method for ovarian cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of cell-free DNA in ovarian cancer

Shao

et al. 2015

Oncology Letters

View full text Add to dashboard Cite

show abstract

“…Specific oligonucleotide sequences or immunologic substances (e.g., hapten-antibody and hapten-protein) that offer extremely strong affinity to capture DNA amplicons have been investigated as DNA probe candidates. More recently, progress on DNA biosensors has enabled visual detection of DNA by employing gold nanoparticles [ 19 – 22 ] and colored polystyrene beads [ 23 ] as signal generators. Quantitative data can be recorded by intensity readout or by additional coupling with an enzyme [ 20 ], fluorescent dye [ 24 , 25 ], or radioactive conjugates [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive PCR-Dipstick DNA Chromatography for Multiplex Analysis of the Oral Microbiota

Tian

Sato

Niwa

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

A complex of species has been associated with dental caries under the ecological hypothesis. This study aimed to develop a rapid, sensitive PCR-dipstick DNA chromatography assay that could be read by eye for multiplex and semiquantitative analysis of plaque bacteria. Parallel oligonucleotides were immobilized on a dipstick strip for multiplex analysis of target DNA sequences of the caries-associated bacteria, Streptococcus mutans, Streptococcus sobrinus, Scardovia wiggsiae, Actinomyces species, and Veillonella parvula. Streptavidin-coated blue-colored latex microspheres were to generate signal. Target DNA amplicons with an oligonucleotide-tagged terminus and a biotinylated terminus were coupled with latex beads through a streptavidin-biotin interaction and then hybridized with complementary oligonucleotides on the strip. The accumulation of captured latex beads on the test and control lines produced blue bands, enabling visual detection with the naked eye. The PCR-dipstick DNA chromatography detected quantities as low as 100 pg of DNA amplicons and demonstrated 10- to 1000-fold higher sensitivity than PCR-agarose gel electrophoresis, depending on the target bacterial species. Semiquantification of bacteria was performed by obtaining a series of chromatograms using serial 10-fold dilution of PCR-amplified DNA extracted from dental plaque samples. The assay time was less than 3 h. The semiquantification procedure revealed the relative amounts of each test species in dental plaque samples, indicating that this disposable device has great potential in analysis of microbial composition in the oral cavity and intestinal tract, as well as in point-of-care diagnosis of microbiota-associated diseases.

show abstract

“…We believe that the multianalyte DNA biosensor will find wide applications in small molecular diagnosis laboratories and in point-of-care testing (field testing). In a previous report from our group (Kalogianni et al, 2009), we have shown that colored polystyrene microspheres can also be used as reporters instead of gold nanoparticles for detection of PEXT products. Therefore, it can be realized that in the present biosensor antibiotin-coated colored microspheres could be used as an alternative reporter.…”

Section: Discussionmentioning

confidence: 98%

“…The biosensor described and evaluated in this work has a fundamentally different configuration from those reported recently by our group (Kalogianni et al, 2009;Konstantou et al, 2009). In the previous reports, the DNA target (PCR product or PEXT reaction product) was captured by streptavidin and antidigoxigenin antibody immobilized at the test zone(s) of the biosensor.…”

Section: Figmentioning

confidence: 95%