“…With this in mind, we designed two probes containing the pyruval ( 1 ) or benzoyl ( 2 ) group conjugated to the dye, 6-amino-1-phenalenone ( 3 ). Although the photophysical properties of 3 have not been thoroughly characterized,26 it was selected since it contains an exocyclic amine in conjugation with the pi system of the dye. We reasoned that conversion of the amine to an amide via ligation to a pyruval or benzyl moiety would cause a blue shift in the absorption spectrum, and a red-shift ( i.e.…”