Diploid-tetraploid relationship among old-world members of the fish family Cyprinidae

Ohno, Susumu; Muramoto, Jun-ichi; Christian, L. C.; Atkin, N. B.

doi:10.1007/bf00293307

Cited by 226 publications

(110 citation statements)

References 8 publications

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“…This might have resulted from polyploidization. It is well known that tetraploidization and genome duplication occurred in common carp and crucian carp with 100 chromosomes (Ohno et al 1967;Yu et al 1989). They have been demonstrated to be diploidized, but some loci have not been completely transformed from tetraploid to diploid.…”

Section: Discussionmentioning

confidence: 99%

Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio

Guo

Gui

2008

Aquacult Int

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Microsatellites have become the preferred molecular markers for strain selection and genetic breeding in fish. In this study a total of 105 microsatellites were isolated and identified in gibel carp (Carassius auratus gibelio) by microsatellite sequence searches in GenBank and other databases and by screening and sequencing of positive clones from the genomic library enriched for AG and GATA repeats. Moreover, nineteen microsatellites were randomly selected to design locus-specific primer pairs, and these were successfully used to identify and discriminate different cultured strains of gibel carp including strains A, D, L, and F. Three different types of microsatellite pattern were distinguished by the number and length of fragments amplified from the 19 primer pairs, and some microsatellite primer pairs were found to produce different microsatellite patterns among strains and strain-specific fragments. In addition, some duplicated alleles were also detected in two microsatellite patterns. Therefore, the current study provides direct molecular markers to discriminate among different cultured strains for selective breeding and aquaculture practice of gibel carp.

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Section: Discussionmentioning

confidence: 99%

Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio

Guo

Gui

2008

Aquacult Int

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“…The sterile triploids grow faster than their parental diploids, and, consequently, they are bred commercially in vast aquaculture facilities in the Yangtze River drainage (14). Although the initial research documented that rapid and extensive genomic changes follow tetraploidization (15)(16)(17)(18), many questions about allopolyploidization remain unanswered.…”

Section: Significancementioning

confidence: 99%

Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross

Liu

Luo

Chai

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

118

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Polyploidy is much rarer in animals than in plants but it is not known why. The outcome of combining two genomes in vertebrates remains unpredictable, especially because polyploidization seldom shows positive effects and more often results in lethal consequences because viable gametes fail to form during meiosis. Fortunately, the goldfish (maternal) × common carp (paternal) hybrids have reproduced successfully up to generation 22, and this hybrid lineage permits an investigation into the genomics of hybridization and tetraploidization. The first two generations of these hybrids are diploids, and subsequent generations are tetraploids. Liver transcriptomes from four generations and their progenitors reveal chimeric genes (>9%) and mutations of orthologous genes. Characterizations of 18 randomly chosen genes from genomic DNA and cDNA confirm the chimera. Some of the chimeric and differentially expressed genes relate to mutagenesis, repair, and cancer-related pathways in 2nF 1 . Erroneous DNA excision between homologous parental genes may drive the high percentage of chimeric genes, or even more potential mechanisms may result in this phenomenon. Meanwhile, diploid offspring show paternal-biased expression, yet tetraploids show maternal-biased expression. These discoveries reveal that fast and unstable changes are mainly deleterious at the level of transcriptomes although some offspring still survive their genomic abnormalities. In addition, the synthetic effect of genome shock might have resulted in greatly reduced viability of 2nF 2 hybrid offspring. The goldfish × common carp hybrids constitute an ideal system for unveiling the consequences of intergenomic interactions in hybrid vertebrate genomes and their fertility.allopolyploidization | chimeric genes | transcriptomes | sequence validation | vertebrate P olyploidization is much rarer in vertebrates than in plants, and the reasons for this difference remain a mystery (1-3). Traditional explanations include barriers to sex determination, physiological and developmental constraints (especially nuclearcytoplasmic interactions and related factors) (2, 3), and genome shock or dramatic genomic restructuring (2-4). One type of polyploidization, allopolyploidization, involves the genomes of two species. Hybridization, accompanied by polyploidization, triggers vast genetic and genomic imbalances, including abnormal quadrivalent chromosomal groups, dosage imbalances, a high rate of DNA mutations and combinations, and other non-Mendelian phenomena (5-7). The effects of these imbalances are usually deleterious and are rarely advantageous. Imbalances in many plant crops determine the fate of the allopolyploid offspring. Genomic changes immediately follow allopolyploidization. Various and SignificanceWhy is polyploidization rarer in animals than in plants? This question remains unanswered due to the absence of a suitable system in animals for studying instantaneous polyploidization and the crucial changes that immediately follow hybridization. RNA-seq analyses discover exte...

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“…To our knowledge, the extant triploid form of Carassius auratus complex is actually evolutionary hexaploid (Zhou and Gui, 2002;Zhu et al, 2006), and the diploid form is tetraploid in which diplodization has completed (Ohno et al, 1967). In comparison with other unisexual forms in vertebrates, the extant triploid form of Carassius auratus complex with more than 150 chromosomes has undergone more than one genome polyploidy event, and occurred on the basis of the previous tetraploidization and subsequent diploidization of the extant diploid form.…”

Section: Unusually High Incidence and Evolutionary Origin Of Triploidmentioning

confidence: 99%

“…Carassius auratus complex has been generally thought to consist of both diploid form and triploid form that cannot be discriminated morphologically from each other (Apalikova et al, 2011;Gui and Zhou, 2010;Jakovlić and Gui, 2011;Takada et al, 2010), even though tetraploidization has been demonstrated to occur in the diploid form with 100 chromosomes (Ohno et al, 1967), and evolutionary hexaploid has been also suggested for the triploid form with more than 150 chromosomes (Zhou and Gui, 2002;Zhu et al, 2006). According to the current taxonomic category, the triploid form, also commonly known as gibel carp, silver crucian carp or Prussian carp, has been recognized as a subspecies gibel carp Carassius auratus gibelio (Cherfas, 1981;Jiang et al, 1983) of Carassius auratus, including the domestic goldfish, or even a separate species Carassius gibelio (Kalous et al, 2007;Kalous and Martin, 2011;Rylková et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

High male incidence and evolutionary implications of triploid form in northeast Asia Carassius auratus complex

Jiang

Wang

Zhou

et al. 2013

Molecular Phylogenetics and Evolution

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Diploid-tetraploid relationship among old-world members of the fish family Cyprinidae

Cited by 226 publications

References 8 publications

Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio

Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio

Genomic incompatibilities in the diploid and tetraploid offspring of the goldfish × common carp cross

High male incidence and evolutionary implications of triploid form in northeast Asia Carassius auratus complex

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