Diploid Spermatozoa in Rabbit Semen and Their Experimental Separation from Haploid Spermatozoa

Beatty, R. A.; Fechheimer, N. S.

doi:10.1093/biolreprod/7.2.267

Cited by 32 publications

(23 citation statements)

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“…The finding of diploid spermatozoa only in very young males of KE strain confirms the statement made by Beatty & Fechheimer (1972) that both age and strain affect the incidence of such spermatozoa. In their study on rabbit sperm¬ atozoa, they found the highest proportion (1-5%) of such abnormalities in the youngest AD-strain males.…”

Section: Discussionsupporting

confidence: 86%

The Passage of Abnormal Spermatozoa Through the Uterotubal Junction of the Mouse

Krzanowska¹

1974

Reproduction

108

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Summary. Adult female mice of an outbred strain and of the inbred C57BL strain were naturally or artificially inseminated with spermatozoa from 6-to 14-week-old males belonging to two inbred strains, C57BL and KE. Several hours after insemination, the females were killed and examined for the percentage of spermatozoa with morphologically abnormal heads found in the uterus and in the oviducal flushings. According to the degree of abnormality, misshapen spermatozoa were divided into five classes. In young KE males, large spermatozoa were observed and designated as diploid. The proportion of abnormal forms in the uterus depended on the strain and on the age of the males, and was highest in the group inseminated with spermatozoa from young C57BL strain mice. The proportion of abnormal spermatozoa in the oviducal flushings was greatly reduced, although positively related to the percentage of abnormalities in the uterus. This applied particularly to slight abnormalities, their percentage being about three times higher in the uterus than in the oviduct. Spermatozoa with severe abnormalities were either not found in the oviduct or their proportion was reduced more than ten times.The uterotubal junction appears to function as a barrier against morphologically abnormal spermatozoa, especially against severely deformed ones, although its selective action is not absolutely effective.

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Section: Discussionsupporting

confidence: 86%

The Passage of Abnormal Spermatozoa Through the Uterotubal Junction of the Mouse

Krzanowska¹

1974

Reproduction

108

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“…The means and standard errors (from the unweighted percentages) are sufficient summaries of each column for the present purposes and show that although the percentage of dead diploid spermatozoa is almost three times that of haploids, it is substantially less than 100%, and many diploid rabbit spermatozoa must therefore be alive when ejaculated. The discrepancy between the present (higher) values and those of Beatty & Fechheimer (1972) is most probably due to the use of formol citrate as a preservative in the present study. Observations on spermatozoa with two tails (the incidence and degrees of fusion between the two tails being variable) showed that such spermatozoa were not very successful in traversing, or even penetrating, the cervix (Table 3c), most probably because many of this small population of doubletailed spermatozoa were found to be far less motile than most of those with single tails.…”

Section: Ejaculated Spermatozoacontrasting

confidence: 89%

“…The highest recorded natural incidence of diploid spermatozoa in rabbits (about 1-6%) was found in young (20-40-week-old) bucks of the AD strain of dark chinchilla rabbits maintained at the Edin¬ burgh Institute of Animal Genetics (Beatty & Fechheimer, 1972 (Dott & Foster, 1975) Immediately after collection, one drop of an ejaculate was diluted with 3 drops of formol citrate and used to make nigrosin-eosin smears (as described above). The remainder was divided into three equal parts and each diluted with 1 ml 0-85 % saline, 1 ml 0-93 BM medium (Beatty, 1964) or 1 ml Hank's BSS and incubated at 37°C in sealed glass vials.…”

Section: Methodsmentioning

confidence: 99%

“…Diploid spermatozoa were identified by their larger and more pear-shaped heads (see Beatty & Fechheimer, 1972 ;Carothers & Beatty, 1975). There was no variation in the identification of diploids whether scored in nigrosin-eosin, Casarett or phase-contrast preparations.…”

Section: Methodsmentioning

confidence: 99%

“…From observations on the mean head area and DNA content of ejaculated rabbit spermatozoa (Beatty & Fechheimer, 1972;Carothers & Beatty, 1975) it has been shown that individual haploid and diploid spermatozoa can be recognized with a high degree of confidence by simple visual appraisal of head size.…”

Section: Introductionmentioning

confidence: 99%

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The survival and transport to the site of fertilization of diploid rabbit spermatozoa

Mortimer

1977

Reproduction

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Summary. The possible involvement of diploid spermatozoa in the production of triploid zygotes in the rabbit has been investigated in terms of the success of diploids relative to haploids in reaching the site of fertilization, with special reference to any possible selectivity of sperm transport within the female tract. The observed large significant reduction in the incidence of diploid spermatozoa between the ejaculate and the oviducts would greatly reduce the chances of, but not necessarily prevent, a diploid spermatozoon fertilizing an egg. This reduction appears to be due to a combination of the reduced survival of diploids in the female tract and a selectivity of sperm transport dependent on sperm motility.

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Biological Roots of the Human Individual

Piattelli‐Palmarini¹

1973

Novartis Foundation Symposia

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Diploid Spermatozoa in Rabbit Semen and Their Experimental Separation from Haploid Spermatozoa

Cited by 32 publications

References 4 publications

The Passage of Abnormal Spermatozoa Through the Uterotubal Junction of the Mouse

The Passage of Abnormal Spermatozoa Through the Uterotubal Junction of the Mouse

The survival and transport to the site of fertilization of diploid rabbit spermatozoa

Biological Roots of the Human Individual

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