Diploid/Polyploid Syntenic Shuttle Mapping and Haplotype-Specific Chromosome Walking Toward a Rust Resistance Gene (Bru1) in Highly Polyploid Sugarcane (2n ∼ 12x ∼ 115)

Cunff, Loïc Le; Garsmeur, Olivier; Raboin, Louis Marie; Pauquet, Jérôme; Telismart, Hugues; Selvi, A.; Grivet, Laurent; Philippe, Romain; Begum, Dilara; Deu, Monique; Costet, Laurent; Wing, Rod A.; Glaszmann, Jean Christophe; D’Hont, Angélique

doi:10.1534/genetics.108.091355

Cited by 113 publications

(105 citation statements)

References 46 publications

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“…Therefore, the release of cultivars that are resistant to brown rust is very important and is the most efficient form of disease control. The genetic inheritance of brown rust in sugarcane has been broadly studied, and some researchers have claimed that this resistance trait is controlled by one or a few genes (Asnaghi et al, 2004;Costet et al, 2012;Daugrois et al, 1996;Garsmeur et al, 2011;Glynn et al, 2013;Hogarth et al, 1993;Le Cunff et al, 2008;Parco et al, 2014;Raboin et al, 2006;Racedo et al, 2013;Ramdoyal et al, 2000;Sordi et al, 1988). In contrast to that shown for brown rust, almost every trait that is related to sugarcane production exhibits quantitative variations.…”

Section: Mixed Modeling Of Yield Components and Brown Rust Resistancementioning

confidence: 99%

“…The severity of brown rust is assessed on a particular scale (Amorim et al, 1987;Tai et al, 1981), and the frequency of full-sib genotypes in each severity class is influenced by the genetic basis of the parents of the family. Several researchers have reported that brown rust resistance is controlled by one or a few genes (Asnaghi et al, 2004;Costet et al, 2012;Daugrois et al, 1996;Garsmeur et al, 2011;Glynn et al, 2013;Hogarth et al, 1993;Le Cunff et al, 2008;Parco et al, 2014;Raboin et al, 2006;Racedo et al, 2013;Ramdoyal et al, 2000;Sordi et al, 1988). Costet et al (2012) showed that resistance to brown rust in modern polyploid sugarcane cultivars essentially depends on the major gene Bru1.…”

Section: Probabilities Segregations and Heritability Of Resistance mentioning

confidence: 99%

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Mixed Modeling of Yield Components and Brown Rust Resistance in Sugarcane Families

et al. 2016

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Sugarcane (Saccharum spp.) is a complex autopolyploid with high potential for biomass production that can be converted into sugar and ethanol. Genetic improvement is extremely important to generate more productive and resistant cultivars. Populations of improved sugarcane are generally evaluated for several traits simultaneously and in multi-environment trials. In this study, we evaluated two full-sib families of sugarcane (SR1 and SR2) at two locations and 3 yr for stalk diameter, stalk height, stalk number, stalk weight, soluble solid content (Brix), sucrose content of cane, sucrose content of juice, fi ber, cane yield, sucrose yield, and resistance to brown rust (Puccinia melanocephala). Using a mixed model approach, we included appropriate variance-covariance (VCOV) structures for modeling heterogeneity and correlation of genetic eff ects and nongenetic residual eff ects. Th e genotypic correlations between traits were calculated across the adjusted means as the standard Pearson product-moment coeffi cient. Th rough the VCOV structures estimated for each trait, in general, the heritabilities ranged from 0.78 to 0.94. Additionally, we detected 17 and 12 signifi cant genotypic correlations between the evaluated traits for SR1 and SR2, respectively. Th e analysis of the severity data for brown rust revealed that 66 and 32% of the full-sib genotypes in SR1 and SR2, respectively, had at least 90% probability of being resistant. Abbreviations: AIC, Akaike information criterion; BIC, Bayesian information criterion; BLUP, best linear unbiased prediction; FIB, fi ber; GEI, genotype ´ environment interaction; GLMM, generalized linear mixed model; LMM, linear mixed model; METs, multi-environment trials; POL%C, sucrose content of cane in percentage; POL%J, sucrose content of juice in percentage; REML, restricted maximum likelihood; SD, stalk diameter; SH, stalk height; SN, stalk number; SR1, SP80-3280 ´ RB835486 full-sib family of sugarcane 1; SR2, SP81-3250 ´ RB925345 full-sib family of sugarcane 2; SW, stalk weight; TCH, tonnes of cane per hectare; TPH, tonnes of sucrose per hectare; VCOV, variance-covariance. core ideas• A linear mixed model is efficient in production data analysis of sugarcane.• In general, the broad-sense heritability of the traits were high, ranging from 0.78 to 0.94.• A generalized linear mixed model can be applied in brown rust analysis of sugarcane.• Multi-environment trials were applied to the genetic improvement of sugarcane.

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Section: Mixed Modeling Of Yield Components and Brown Rust Resistancementioning

confidence: 99%

Section: Probabilities Segregations and Heritability Of Resistance mentioning

confidence: 99%

Mixed Modeling of Yield Components and Brown Rust Resistance in Sugarcane Families

et al. 2016

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“…Therefore, the opportunity exists to utilize Bru1 in marker-assisted breeding and selection in order to improve brown rust resistance in sugarcane [29]. Bru1 has been used as a marker to identify if sugarcane genotypes are potentially resistant to brown rust [25,30,31]. Since 2009, this marker has also been utilized in the CP program to direct breeding strategies for brown rust resistance [29].…”

Section: Bru1 In Parental Clones and Rust Rating In Their Progenymentioning

confidence: 99%

Screening for Sugarcane Brown Rust in the First Clonal Stage of the Canal Point Sugarcane Breeding Program

Zhao

Davidson²,

Baltazar³

et al. 2015

Agronomy

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Sugarcane (Saccharum spp.) brown rust (caused by Puccinia melanocephala Syd. & P. Syd.) was first reported in the United States in 1978 and is still one of the great challenges for sugarcane production. A better understanding of sugarcane genotypic variation in response to brown rust will help optimize breeding and selection strategies for disease resistance. Brown rust ratings were scaled from non-infection (0) to severe infection (4) with intervals of 0.5 and routinely recorded for genotypes in the first clonal selection stage of the Canal Point sugarcane breeding program in Florida. Data were collected from 14,272 and 12,661 genotypes and replicated check cultivars in 2012 and 2013, respectively. Mean rust rating, % infection, and severity in each family and progeny of female parent were determined, and their coefficients of variation (CV) within and among families (females) were estimated. Considerable variation exists in rust ratings among families or females. The families and female parents with high susceptibility or resistance to brown rust were identified and ranked. The findings of this study can help scientists to evaluate sugarcane crosses and parents for brown rust disease, to use desirable parents for crossing, and to improve genetic resistance to brown rust in breeding programs. OPEN ACCESSAgronomy 2015, 5 342

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“…A base da herança genética de resistência a essa doença tem sido amplamente estudada e alguns autores defendem que a resistência é controlada por um ou poucos genes (18,25,26,27,28,29,30,31,32,33). Um gene de resistência, denominado Bru1, foi o primeiro caráter mendeliano descrito para o complexo genoma da cana-de-açúcar (18,26,30,32).…”

Section: Introductionunclassified

“…Estudos genômicos têm mostrado que Bru1 está incluído numa inserção de tamanho desconhecido (31,34). Esta inserção induz redução de recombinação, resultando em forte desequilíbrio de ligação (LD) na região Bru1 e um completo LD entre dois marcadores moleculares, R12H16 e 9O20-F4.…”

Section: Introductionunclassified

Validação de marcadores moleculares associados à resistência à ferrugem marrom em cana-de-açúcar

et al. 2017

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A ferrugem marrom causada pelo fungo Puccinia melanocephala é uma importante doença em cana-de-açúcar (Saccharum spp.) e está presente em praticamente todas as áreas de cultivo. Um gene de efeito maior, Bru1, foi descrito como fonte de resistência durável da cana-de-açúcar contra isolados de P. melanocephala. O objetivo deste estudo foi avaliar a eficiência de dois marcadores moleculares fortemente associados ao gene Bru1, R12H16 e 9O20-F4-RsaI, para predição da resistência genética à ferrugem marrom em variedades de cana-de-açúcar. ABSTRACTBrown rust caused by the fungus Puccinia melanocephala is an important disease affecting sugarcane (Saccharum spp.) and is present in almost all growing areas. A major effect gene, Bru1, was described as a durable resistance source in sugarcane against isolates of P. melanocephala. The aim of this study was to evaluate the efficiency of two molecular markers strongly associated with Bru1 gene, R12H16 and 9O20-F4-RsaI, for prediction of genetic resistance to brown rust in sugarcane varieties. Thus, this study included 14 RB varieties, developed by RIDESA (Interuniversity Network for the Development of the Sugarcane Industry), which were among the 10 most cultivated varieties in the central-south region of Brazil in the period of 1974-2015. To evaluate the disease in the field an experiment was conducted in randomized blocks with four replicates. The severity of Barreto, F.Z.; Balsalobre, T.W.A. Chapola, R.G.; Hoffmann, H.P.; Carneiro, M.S. Validation of molecular markers associated with brown rust resistance in sugarcane. Summa Phytopathologica, v.43, n.1, p.36-40, 2017. brown rust was quantified according to a diagrammatic scale ranging from 1 (most resistant) to 9 (most susceptible), and the obtained data were analyzed by using a generalized linear mixed model (GLMM). Concomitantly, total DNA was extracted from the 14 varieties, followed by amplification of the molecular markers, which were classified into haplotypes. Out of the total varieties, 12 had no symptoms of the disease and showed 99.35% probability of being resistant according to analysis via GLMM; among them, 91.66% were haplotype 1, i.e., had the presence of both markers associated with Bru1 gene. Therefore, we can conclude that the molecular markers R12H16 and 9O20-F4-RsaI were highly efficient in predicting resistant phenotypes and that Bru1 gene is probably the main source of sugarcane resistance to brown rust in commercial fields in Brazil.Keywords: Saccharum spp., breeding, Puccinia melanocephala, Bru1, disease resistance.A cana-de-açúcar (Saccharum spp.) está entre as mais importantes espécies cultivadas, principalmente para produção de açúcar e bioetanol (1). O Brasil é atualmente o país com a maior produção de cana-deaçúcar com mais de nove milhões de hectares cultivados e produtividade estimada em 76.909 kg ha -1 na safra 2015/16 (1, 2). A cana-de-açúcar é uma gramínea semi-perene com propagação vegetativa, sendo que os programas de melhoramento genético têm concentrado esforços para o desenvolviment...

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Diploid/Polyploid Syntenic Shuttle Mapping and Haplotype-Specific Chromosome Walking Toward a Rust Resistance Gene (Bru1) in Highly Polyploid Sugarcane (2n ∼ 12x ∼ 115)

Cited by 113 publications

References 46 publications

Mixed Modeling of Yield Components and Brown Rust Resistance in Sugarcane Families

Mixed Modeling of Yield Components and Brown Rust Resistance in Sugarcane Families

Screening for Sugarcane Brown Rust in the First Clonal Stage of the Canal Point Sugarcane Breeding Program

Validação de marcadores moleculares associados à resistência à ferrugem marrom em cana-de-açúcar

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