Diploid and hyperdiploid rat Schwann cell strains displaying negative autoregulation of growth in vitro and myelin sheath-formation in vivo

Haynes, Laurence W.; Rushton, James A.; Perrins, Matthew F.; Dyer, Jason K.; Jones, R. W.; Howell, R T

doi:10.1016/0165-0270(94)90120-1

Cited by 17 publications

(16 citation statements)

References 26 publications

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“…The SC line (SCL4.1/F7) was expanded in 75 cm 2 tissue culture flasks under proliferation conditions as described by the studies in which this cell line was established . Confluent monolayers were dissociated with Trypsin‐EDTA for passages and cellular encapsulation.…”

Section: Methodsmentioning

confidence: 99%

Tailoring 3D hydrogel systems for neuronal encapsulation in living electrodes

Aregueta‐Robles

Martens

Poole-Warren

et al. 2017

J Polym Sci B Polym Phys

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State‐of‐the‐art neurorprostheses rely on stiff metallic electrodes to communicate with neural tissues. It was envisioned that a soft, organic electrode coating embedded with functional neural cells will enhance electrode‐tissue integration. To enable such a device, it is necessary to produce a cell scaffold with mechanical properties matched to native neural tissue. A degradable poly(vinyl alcohol) (PVA) hydrogel was tailored to have a range of compressive moduli through variation in macromer composition and initiator amount. A regression model was used to predict the amount of initiator required for hydrogel polymerization with nominal macromer content ranging between 5 and 20 wt %. Hydrogels at 5 and 10 wt % were reliably formed but 15 wt % and above were not able to be fabricated due to the light attenuation properties of the initiator ruthenium at increased concentration. Compressive modulus of hydrogels decreased upon incorporation of biomolecules (sericin and gelatin), however, the bulk stiffness spanned the range required to match neural tissue properties (0.04–20kPa). Neuroglia cells, such as Schwann cells survived and grew within the scaffold. The significant finding of this work is that the PVA‐tyramine system can be tuned to provide a soft degradable scaffold for neural tissue regeneration while presenting bioactive molecules for cellular expansion. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2018, 56, 273–287

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Section: Methodsmentioning

confidence: 99%

Tailoring 3D hydrogel systems for neuronal encapsulation in living electrodes

Aregueta‐Robles

Martens

Poole-Warren

et al. 2017

J Polym Sci B Polym Phys

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“…This is due to the possible changes in cell behavior and associated phenotypic and genotypic alterations that may result from long-term exposure to proliferation medium29. Evidence has shown that SCs can be transformed and become growth factor-independent if culture is extended over 20 passages3031. Even though producing fresh primary cultures would be ideal for experimental procedures such as transplantation, long-term maintenance of cultures is often laborious, time-consuming, or impractical for certain applications.…”

Section: Resultsmentioning

confidence: 99%

A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves

Andersen

Srinivas

Piñero

et al. 2016

Sci Rep

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We herein developed a protocol for the rapid procurement of adult nerve-derived Schwann cells (SCs) that was optimized to implement an immediate enzymatic dissociation of fresh nerve tissue while maintaining high cell viability, improving yields and minimizing fibroblast and myelin contamination. This protocol introduces: (1) an efficient method for enzymatic cell release immediately after removal of the epineurium and extensive teasing of the nerve fibers; (2) an adaptable drop-plating method for selective cell attachment, removal of myelin debris, and expansion of the initial SC population in chemically defined medium; (3) a magnetic-activated cell sorting purification protocol for rapid and effective fibroblast elimination; and (4) an optional step of cryopreservation for the storage of the excess of cells. Highly proliferative SC cultures devoid of myelin and fibroblast growth were obtained within three days of nerve processing. Characterization of the initial, expanded, and cryopreserved cell products confirmed maintenance of SC identity, viability and growth rates throughout the process. Most importantly, SCs retained their sensitivity to mitogens and potential for differentiation even after cryopreservation. To conclude, this easy-to-implement and clinically relevant protocol allows for the preparation of expandable homogeneous SC cultures while minimizing time, manipulation of the cells, and exposure to culture variables.

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“…Catecholaminergic cells also appear in the prenatal period probably implied too in neurogenesis [18,25,26]. Very early in the rombencephalon area (E11-E12) serotonergic neurons are already presented, they will form later the mesencephalic raphe nuclei, also observed in some early hypothalamic nuclei [14].…”

Section: Discussionmentioning

confidence: 99%

Molecular signaling of 5-HT<sub>1A</sub> and presence of serotonergic cells in the fetal cerebral cortex

de¹,

Gutiérrez²,

Rodríguez³

2013

WJNS

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Early appearance of the serotonergic system in the fetal brain and the various effects of serotonin (5-HT) on brain morphogenesis, have given support to a neurotrophic role of serotonin. This function of serotonin is accomplished through a system of serotonin nerve terminals in the target regions that involves various 5-HT receptors. In visual, auditory and somatosensory cortex an early and intense serotonergic innervation is particularly important. The neuronal somata of these terminals are normally located in the mesencephalon and they have not been observed in the maturing cerebral cortex, neither in the adult brain. By using immunolabeling techniques, fluorescence and confocal microscopy, we observe the presence of both, 5-HT terminals and 5-HT cells in mesencephalon (Me, E17) and in the neopallium (Np, E13-E16) cocultures. Cells immunopositive to 5-HT and to tryptophan-5-hydroxilase are also observed in the Np on day 12 of culture. These results concerning the unexpected presence of serotonergic cells in the fetal cerebral cortex are interesting and may be of importance in corticogenesis. As it happens with other elements of the serotonergic system, the presence of these phenotypically serotonergic cells in the early cerebral cortex may be transitory and probably supporting cortex maturation processes. The molecular signaling path of the 5-HT 1A receptor has also been identified.

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Diploid and hyperdiploid rat Schwann cell strains displaying negative autoregulation of growth in vitro and myelin sheath-formation in vivo

Cited by 17 publications

References 26 publications

Tailoring 3D hydrogel systems for neuronal encapsulation in living electrodes

Tailoring 3D hydrogel systems for neuronal encapsulation in living electrodes

A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves

Molecular signaling of 5-HT<sub>1A</sub> and presence of serotonergic cells in the fetal cerebral cortex

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Diploid and hyperdiploid rat Schwann cell strains displaying negative autoregulation of growth in vitro and myelin sheath-formation in vivo

Cited by 17 publications

References 26 publications

Tailoring 3D hydrogel systems for neuronal encapsulation in living electrodes

Tailoring 3D hydrogel systems for neuronal encapsulation in living electrodes

A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves

Molecular signaling of 5-HT&lt;sub&gt;1A&lt;/sub&gt; and presence of serotonergic cells in the fetal cerebral cortex

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Molecular signaling of 5-HT<sub>1A</sub> and presence of serotonergic cells in the fetal cerebral cortex