Diphosphopyridine Nucleotidase in the Life Cycle of Neurospora Crassa

Zalokar, Marko; Cochrane, Vincent W.

doi:10.1002/j.1537-2197.1956.tb10471.x

Cited by 30 publications

(26 citation statements)

References 15 publications

(1 reference statement)

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“…To answer the initial question of how germination and mycelial differentiation occur in the presence of high concentrations of NADase (3,6,13), it was necessary to follow the levels of the enzyme through a complete asexual cycle (i.e., from conidia through maturation of mycelia, the formation of conidiophores, and the production of new conidia). Results obtained from samples of Neurosporn taken at nine intervals that encompassed the asexual cycle (Table 11) showed that the enzyme decreased rapidly during germination and early wild-type mycelial growth and that, although synthesized in older wild-type mycelia, it leached from the mycelia into the medium.…”

Section: Discussionmentioning

confidence: 99%

“…The asexual cycle begins with the conidial inoculum, and is followed by the outgrowth of the germ tube to form mature mycelium, the differentiation of the mycelium to form conidiophores, and the development of new conidia from these conidiophores (8, 9 ( (3,13).…”

Section: Nadase Determinations During the Asexual Cyclementioning

confidence: 99%

“…About 3 X 109 conidia were suspended in 6 ml of 0.6% cysteine HCI in 0.1 M KHC03 at pH 8.4 (13). This suspension was pipetted into a 20-1111 stainless steel disintegration capsule, which contained 2 g of alundum 90-mesh.…”

Section: Conidial and Mycelial Disintegrationmentioning

confidence: 99%

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Enzyme activities during the asexual cycle of Neurospora crassa. III. Nicotinamide adenosine diphosphate glycohydrolase

Gj¹

1969

Can. J. Microbiol.

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STINE, G. J. 1969. Enzyme activities during the asexual cycle of Neurospora crassa. 111. Nicotinamide adenosine diphosphate glycohydrolase. Can. J. Microbial. 15: 1249-1254. The level and specific activity of nicotinamide adenosine diphosphate glycohydrolase (NADase) were followed throughout the asexual cycle in Nelrrospora crassa. A large quantity of NADase associated with the conidia was water soluble. NADase activity rapidly disappeared from the growth medium during conidial germination and from early logarithmic mycelial growth. As the mycelia aged, the enzyme accumulated in the growth medium and in the aging aconidial conidiophores, regardless of their inability to produce conidia. Since the enzyme was synthesized as the culture aged, it appears that the conidiophores age quickly with respect to their production of NADase. Conidia produced from conidiophores of the wild-type culture contained about the same quantity of NADase as the inoculum conidia. The accumulation of NADase in the conidia of wild-type Neurospora is due to the normal differentiation of these conidia in the absence of a liquid environment. The enzyme becomes locked into the conidia being differcntiated from conidiophores that contain large amounts of the NADase activity.

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Section: Discussionmentioning

confidence: 99%

Section: Nadase Determinations During the Asexual Cyclementioning

confidence: 99%

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Enzyme activities during the asexual cycle of Neurospora crassa. III. Nicotinamide adenosine diphosphate glycohydrolase

Gj¹

1969

Can. J. Microbiol.

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“…A 1954 study reported the importance of light and oxygen in the synthesis of carotenoids of N. crassa (Zalokar, 1954). Furthermore, variations in respiratory enzymes such as NAD nucleotidase, β-galactosidase and β-glucosidase have been reported in conidia compared to the mycelia (Eberhart, 1961; Zalokar, 1959; Zalokar & Cochrane, 1956). The activity of the citric acid cycle was shown to decrease in conidia.…”

Section: Natural Variation and The Circadian Clockmentioning

confidence: 99%

Natural Variation of the Circadian Clock in Neurospora

Koritala

Lee

2017

Natural Variation and Clocks

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Most living organisms on earth experience daily and expected changes from the rotation of the earth. For an organism, the ability to predict and prepare for incoming stresses or resources is a very important skill for survival. This cellular process of measuring daily time of the day is collectively called the circadian clock. Because of its fundamental role in survival in nature, there is a great interest in studying the natural variation of the circadian clock. However, characterizing the genetic and molecular mechanisms underlying natural variation of circadian clocks remains a challenging task. In this chapter, we will summarize the progress in studying natural variation of the circadian clock in the successful eukaryotic model Neurospora, which led to discovering many design principles of the molecular mechanisms of the eukaryotic circadian clock. Despite the success of the system in revealing the molecular mechanisms of the circadian clock, Neurospora has not been utilized to extensively study natural variation. We will review the challenges that hindered the natural variation studies in Neurospora, and how they were overcome. We will also review the advantages of Neurospora for natural variation studies. Since Neurospora is the model fungal species for circadian study, it represents over 5 million species of fungi on earth. These fungi play important roles in ecosystems on earth, and as such Neurospora could serve as an important model for understanding the ecological role of natural variation in fungal circadian clocks.

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“…Various enzymes have been measured with respect to development in Neurospora crassa (10,11,12,13). Hoxvever, these investigations were concerned only with specific portions of the asexual cycle.…”

Section: Introductionmentioning

confidence: 99%

Enzyme Activities During the Asexual Cycle of Neurospora Crassa: I. Succinic Dehydrogenase

Stine¹

1967

Can. J. Microbiol.

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Separate extracts of I\ier~orospora crassa grown either in Vogel's medium N, medium N + glutamate, or medium N which had bee11 made deficient in zinc, were assayed for succinic dehydrogenase ancl soluble protein a t intervals throughout the asesunl cycle. Succinic dehydrogenase, although apparently unnecessary for either the formation of conidia or coniclial germination, does appear to be necessary for rnycelial growth and for the production of conidiophores. Differences in the amount of enzyme during morphologically distinct states of differentiation (i.e. production of the germ tube and production of the conidiophore) may serve as a n indicator of significant changes in the physiology of Nezlrospom a t these times during clevcloprnent. *.I h e level of succinic dehydrogenase in the conidiophore before its differentiation into conidia appears to influence the a~n o u~i t of this enzyme found in the conidia. This is in keeping with the idea that conditions prevailing in the cytoplasm before the formation of conidin may directly influence the constituents subseq~~ently found in the conidia which determine coniclial viability, rate of ger~nination, and growth yield.

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Diphosphopyridine Nucleotidase in the Life Cycle of Neurospora Crassa

Cited by 30 publications

References 15 publications

Enzyme activities during the asexual cycle of Neurospora crassa. III. Nicotinamide adenosine diphosphate glycohydrolase

Enzyme activities during the asexual cycle of Neurospora crassa. III. Nicotinamide adenosine diphosphate glycohydrolase

Natural Variation of the Circadian Clock in Neurospora

Enzyme Activities During the Asexual Cycle of Neurospora Crassa: I. Succinic Dehydrogenase

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