Diphenyl Diselenide Protects against Methylmercury-Induced Toxicity in <i>Saccharomyces cerevisiae</i> via the Yap1 Transcription Factor

Lovato, Fabricio Luís; Rocha, João Batista Teixeira da; Corte, Cristiane Lenz Dalla

doi:10.1021/acs.chemrestox.6b00449

Cited by 15 publications

(2 citation statements)

References 77 publications

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“…Since MeHg + or VCH increased GST activity, we expected to find an increase in the expression of Nrf2 and Keap1. Indeed, literature data have indicated an essential role for this transcription factor in the toxicity of MeHg + [106–108]. However, their expression did not differ from control flies.…”

Section: Discussionmentioning

confidence: 97%

Simultaneous exposure to vinylcyclohexene and methylmercury in Drosophila melanogaster: biochemical and molecular analyses

Piccoli

Segatto

Oliveira

et al. 2019

BMC Pharmacol Toxicol

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BackgroundExposure to vinylcyclohexene (VCH) and methylmercury (MeHg+) can induce oxidative stress and gene modulation. Several studies have been evaluating the effects of VCH and MeHg+, but little is known about interactive effects between them. This work aimed to assess the exposure and co-exposure effects of MeHg+ and VCH on oxidative stress and gene modulation in Drosophila melanogaster.MethodsReactive species production, glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities were evaluated after exposure and co-exposure to VCH (1 mM) and MeHg+ (0.2 mM) for one or three days in the head and body (thorax and abdomen) of flies. The expression of genes related to redox state and inflammatory response was evaluated after exposure and co-exposure to VCH and MeHg+ for three days.ResultsSurvival decreased only in flies co-exposed to VCH and MeHg+ for three days. All treatments increased total reactive species production after one day of exposure. However, no significant changes were observed in the head after three days of exposure. One day of exposure to VCH caused an increase in the head GST activity, whereas MeHg+ induced an increase after three days of exposure. Regarding the body, all treatments increased GST activity after one day of exposure, but only the flies exposed to MeHg+ presented an increase in GST activity after three days of exposure. Treatments did not alter AChE activity in the head. As for gene expression, there was a significant increase in the Relish transcription factor gene in the flies’ body, but Nrf2, Keap1, Jafrac1, TrxR1, and NF-κβ were not altered.ConclusionThe results suggest that exposure to VCH and MeHg+ induce oxidative stress and activation of an inflammatory response in fruit flies.

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Section: Discussionmentioning

confidence: 97%

Simultaneous exposure to vinylcyclohexene and methylmercury in Drosophila melanogaster: biochemical and molecular analyses

Piccoli

Segatto

Oliveira

et al. 2019

BMC Pharmacol Toxicol

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“…11). 25,306,307 The formation of R-Se-Hg-R adducts such as PhSeHgCH 3 was accompanied by a decrease in the liver, kidney, and brain Hg burden in mice, but not in rats. 308 In contrast to mice, the co-treatment with diphenyl diselenide and methylmercury caused an increase in the deposition of Hg in the brain and liver of rats, 308 suggesting that the formation of the complex PhSeHgCH 3 is followed by its rupture to insoluble mercury selenide (HgSe) (Fig.…”

Section: Potential Molecular Mechanisms Involved In the Pharmacologic...mentioning

confidence: 99%

Organic selenocompounds: are they the panacea for human illnesses?

Rocha

Nogara²,

Pereira³

et al. 2023

New J. Chem.

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Transcriptomic analysis of formic acid stress response in Saccharomyces cerevisiae

Zeng

Huang

Feng

et al. 2022

World J Microbiol Biotechnol

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Formic acid is a representative small molecule acid cell inhibitor in lignocellulosic hydrolysate, which can inhibit the growth of yeast cells in the process of alcohol fermentation. However, the mechanism of formic acid cytotoxicity remains largely unknown. This study aimed to study the cytotoxicity of formic acid stress to Saccharomyces cerevisiae. We evaluated the effects of formic acid on growth metabolism and cell morphology of yeast cells, and comprehensively and systematically analyzed the molecular mechanism of formic acid stress tolerance through transcriptome technology. The results showed that when the concentration of formic acid was 1.8 g/L, the growth of yeast cells was signi cantly inhibited, the cell surface was wrinkled, and the adhesion between cells was observed, and the cell wall and cell membrane of yeast were destroyed by changing the structure of proteins and carbohydrates, resulting in cell damage. Transcriptome sequencing results showed that formic acid stress inhibited protein biosynthesis, induced oxidative stress, resulted in autophagy, impaired intracellular ATP production and increased consumption, and then impaired normal physiological and metabolic functions of cells. Yeast cells provide su cient ATP by accelerating glucose metabolism, enhancing electron transport and ATP synthesis more energy to resist formic acid stress, and reduce the expression of genes related to energy metabolism such as intracellular amino acids to achieve an energy-saving strategy, In addition, it can also induce sexual reproduction and spore formation to improve cell tolerance to formic acid. This study initially revealed the molecular response mechanism of S. cerevisiae under formic acid stress, and provided a scienti c basis for further research on methods to improve the tolerance of cell inhibitors in lignocellulose hydrolysate.

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Diphenyl Diselenide Protects against Methylmercury-Induced Toxicity in Saccharomyces cerevisiae via the Yap1 Transcription Factor

Cited by 15 publications

References 77 publications

Simultaneous exposure to vinylcyclohexene and methylmercury in Drosophila melanogaster: biochemical and molecular analyses

Simultaneous exposure to vinylcyclohexene and methylmercury in Drosophila melanogaster: biochemical and molecular analyses

Organic selenocompounds: are they the panacea for human illnesses?

Transcriptomic analysis of formic acid stress response in Saccharomyces cerevisiae

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