Diphasic Action of Thyroxine Upon Succinic Dehydrogenase of Kidney Cortex, Heart Muscle, Adrenal Cortex, and Thyroid of Dog in Vitro

Sugisawa, Yuhsuke

doi:10.1507/endocrj1954.3.186

Cited by 4 publications

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“…In accordance with previous reports by Sugisawa (6,7), that thyroxine action is diphasic upon succinic dehydrogenase of mouse and dog tissues, the present experiment, carried out under experimental conditions similar to those adopted by the author cited above, showed that T3, TA4, TA3, TP4 and TP3 also affect the same enzyme of mouse heart in a diphasic way.…”

Section: Commentssupporting

confidence: 92%

Effect of Thyroid Hormones and Their Metabolic Products Upon Succinic Dehydrogenase Activity of Mouse Heart Muscle in Vitro

Yamamoto

Ishikawa

1959

Jpn.J.Physiol

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As to the effect of thyroxine upon succinic dehydrogenase activity, some observations have been made in this laboratory employing an improved Thunberg technique. Thyroxine was learned to be effective in a diphasic way in the sense that the hormone in its low concentration range activated succinic dehydrogenase, while inhibitory effect resulted at its relatively high concentrations, when homogenates of mouse and dog tissues were used as the enzyme sources (6, 7). Further detailed work was done on the mode of action of thyroxine upon the same enzyme of rat liver and heart muscle under various experimental conditions, in which concentrations of homogenate and substrate, succinate, were changed (11). Following up these observations, experiments were extended in order to test whether triiodothyronine, and the acetic and propionic acid analogues of thyroxine and triiodothyronine show a similar effect upon succinic dehydrogenase of heart muscle homogenate of mouse . MATERIALS AND METHODSAdult male mice of ddN strain, weighing approximately 20g. each, were employed.Animals were sacrificed by decapitation and heart muscle was excised, weighed on a torsion balance, homogenized with 500 volumes of distilled water using the whole-glass homogenizer, and the homogenate obtained was used for enzyme source.All these procedures were carried out at room temperature.The activity of succinic dehydrogenase was measured employing Thunberg tubes after Yamamoto and Sugisawa (11). The composition of the reaction mixture was as follows:In the main tube:1) 0.1M K2HPO4-KH2PO4 buffer (PH 7.4) 1.3 ml., 2) 0.2% homogenate 0.6ml., 3) hormone solutions of various Received for publication October 24, 1958.

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confidence: 92%