Dipeptidyl Peptidase IV (DPP-IV) Inhibitory Activity of Ultrafiltration and Gel Filtration Fraction of Gelatin Hydrolyaste Derived from Bone of Fish for Antidiabetes

Abstract

Bioactive peptides have been investigated largely for many biofunctional properties. The aim of this research was to determine the inhibitory activity of ultrafiltration (UF) and gel filtration (GF) fractions of gelatin derived from bone of Pangasius catfish against dipeptidyl peptidase IV (DPP-IV). Previous studies have shown that gelatin from skins of salmon, hake, halibut, milkfish, tilapia and bone of pangasius catfish have the activity in DPP-IV inhibition. While, as inhibitor, most of previous and recent studies shown that separation and fractination of gelatin hydrolysate increase their activity. This research was conducted in three stages including gelatin hydrolysates fractination by ultrafiltration (UF), UF fraction loaded into gel filtration (GF) and DPP-IV inhibition measurement. The fish bone gelatin was hydrolyzed using flavourzyme at three enzyme/substrate ratios (E/S of 3%, 6% and 9%) with incubation times 4, 6, and 8 h. Then, the hydrolysates fractioned by ultrafiltration with 3 kDa cutoff membrane continued with Superfine G-25 sephadex column (65 cm x 3 cm, flow rate 1 mL/min). The result shown that both group of fractions i.e UF and GF have inhibitory activity regarding their capacity to inhibit DPP-IV. The UF fraction >3 kDa derived from 9% (E/S) ratio for hdyrolysis were superb as DPP-IV inhibitor than other fractions, the highest one also has bioactivity higher than other previous fish gelatin fractions.

Valorization of fish byproducts: Sources to end‐product applications of bioactive protein hydrolysate

“…Moreover, TPH also showed a higher increase in the intracellular cAMP release as well as insulin secretion through the protein kinase A mechanism (Harnedy et al, 2018). Gelatin hydrolysate prepared from the bone of pangasius catfish using Flavourzyme was fractionated by ultrafiltration (3 kDa cutoff) membrane followed by gel filtration (Atma et al, 2019). Both fractions had DPP‐IV inhibitory activity, while UF fraction >3 kDa showed higher inhibitory activity than other fractions.…”

“…Recently, studies have shown that fish PH (FPH) derived from various parts of fish exhibits numerous bioactivities including antioxidant, antimicrobial, angiotensin-I converting enzyme (ACE) inhibition, calcium (Ca)-binding ability, antidiabetic, immunomodulatory, wound healing capacity, anticoagulant, anticancer, antifatigue, anti-aging, and neuroprotective effect (Altınelataman et al, 2019;Atma et al, 2019;Benjakul et al, 2018;Jiali Chen et al, 2020;Da Rocha et al, 2018;Hu et al, 2020;Kumar et al, 2019; J. K. Lee et al, 2019;Park & Jo, 2019). In this comprehensive review, sources, production, and purification of FPH or their derived peptides have been outlined.…”

Enrichment and Quantitation of Dipeptidyl Peptidase IV Inhibitory Peptides in Quinoa upon Systematic Malting

“…In previous studies, this process has been performed with different enzymes like alcalase, 19,20 trypsin, 21,22 or flavourzyme. 19,20,23 In some studies, the inhibitory effect of hydrolysates produced by a combination of multiple enzymes was investigated. 15,19,24 Another factor affecting the DPP-IV inhibitory activity in vivo is the further hydrolysis of peptides during gastrointestinal digestion, which can be simulated in vitro through a digest with pepsin, trypsin, and chymotrypsin.…”

“…In general, DPP-IV inhibitory peptides are released from proteins during hydrolysis. In previous studies, this process has been performed with different enzymes like alcalase, , trypsin, , or flavourzyme. ,, In some studies, the inhibitory effect of hydrolysates produced by a combination of multiple enzymes was investigated. ,, Another factor affecting the DPP-IV inhibitory activity in vivo is the further hydrolysis of peptides during gastrointestinal digestion, which can be simulated in vitro through a digest with pepsin, trypsin, and chymotrypsin. Although this process might be necessary for predicting how the food hydrolysate is taken up in the body and if it retains its inhibitory effect, it has only been performed in a limited number of studies. ,− …”