Dipeptide Phe-Cys derived from in silico thermolysin-hydrolysed RuBisCO large subunit suppresses oxidative stress in cultured human hepatocytes

Je, Jae‐Young; Cho, Young-Sook; Gong, Min; Udenigwe, Chibuike C.

doi:10.1016/j.foodchem.2014.09.022

Cited by 38 publications

(31 citation statements)

References 14 publications

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“…Intracellular lipid hydroperoxide content was determined using the fluorescence probe, Diphenyl-1-pyrenylphosphine (DPPP) (Je et al, 2015). Chang liver cells were seeded into 96-well black microplates at a concentration of 4 × 10 5 cells/mL.…”

Section: Lipid Peroxidation Inhibitory Assaymentioning

confidence: 99%

“…Cellular GSH content was determined using monobromobimane (mBBr) as a thiol staining reagent (Je et al, 2015). Cheng liver cells were seeded in black 96-well microplates at 4 × 10 5 cells/mL.…”

Section: Determination Of Intracellular Glutathione Contentmentioning

confidence: 99%

“…Superoxide dismutase (SOD) inactivates the superoxide radical by converting it to H2O2 and oxygen. H2O2 is further dissociated to oxygen and water by the action of catalase (Je et al, 2015). Glutathione peroxidase catalyses the reduction of hydroperoxides by glutathione.…”

Section: Antioxidant Enzyme Activity In Chang Liver Cellsmentioning

confidence: 99%

“…Oxidative stress occurs when the oxidative damage overwhelms the antioxidant defence mechanisms (Circu & Aw, 2010). Therefore, in an oxidative stress condition, the exogenous antioxidant support is required to either protect cellular macromolecules from oxidative attack or restore indigenous antioxidant defence molecules (Je, Cho, Gong, & Udenigwe, 2015). Since oxidative stress is strongly correlated with the neurodegenerative diseases, diabetes and inflammatory disorders (Szeto, 2008), extensive research has explored the capacity of exogenous antioxidant compounds to boost the defence mechanisms of living cells.…”

Section: Introductionmentioning

confidence: 99%

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Specifically designed peptide structures effectively suppressed oxidative reactions in chemical and cellular systems

Bamdad

Ahmed

Chen

2015

Journal of Functional Foods

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Section: Lipid Peroxidation Inhibitory Assaymentioning

confidence: 99%

Section: Determination Of Intracellular Glutathione Contentmentioning

confidence: 99%

Section: Antioxidant Enzyme Activity In Chang Liver Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Specifically designed peptide structures effectively suppressed oxidative reactions in chemical and cellular systems

Bamdad

Ahmed

Chen

2015

Journal of Functional Foods

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“…8 By simulating protein digestion and screening bioactive peptide from existing databases such as PepBank and BIOPEP, 9 in silico 'experiments' or digestion protocol could be conducted to evaluate various food source proteins for their potential to generate bioactive peptides. 10,11 This in silico digestion protocol has been used to investigate the potential for producing bioactive peptides from highly abundant, tissue-specific terrestrial food source proteins. [11][12][13] As for unicellular microorganisms, the abundance of cellular proteins is largely unknown and highly dynamic, depending on the cultivation conditions (availability of nutrition, light, temperature status, etc.).…”

Section: Introductionmentioning

confidence: 99%

Omics‐prediction of bioactive peptides from the edible cyanobacterium Arthrospira platensis proteome

Han

Zhang

et al. 2017

J Sci Food Agric

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BACKGROUND Bioinformatics approaches are widely used to evaluate the prospects of novel protein sources in bioactive peptide research. Edible cyanobacteria are considered as potential protein precursors. However, the abundance of unicellular cyanobacterial proteins is largely unknown and highly dynamic according to the cultivation conditions, which need to be considered in this research field. The objective of this work was to evaluate the protein abundance of Arthrospira platensis, as well as to map the bioactive peptide sequences from the high‐abundance proteins of the A. platensis proteome. RESULTS The high‐abundance proteins of the A. platensis proteome were identified with a high‐performance liquid chromatography–tandem mass spectrometry‐based method. A total of 593 proteins were detected and quantified. The occurrence frequency of the bioactive peptides in A. platensis proteome was calculated according to the amino acid sequences via the bioinformatics approaches. Further in silico digested by trypsin, pepsin and chymotrypsin, these proteins liberated 78, 99, and 96 bioactive peptides, respectively. In each case, angiotensin‐converting enzyme inhibitors and dipeptidyl peptidase IV inhibitors were enriched. CONCLUSION This work will help rationally design the protocols for cyanobacterial cultivation, protein pre‐treatment and peptide separation, and further produce more peptides with specific functions. © 2017 Society of Chemical Industry

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Study of the Antioxidant, Antimicrobial, and Wound Healing Properties of Raw Hydrolyzed Extract from Nile Tilapia Skin (Oreochromis niloticus)

Tozetto,

Santos Rocha,

Assis de Andrade

et al. 2023

Chemistry & Biodiversity

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Oreochromis niloticus (Nile tilapia) skin is a by‐product of Brazilian fish farming, rich in collagen. The present study aims to evaluate the wound healing, antioxidant, and antimicrobial potential of the raw hydrolyzed extract of Nile tilapia skin, as well as the identification of the main compounds. The in vitro activity was performed using antioxidant, antimicrobial and scratch wound healing assays. An in vivo experiment was performed to evaluate the wound healing potential. On days 1, 7, 14 and 21, the lesions were photographed to assess wound retraction and on the 7th, 14th and 21st days the skins were removed for histological evaluation and the blood of the animals was collected for glutamic oxaloacetic transaminase and glutamic pyruvic transaminase determination. The chemical study was carried out through liquid chromatography‐tandem mass spectrometry and de novo sequencing of peptides. The in vitro assays showed a reduction of the gap area in 24 h, dose‐dependent antimicrobial activity for both bacteria, and antioxidant activity. The chemical analysis highlighted the presence of active biopeptides. The histological evaluation showed that the raw hydrolyzed extract of Nile tilapia skin has a healing potential, and does not present toxicological effects; therefore, is promising for the treatment of wounds.

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Dipeptide Phe-Cys derived from in silico thermolysin-hydrolysed RuBisCO large subunit suppresses oxidative stress in cultured human hepatocytes

Cited by 38 publications

References 14 publications

Specifically designed peptide structures effectively suppressed oxidative reactions in chemical and cellular systems

Specifically designed peptide structures effectively suppressed oxidative reactions in chemical and cellular systems

Omics‐prediction of bioactive peptides from the edible cyanobacterium Arthrospira platensis proteome

Study of the Antioxidant, Antimicrobial, and Wound Healing Properties of Raw Hydrolyzed Extract from Nile Tilapia Skin (Oreochromis niloticus)

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