Dip-slide: an aid to quantitative urine culture in general practice.

Guttmann, Dennis; Naylor, G. R. E.

doi:10.1136/bmj.3.5561.343

Cited by 100 publications

(34 citation statements)

References 7 publications

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“…This was found to inhibit the growth of moulds on medium deliberately exposed to airborne contaminants, and such a concentration did not interfere with the growth of bacteria, including nine strains of coagulase negative staphylococci, isolated from pyogenic infections of the urinary tract, the number and size of the colonies being unchanged. We have found that the addition of cycloheximide to nutrient agar does not alter the relationship between dip-slide colony counts and pour plate viable counts (Guttmann & Naylor, 1967).…”

Section: Preparation Of Dip-slidesmentioning

confidence: 79%

“…We have compared dip-slide colony counts with viable bacterial counts by the pour plate method on 385 samples of urine from patients attending a urinary infection clinic (Guttmann & Naylor, 1967). We found that fewer than 40 colonies on the dip-slide always corresponds with a pour plate viable count of less than 104 organisms per ml., and that viable counts greater than 105 organisms per ml.…”

Section: Resultsmentioning

confidence: 96%

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The Dip-Slide: a modified dip-inoculum transport medium for the laboratory diagnosis of infections of the urinary tract

Naylor¹,

Guttmann

1967

J. Hyg.

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The dip-slide, consisting of a glass microscope slide coated with nutrient medium and inoculated by dipping in freshly voided urine, provides a simple measure of the bacterial concentration in fresh urine. This is a useful supplement to the usual microscopy and culture of urine in the diagnosis of urinary tract infections in general practice, when specimens cannot be delivered to a laboratory within several hours of collection, particularly when urine specimens have to travel by post. Dip-slides are also useful as the sole test in screening for symptomless bacteriuria.

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Section: Preparation Of Dip-slidesmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 96%

The Dip-Slide: a modified dip-inoculum transport medium for the laboratory diagnosis of infections of the urinary tract

Naylor¹,

Guttmann

1967

J. Hyg.

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“…Darüber hinaus müssen die Reaktionszeiten der einzelnen Felder bekannt sein und berücksichtigt werden (Leukozytenreaktion frühestens nach 2 min ablesen!). Naylor[17] zurückgeht. Dabei wird ein mit Agar versehener Träger (z.…”

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Rezidivierende Harnweginfektion der Frau — Diagnostik

Bruns

Piechota

Schneede

2006

Urologe

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The basis for the diagnostic work-up of recurrent cystitis is formed by taking a precise medical history against the background of knowledge of the pathogenesis of urinary tract infections. The anamnesis should also focus on factors that influence the natural flora (sexual intercourse, hygiene) but additionally include preceding antibiotic treatment and diseases that affect the immune status (diabetes mellitus). Urinalysis is the principal examination among the laboratory diagnostic procedures. The diagnosis is promptly confirmed by immediate analysis of a clean catch midstream urine sample using a counting chamber or a test strip. As a matter of principle, microbiological diagnosis always ensues. Extended diagnostic work-up (urological staging) is aimed at detecting functional and anatomic abnormalities. While these factors only play a subordinate role during the premenopausal phase, they gain in importance during the postmenopausal phase. A key role is also attributed to local estrogen deficiency.

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“…2 4 Dipslide cultures have been found useful for the diagnosis and follow-up of urinary tract infections from clean-voided urine specimens.35-8 However, low bacterial counts have not always been reliably detected on dipslides, which of course, is insignificant for clean-voided urine. 6 Therefore, we decided to investigate the occurrence of low bacterial counts in SPAs of infants and children, and to compare findings on dipslides and plates inoculated at the bedside with those of aerobic and anaerobic cultures from SPA samples taken to the laboratory in a transport medium.…”

mentioning

confidence: 99%

Dipslide cultures in the investigation of suprapubic urinary bladder aspirates of infants and children.

Kostiala¹,

Pylkkänen²

1980

J Clin Pathol

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SUMMARY Suprapubic aspirates (SPAs) of the urinary bladder obtained from 793 infants and children with suspected urinary tract infection were grown on dipslides inoculated at the bedside as well as being cultured aerobically and anaerobically. In general, the findings on dipslides were in good agreement with those in the other culture media. The majority of the 403 dipslide-positive samples contained 105 or more bacteria/ml; only in 2 % was bacterial density less than 0I/ml. There were five specimens with no growth on dipslides; however, an organism considered to be significant for the patient was isolated in the other cultures. Thus dipslides proved to be adequate for the culture of most SPAs, but samples from patients with urological problems should be studied by more complete methods.Significant bacteriuria is generally held to occur when there are at least 105 bacteria/ml ofclean-voided urine, and findings of 103/ml or less are considered to be insignificant. However, any amount of bacteria, excluding common skin contaminants, in a suprapubic aspirate (SPA) of the urinary bladder is thought to be significant.' In certain cases, only suprapubic aspiration of urine seems to offer a correct diagnosis of urinary tract infection, especially in infants and children, and complications are rare.2 4 Dipslide cultures have been found useful for the diagnosis and follow-up of urinary tract infections from clean-voided urine specimens.35-8 However, low bacterial counts have not always been reliably detected on dipslides, which of course, is insignificant for clean-voided urine.6 Therefore, we decided to investigate the occurrence of low bacterial counts in SPAs of infants and children, and to compare findings on dipslides and plates inoculated at the bedside with those of aerobic and anaerobic cultures from SPA samples taken to the laboratory in a transport medium. Material and methods PATIENTSIn the first part of the study, 469 infants and children, aged from 1 day to 15 years, were studied in the

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Dip-slide: an aid to quantitative urine culture in general practice.

Cited by 100 publications

References 7 publications

The Dip-Slide: a modified dip-inoculum transport medium for the laboratory diagnosis of infections of the urinary tract

The Dip-Slide: a modified dip-inoculum transport medium for the laboratory diagnosis of infections of the urinary tract

Rezidivierende Harnweginfektion der Frau — Diagnostik

Dipslide cultures in the investigation of suprapubic urinary bladder aspirates of infants and children.

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